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《BULLETIN OF HUNAN MEDICAL UNIVERSITY》 1995-05
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A MTT METHOD FOR DETERMINATION OF TOTAL SOD ACTIVITY IN HUMAN ERYTHROCYTES

Nie Shongxing;Zhao Shuangxing(Deparment of parasitology,Human Medical University  
he conventional nitroblue tetrazolium(NBT)method to determine the total activity ofsuperoxide dismutase(SOD) in human erythrocytes was modified by using thiazolyl blue(MTT)instead of NBT,since the higher solubility of MTT could markedly increase the re-producibility of the determination, Through photochemical reaction,MTT could be convert-ed into formazan blue which is defficult to form precipitate and thus the time for experimentcould be duely prolonged. After the standard SOD curve was delineated and the light absorp-tion of a sample at the waveleugth of 340 nm was measured,the total SOD activity in humanerythrocytes could be calculated according to the formula:SOD U/ml/g Hb=60C/36.8A.The total SOD activity of erythrocytes in 240 normal suhects was 81.9±15.7U/ml/g Hb.We believe that MTT method is simple and easy to perform,economic in its expenses andquite reproducible when it is used to determine the total SOD activity of human erythro-cytes.
【CateGory Index】: Q554.9
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【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Yu-ling~(1),QI Jun-sheng~(1),WANG Yuan-liang~(2),ZHANG Wen~(3) (1.Department of Chemical Engineering,Chongqing Three Gorges University, Wanzhou 404000,China;2.College of Bioengineering,Chongqing University,Chongqing 400030,China;3.Department of Society Development,Chongqing Municipal Science and Technology Commission,Chongqing 400175,China);Effect of SOD on Apoptotsis of Arsenic Compound Induced[J];Journal of Chongqing University(Natural Science Edition);2005-09
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 DENG You ping, LIN Chen, ZHANG Xue yan, et al.National Laboratory of Molecular Oncology,Cancer Institute,Chinese Academy of Medical Sciences,Peking Union Medical College, Beijing 100021, P.R. China;Studies on arsenic trioxide induced human pulmonary adenocarcinoma GLC-82 cell apoptosis and its molecular mechanisms[J];CHINESE JOURNAL OF CANCER;1999-05
2 WANG Zhan xiang,LIAO Jun xian, XU Yong gang, et al. Xiyuan Hospital, China Academy of Traditional Chinese Medicine,(Beijing,100091) $$$$[;INFLUENCE OF ANTIOXIDANTS ON APOPTOSIS INDUCED BY ARSENIC TRIOXIDE[J];JOURNAL OF LEUKEMIA;2000-03
3 Zhang Peng;Hu Longhu;Zhau Jin;et al Ist Clinical College of Haerbin Medical University(Haerbin 150000);STUDY ON THE ACTION MODE OF ARSENIC TRIOXIDE INJECTIONIN TREATMENT OF ACUTE PROMYELOCYTIC LEUKEMIA[J];JOURNAL OF LEUKEMIA;1996-03
4 WEN Qin-sheng, LUO Jun-qing, JIAO Cheng-song (Department of Gastroenterology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China);Arsenic trioxide induces apoptosis in liver cancer cell HepG_2[J];Acta Academiae Medicine Militaris Tertiae;2001-08
5 ZHANG Bao, MO Yong-yan, ZHOU Mei, CHEN Yuan (Research Laboratory of Free Radical Medicine, First Military Medical University, Guangzhou 510515, China);Overproduction of manganese superoxide dismutase modulates H_2O_2-induced apoptosis in transfected human PC12 cells[J];Journal of First Military Medical University;2000-06
6 CHEN Xin Bin 1 * , YANG Yan 1, ZHU Shen Jie 2, GUI Ming De 2 [WT5”BX](1. College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China; 2. Department of Chemistry, Nankai University, Tianjin 300071, China;Studies on the Substituent Effects of New Schiff Base Complexes on the Catalytic Monooxygenation of Cyclohexane[J];Chemical Research In Chinese Universities;2000-11
7 YANG Da Cheng 1, , ZHANG Guo Lin 2, YANG Yu 3, ZHONG Yu Guo 3 [WT5”BX](1. School of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing 400715, China; 2. School of Pharmacy, Zhejiang University, Hangzhou 310031;The Mannich Reaction of 4-Methylacetophenone with Aromatic Aldehydes and Aromatic Amines[J];Chemical Research In Chinese Universities;2000-11
8 YANG Guang-lie (Nanjing Jiangnan Optics and Electronics(Group)Co.,Ltd.,Nanjing 210037,China);Fluorescence and fluorescence microscope[J];Optical Instruments;2001-02
9 LIU Tie fu,LIANG Tao (Department of Digestive Disease, First Affiliated Hospital of Harbin Medical University;Harbin 150001,China);Apoptosis Induced by As_2O_3 to Liver Cancer Line Hep201[J];Heilongjiang Medical Journal;2001-02
10 ZHANG Peng; LI Ming-xun; LIU Peng; SUN Shu-juan; et al(Department of Hematology, The First Affiliated Hospital, Harbin Medical University,Harbin 150001, China);Effects of mercuric chloride (HgCl_2) on K_(562) and HEL cell lines[J];JOURNAL OF HARBIN MEDICAL UNIVERSITY;1999-04
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 ZHU Hong-xia et al(Nanjing University of Information Science and Technology,Nanjing,Jiangsu 210044);Effect of Combined Pollution on Seed Germination and Enzyme Activity of Rice[J];Journal of Anhui Agricultural Sciences;2008-26
2 YIN Bin-xia, WANG Xiang-ling, ZHAO Li-hua, et al (The Second Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China);Detecting Superoxide Dismutase Activity in the Red Blood Cell by Automatic Biochemical Analyzer[J];Journal of Practical Medical Techniques;2004-16
3 GAN Yao-kun,DUAN Li-ju,YANG Xu ( Associate Professor,Department of Chemistry and Biology,Yulin Normal College,Yulin,53700) (M.A.student,Lab.of Enviromental Sience,College of Life Sience, Central China Normal University,Wuhan,430079) (Professor,Lab.of Enviromental Sience,College of Life Sience, Central China Normal University,Wuhan,430079);Studies on the influence of external substances on activity of blood superoxide dismutase[J];Journal of Yulin Teachers College;2003-04
4 WANG Shu-hong~1, WANG Yi-lei~1, ZHANG Zhao-xia~2,WENG Chao-hong~1, ZOU Zhi-hua~1, ZHANG Zi-ping~3 (1.Fishery College, Aquaculture Biotechnology Institute, Jimei University, Xiamen 361021, China; 2. Oceanary Department, Xiamen University, Xiamen 361005, China; 3. Department of Fisheries and Wildlife, Michigen State University, USA);Activities of selected enzymes in cell free haemolymph from abalone (Haliotis diversicolor) injected with E. coli or Vibrio parahemolyticus[J];Journal of Fishery Sciences of China;2004-01
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