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《BULLETIN OF HUNAN MEDICAL UNIVERSITY》 1998-02
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Culture of murine HPP-CFC in vitro using conditioned mediumsubstitute for recombinant growth factors

Xia Tian Jiang Dezhao(Research Laboratory of Blood Physiology, Hunan Medical University, Changsha 410078)  
Objects: To study the clonal growth on single layer agar of high proliferative potential colonyforming cells(HPP-CFC) of bone marrow obtained from both normal mice(NBM) and mice treated 2 days earlier with 5fluorouracil(FU2 BM) using conditioned media. Materials and methods: Using liquid cell culture to harvert WEHI3CM and L929CM as the substitutes for IL3 and MCSF respectively; using semisolid colture to assay HPCFC in vitro. Results: ① the combination of WEHI3CM and L929CM produced HPPCFC in vitro, and the addtion of rhIL1α, rhIL6 and rmGMCSF further enhanced HPPCFC colony formation; ② HPPCFC was enriched in the FU2 BM. Morphological investigation showed that the HPPCFC colony was large and compact, great than 0.5mm in diameter and contained more than 50,000 cells, most of which were macrophage like cells. The hydroxyurea suicide rate for HPPCFC was less than 10%. Conclusion: The results suggested that: ① WEHI3CM and L929CM can be used as the substitutes of IL3 and MCSF to stimulate the formation of HPPCFC; ② FU can enrich HPPCFC of the mice.
【Fund】: 湖南省科委基金
【CateGory Index】: Q343.6,
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Xue Jun JIANG Li Zhen WANG Xin Ming, HAN Zhong Chao (Shanghai Institute of Cell Biology, Academia Sinica, Shanghai 200031);A STUDY OF MEGAKARYOCYTES DIFFERENTIATED FROM MURINE HIGH PROLIFERATIVE-POTENTIAL COLONY-FORMING CELLS (HPP-CFC) IN VITRO[J];Chinese Journal of Coll Biology;1995-04
2 WANG Cheng-Long LI Wei-Min WANG Qi-Ru (Experimental Hemophysiology Laboratory, Hunan Medical University Changsha 410078);BIOLOGICAL CHARACTERISTICS OF L929 CELL LINE[J];Journal of Experimental Hematology;1995-04
【Co-citations】
Chinese Journal Full-text Database 3 Hits
1 Huang Xiaogin Zhu BideDepartment of Anatomy, Sichuan Continuing EducationCollege of Medical Sciences, Chengdu 610041;An Experimental Study on L-cell HRF Induced by TSPG[J];Sichuan Journal of Auatomy;1998-04
2 Huang Xiaoqin,Zhu Bide∥Sichuan Continuing Education College of Medical Science,Cheng du 610041;The Experimental Study on L cell Regulate Hematopoiete Effected by IL 1[J];JOURNAL OF SICHUAN CONTINUING EDUCATION COLLEGE OF MEDICAL SCIENCES;1999-03
3 Xia Tian, Jiang Dezhao (Department of Blood Physiology, Hunan Medical University, Changsha,410078);EFFECTS OF LITHIUM ON THE PROLIFERATION OF MURINE HIGH PROLIFERATIVE POTENTIAL COLONY FORMING CELLS AND GRANULOCYTE MACROPHAGE COLONY FORMING UNIT IN VITRO[J];CHINESE JOURNAL OF APPLIED PHYSIOLOGY;1997-03
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