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《Journa of Henan Medical University》 2002-06
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Effect of antibiotics and phosphinothricinic on growth of Dunaliella Salina

ZHENG Heming 1,2) , XUE Lexun 1) 1)Cell Biology Laboratory, Medical College, Zhengzhou University, Zhengzhou 450052 2)Anti Epidemic Station of Henan Province, Zhengzhou 450003  
Aim: To investigate the sensitivity of Dunaliella Salina (D.salina) to the common select reagents. Methods: In the solid and liquid culture media, reagents were added to observe the effect of them on D.salina growth. Results: The study revealed that Kanamycin, Penicillin, Streptomycin or Spectinomycin at 1 600 mg/L could not completely inhibit the growth of D.Salina.However, Rifampin at 400 mg/L could inhibit the grawth of D.salina was most sensitive to Phosphinothricin (PPT), which killed D.salina when PPT was at a concentration of 5 mg/L. Conclusion: PPT should be used when phosphinotricin herbicide resistance (BAR) gene is a selectable marker in the genetic engineering of the D.salina.
【Fund】: 河南省重大科技攻关资助项目  0 12 2 0 3 2 5 0 0 ;; 河南省杰出人才创新基金资助项目  0 2 2 10 0 190 0
【CateGory Index】: Q943
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