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《Journal of Zhengzhou University(Science Medical)》 2003-05
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Detection of aberrant methylation of p16 gene in lung tumor tissues,corresponding bronchoalveolar lavage fluid, and sputum by methylation-specific PCR

YANG Liping 1) , ZHANG Shulin 2) , LIU Wanggen 3) , HE Yi 4) 1)Subject of Biochemistry and Molecular Biology, Henan Chinese Traditional Medical College,Zhengzhou 450003 2)Department of Molecular Biology,Chest Hospital of Henan Province,Zhengzhou 450003 3)Subject of Histology and Embryology, Henan Chinese Traditional Medical College,Zhengzhou 450003 4)Department of Thoracic Surgery, Henan Provincial People's Hospital, Zhengzhou 450003  
Aim:To explore the clinical value of methylation specific PCR (MSP) on the diagnosis of Lung cancer. Methods:Pathological tissues taken in operation, corresponding bronchoalveolar lavage fluid (BALF), and sputum were obtained from 56 hospitalized patients, among whom 32 were lung cancer, and 24 were benign lung diseases.After DNA extracting and PCR amplification, the PCR products were stained with EB and observed under ultraviolet lamp.Results:Among 32 lung cancer tissues,14(43.8%) were found abnormal methylation in promoter area of p16 gene,9(64.3%), in corresponding BALF, and 5(35.8%), in corresponding sputum. While among 24 cases of benign lung diseases no p16 gene methylation was found in pathological tissues taken in operation, corresponding BALF or sputum. Conclusion:MSP has specificity on the detection of abnormal methylation of P16 gene in BALF and sputum of patients with lung cancer. This suggests that MSP might be a promising technique for lung cancer early diagnosis
【Fund】: 河南省科技攻关资助项目 0 0 40 2 2 0 0 0
【CateGory Index】: R734.2
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