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《Journal of Zhengzhou University(Science Medical)》 2004-01
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Amplification of atpA gene fragment from chloroplast of Dunaliella salina by PCR

HOU Guiqin, WANG Ning, XIE Hua, JIANG Guozhong, CHAI Yurong, WANG Tianyun, XUE Lexun Laboratory for Cell Biology, Zhengzhou University, Zhengzhou 450052  
Aim: To clone atpA gene fragment from the chloroplast of Dunaliella salina. Methods: According to conserved motif of the homologous amino acid sequence of about ten kinds of algae, we designed a pair of degenerate primers and amplified atpA gene fragment from the chloroplast of Dunaliella salina by PCR technique.The resulting PCR products were inserted into pMD-18 T-vector,and then transformed into E.coli JM109. Positive colonies were selected to determine their sequences. Homologous analysis of the deduced amino acid sequence were performed by BLAST and subsequently compared with GenBank data. Results: The nucleotide sequences were 405 bp which encoded 135 amino acid. The sequence shared high homology with atpA, with identity 92%, 88%, 87%, 86% and 85% to Chlamydomonas reinhardtii, Chlorella vulgaris, Mesostigma viride, Nephroselmis olivacea and Cyanidioschyzon merolae, respectively. Conclusion: It can be concluded that the cloned sequence is atpA gene fragment from the chloroplast of Dunaliella salina.
【Fund】: 国家高技术研究发展 ( 863 )计划  2 0 0 2AA62 80 5 0 ;; 河南省重大科技攻关基金资助项目  0 12 2 0 3 2 5 0 0 ;; 河南省杰出人才创新基金资助项目  0 2 2 10 0 190 0
【CateGory Index】: Q943
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