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《Journal of Zhengzhou University(Science Medical)》 2004-03
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Cloning and identification of a candidate novel gene IDD01 associated with hepatocellular carcinoma

CHEN Xiangyu 1) , LI Jiansheng 2) , DUAN Fangling 1) , LI Yulong 1) ,MA Jun 1) ,XUE Lexun 3) , ZENG Yanli 1) , SUN Yan 1) ,SUN Yan 1) 1) Institute of Digestive Disease, Zhengzhou University, Zhengzhou 450003 2) Department of Gastroenterology, the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052 3)Laboratory for Cell Biology,Zhengzhou University, Zhengzhou 450052  
Aim:To observe the mRNA abundance of expression sequence tag(EST) which was obtained from hepatocellular carcinoma(HCC) with suppression subtractive hybridization (SSH), and cloning its full length of cDNA. Methods: Semi quantitative RT PCR was conducted to analyze the expression of EST in tumor and adjacent noncancerous tissues surgically removed from 21 patients with HCC. The 5' and 3' ends of EST were amplified with rapid amplification of cDNA ends(RACE), and sequencing was conducted to obtain its full length. GenBank was searched for its homology. Results: Statisticallly significant difference of mRNA abundance was observed between tumor and adjacent noncancerous tissues( P 0.05). A 1 333 bp sequence was obtained, which obeyed the Kozak rules, and contained an open reading frame(ORF). BLAST analysis showed that it maybe a novel gene. Conclusion: A candidate novel gene IDD01 associated with HCC is obtained, which provides data for further study of its function in HCC.
【Fund】: 河南省医学科技创新人才工程项目  2 0 0 10 90 12 ;; 郑州大学“十五”“2 11工程”重点学科建设项目 河南省教重办 ( 2 0 0 2 )第 2号
【CateGory Index】: R735.7
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2 ZHU Wu-ling, DUAN Fang-ling, LIU Dong-Hang, FAN Bing-lin, ZHANG Ling,GAO Tian-hui, CHEN Xiang-yu.Institute of Digestive Diseases,Zhengzhou University,Zhengzhou 450003,China;Cloning the genes differently expressed in human hepatocel-lular carcinoma by suppression subtractive hybridization[J];Chinese Journal of Hepatology;2003-03
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【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 WANG Yu;CHEN Wei;LI Xu;ZHANG Hong;ZHANG Xiao-qin;SHI Ying-li;Medical Experiment Center,Shaanxi University of Chinese Medicine;Center for Translational Medicine,First Affiliated Hospital,College of Medical Sciences,Xi'an JiaotongUniversity;;Construction of eukaryotic expression vector of UCA1a(CUDR) gene and its expression in bladder cancer UM-UC-2 cells[J];Journal of Jilin University(Medicine Edition);2014-03
2 WANG Yu;CHEN Wei;LI Xu;Research Center,Shaanxi University of Chinese Medicine;Center for Translational Medicine,First Affiliated Hospital,College of Medicine,Xi'an Jiaotong University;;UCA1,Its Variant Transcripts and Bladder Cancer[J];Chinese Journal of Biochemistry and Molecular Biology;2014-07
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Ma Qingjiu *,Wu Jinsheng,Gao Deming,et al.\ \ *General Surgery Department of Tang Du Hospital,Fourth Military Medical University,Xi'an,710038;Percutaneous transhepatic hepatocarcinoma ablation with radio frequency by using a clustered electrode technique.[J];CHINESE JOURNAL OF PRACTICAL SURGERY;1999-10
2 ZHANG Jie, WANG Keshen, CONG Shuying, et al. Department of Cell Biology, School of Basic Medical Sciences, Peking University, Beijing 100083, China [;Correlation of c-myc gene amplification, MTS1/p16 gene alternation, and HBV infection in human hepatocellular carcinoma[J];Chinese Journal of Hepatology;2001-05
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