Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Zhengzhou University(Science Medical)》 2004-03
Add to Favorite Get Latest Update

Cloning and identification of a candidate novel gene IDD01 associated with hepatocellular carcinoma

CHEN Xiangyu 1) , LI Jiansheng 2) , DUAN Fangling 1) , LI Yulong 1) ,MA Jun 1) ,XUE Lexun 3) , ZENG Yanli 1) , SUN Yan 1) ,SUN Yan 1) 1) Institute of Digestive Disease, Zhengzhou University, Zhengzhou 450003 2) Department of Gastroenterology, the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052 3)Laboratory for Cell Biology,Zhengzhou University, Zhengzhou 450052  
Aim:To observe the mRNA abundance of expression sequence tag(EST) which was obtained from hepatocellular carcinoma(HCC) with suppression subtractive hybridization (SSH), and cloning its full length of cDNA. Methods: Semi quantitative RT PCR was conducted to analyze the expression of EST in tumor and adjacent noncancerous tissues surgically removed from 21 patients with HCC. The 5' and 3' ends of EST were amplified with rapid amplification of cDNA ends(RACE), and sequencing was conducted to obtain its full length. GenBank was searched for its homology. Results: Statisticallly significant difference of mRNA abundance was observed between tumor and adjacent noncancerous tissues( P 0.05). A 1 333 bp sequence was obtained, which obeyed the Kozak rules, and contained an open reading frame(ORF). BLAST analysis showed that it maybe a novel gene. Conclusion: A candidate novel gene IDD01 associated with HCC is obtained, which provides data for further study of its function in HCC.
【Fund】: 河南省医学科技创新人才工程项目  2 0 0 10 90 12 ;; 郑州大学“十五”“2 11工程”重点学科建设项目 河南省教重办 ( 2 0 0 2 )第 2号
【CateGory Index】: R735.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 WANG Yu,CHEN Wei,LI Xu(Center for Molecular Medicine,the First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China);Cloning of the full-length cDNA sequence of a novel human UCA1 spliced variant[J];Journal of Xi'an Jiaotong University(Medical Sciences);2012-01
【Citations】
Chinese Journal Full-text Database 3 Hits
1 CUI Shu-zhong, LIU Hai-ying, TANG Yun-qiang, HU Wei-min, WANG Jia-kang, LI Jian-chang, SONG Tao, LIN Sheng-qu Guangzhou Tumor Hospital and Clinical Center for Cancer Research of Guangzhou Medical College, Guangzhou 510095, China;Radiofrequency thermal ablation combined with arterial embolization for primary liver cancer[J];Journal of First Military Medical University;2003-10
2 ZHU Wu-ling, DUAN Fang-ling, LIU Dong-Hang, FAN Bing-lin, ZHANG Ling,GAO Tian-hui, CHEN Xiang-yu.Institute of Digestive Diseases,Zhengzhou University,Zhengzhou 450003,China;Cloning the genes differently expressed in human hepatocel-lular carcinoma by suppression subtractive hybridization[J];Chinese Journal of Hepatology;2003-03
3 DONG Zhi_wei1,QIAO You_lin1,LI Lian_di2,et al.(1.Cancer Institute,CAMS,Beijing 100021,China;2.The National Cancer Research and Control Office,Beijing 100021,China);Report of Chinese Cancer Control Strategy[J];Bulletin of Chinese Cancer;2002-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Min Rui, Li Mei Mei, Yin Kai Sheng Department of Pulmonology,The First Affiliated Hospital of Nanjing Medical University,Nanjing, Jiangsu,210029,P.R.China;Autofluorescence Bronchoscopy for Early Localization of Lung Cancer[J];Chinese Journal of Cancer;2003-10
2 Fan Bing-Lin1, Zhu Wu-Ling1, Zou Guo-Lin2, Luo Guo-Sheng1, Xu Chun- Lei1, Zhao Wei-Xing1 1.Department of Basic Medicine, Xinxiang Medical College, Xinxiang,Henan,453003, P.R.China 2.Department of Biochemistry, College of Life Sciences, Wuhan University, Wuhan,Hubei,430072, P.R.China;Cloning and Identification of Fibrinogen Gamma Polypeptide (FGG) Gene Differentially Expressed in Human Hepatocellular Carcinoma[J];Chinese Journal of Cancer;2004-03
3 CHEN Chuang1,CHEN Li-Qin2,YANG Guo-Liang1,LI Yan1 1. Department of Oncology,Zhongnan Hospital/Cancer Center,Wuhan University,Wuhan,Hubei,430071,P. R. China 2. Department of Oncology,Central Hospital of Xianning City,Xianning,Hubei,437100,P. R. China;Value of Tumor Markers in Diagnosing and Monitoring Colorectal Cancer and Strategies for Further Improvement: Analysis of 130 Cases[J];Chinese Journal of Cancer;2007-11
4 LIN Miao-Xia1, WEN Zhuo-Fu1, FENG Zhi-Ying2, HE Dan21. Department of Gastroenterology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, 510630, P.R.China 2. Department of Pathology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, 510630, P.R.China;Expression and Significance of Bmi-1 and Ki67 in Colorectal Carcinoma Tissues[J];Chinese Journal of Cancer;2008-12
5 QIU Hui,ZHOU QI(Chongqing Cancer Institute,Chongqing 400030,China);Survey of incidence and mortality status of malignant tumors in Chongqing City[J];Chongqing Medicine;2008-22
6 ZHOU Yuan,WANG Dong,ZHANG Chuan-sheng,HAN Kai-bao,YE Yu-kun(Department of Cardiothoracic Surgery,the 81th Hospital of PLA,Nanjing 210002,Jiangsu,China);Clinical significance of preoperatively pathological diagnosis of lung cancer[J];Military Medical Journal of Southeast China;2008-02
7 CHEN Xiang yu 1, DUAN Fang ling 1, LI Jian sheng 2, MA Jun 1, XUE Le xun 3 ( 1Institute of Digestive Diseases, Zhengzhou University, Zhengzhou 450003; 2Department of Gastroenterology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052; 3Laboratory for Cell Biology, Zhengzhou University, Zhengzhou 450052, Henan Province, China);Construction and expression of prokaryotic vector of a novel candidate gene IDD01 associated with hepatocellular carcinoma[J];Acta Academiae Medicinae Militaris Tertiae;2004-10
8 Qiu Zonglin1,Xu Qiang2,Zhang Ting2,Wang Yongfeng2,Chen Xiangmei2,Ye Bin1,3,Lu Fengmin2(1Department of Pathogenic Biology,3Research Center for Molecular Medicine and Tumor,College of Basic Medical Sciences,Chongqing Medical University,Chongqing,400016;2Department of Microbiology,Peking University Health Science Center,Beijing,1001913,China);Methylation of HHIP gene promoter in esophageal cancer cell lines[J];Journal of Third Military Medical University;2011-05
9 ZHANG Hui-Zhen1, BA Yue1, YANG Ji-Yao2, FAN Qing-Tang1, WU Yi-Ming1 1College of Public Health, 2Basic Medical College, Zhengzhou University, Zhengzhou 450001, China;Screening and identification of lung cancer associated proteins[J];Journal of the Fourth Military Medical University;2007-01
10 Wanqing CHEN, Siwei ZHANG, Xiaonong ZOU National Office for Cancer Prevention and Control, Beijing 100021, China;Estimation and Projection of Lung Cancer Incidence and Mortality in China[J];Chinese Journal of Lung Cancer;2010-05
China Proceedings of conference Full-text Database 3 Hits
1 Xiao-di ZHANG,Wen CHEN Institute of Soft Matter Mechanics,Department of Engineering Mechanics,Hohai University,1 Xikang Road Nanjing,Jiangsu 210098,China Xing CAI Simula Research Laboratory,1325 Lysaker,Oslo,Norway;SIMULATING FREQUENCY-DEPENDENT DISSIPATION IN THE CARI TECHNIQUE FOR BREAST TUMORS USING THE MODIFIED SZABO'S WAVE MODEL[A];[C];2008
2 ;“知识沟假设”在我国城乡癌症传播中的实证研究[A];[C];2007
3 Jin Aishan;Liaoning Cancer Hospital;;A Dynamic Analysis on 89319 Cases of Malignant Tumors[A];[C];2013
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 YANG Yu-Cong,LI Xu,CHEN Wei Center of Laboratory Medicine,First Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710061,China;Screening and identification of heterogeneous phenotype-associated genes in human bladder cancer[J];Journal of Southern Medical University;2006-03
2 LIU Liping, LI Jie, WANG Cui, YAN Yunmeng, XUE Lexun Laboratory for Cell Biology,Department of Bioengineering,Zhengzhou University, Zhengzhou 450001;Construction of cDNA library of Dunaliella salina and cloning of kinesin like calmodulin-binding protein gene[J];Journal of Zhengzhou University(Medical Sciences);2010-06
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 WANG Yu;CHEN Wei;LI Xu;ZHANG Hong;ZHANG Xiao-qin;SHI Ying-li;Medical Experiment Center,Shaanxi University of Chinese Medicine;Center for Translational Medicine,First Affiliated Hospital,College of Medical Sciences,Xi'an JiaotongUniversity;;Construction of eukaryotic expression vector of UCA1a(CUDR) gene and its expression in bladder cancer UM-UC-2 cells[J];Journal of Jilin University(Medicine Edition);2014-03
2 WANG Yu;CHEN Wei;LI Xu;Research Center,Shaanxi University of Chinese Medicine;Center for Translational Medicine,First Affiliated Hospital,College of Medicine,Xi'an Jiaotong University;;UCA1,Its Variant Transcripts and Bladder Cancer[J];Chinese Journal of Biochemistry and Molecular Biology;2014-07
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Ma Qingjiu *,Wu Jinsheng,Gao Deming,et al.\ \ *General Surgery Department of Tang Du Hospital,Fourth Military Medical University,Xi'an,710038;Percutaneous transhepatic hepatocarcinoma ablation with radio frequency by using a clustered electrode technique.[J];CHINESE JOURNAL OF PRACTICAL SURGERY;1999-10
2 ZHANG Jie, WANG Keshen, CONG Shuying, et al. Department of Cell Biology, School of Basic Medical Sciences, Peking University, Beijing 100083, China [;Correlation of c-myc gene amplification, MTS1/p16 gene alternation, and HBV infection in human hepatocellular carcinoma[J];Chinese Journal of Hepatology;2001-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved