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《Journal of Zhengzhou University(Medical Sciences)》 2008-03
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Simultaneous determination of Aloin and Aloe-emodin in Aloe by HPLC

LU Chaoguo1),WANG Hongzhuan2),LI Yanhong1),LIU Guoji1),LUO Tingliang1) 1)Institute of Chemical Engineering,Zhengzhou University,Zhengzhou 450001 2)Nylon 66 Salt Company of China Shenma Group,Pingdingshan 467013  
Aim: To set up a method for simultaneous determination of Aloin and Aloe-emodin in Aloe by HPLC.Methods:Using a column of ZORBAXSA-C18(5 μm,200 mm×4.6 mm) with a mobile phase of methanol-water(60:40,volume ratio),Aloin was detected at 360 nm and Aloe-emodin,at 430 nm.Results: It was shown that Aloin had a good linear relationship within the range of 0.002~0.050 mmol/L(r=0.999 74) and a good precision degree(RSD=0.24%),and the average recovery was 102.5%(RSD=1.1%);Aloe-emodin had a good linear relationship within the range of 0.001~0.040 mmol/L(r=0.999 77) and a good precision degree(RSD=0.39%),and the average recovery was 101.4%(RSD=1.0%).Conclusion: The method is rapid,simple and accurate,and could be used for the simultaneous determination of Aloin and Aloe-emodin in Aloe.
【CateGory Index】: O657.72
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