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《Journal of Zhengzhou University(Medical Sciences)》 2009-02
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Effects of RNAi for target on PPARγ to suppress adipogenic differentiation of MSCs of rabbit induced by ethanol

WANG Shaohua1),WANG Yisheng1),LI Yuebai2),ZHAO Guoqiang3)1)Departmemt of Orthopaedic Surgery,the First Affiliated Hospital,Zhengzhou University;Open Laboratory of Unode Science of Clinical Medicine of Henan Province,Zhengzhou 450052 2)Department of Biochemistry,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 3)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001  
Aim:To observe effects of RNAi for the target on peroxisome proliferator-activated receptor-γ(PPARγ) suppress the adipogenic differentiation of bone marrow stromal cells (MSCs) induced by ethanol.Methods:2nd dynasty MSCs were selected and randomly separated into 7 groups.Group normal (N):the cells were treated without the adenovirous and alcohol as control. Group model (M):the cells were treated with 0.09 mol/L ethanol. Group empty infection (C0):the cells were treated with adenovirous 1×105 U and 0.09 mol/L ethanol. Group irrelative array to the siRNA (C1):the cells were treated with irrelative array to the siRNA adenovirous 1×105 U and 0.09 mol/L ethanol. Group RNA interference 1,2,3 (S1,S2,S3):the cells were correspondingly treated with relative siRNA adenovirous 1,2,3,1×105 U,and with 0.09 mol/L ethanol respectively. After the cells being treated 7 days,the expression of PPARγ mRNA was measured with RT-PCR for 7 groups. After 14 days,the content of triglyceride in cells was measured. After 21 days,the cells were stained with Sudan Ⅲ,and then the number of adipocytes was counted on a light microscope. Results:There was high expression of PPAR mRNA in the cells,the number of adipocytes in culture increased,and the content of triglyceride in cells increased in group M,C0,and C1,and there were significant differences compared with group N (P0.05). There was low expression of PPAR mRNA in the cells,the few adipocytes in culture,and the content of triglyceride in cells slightly increased in group S1,S2,and S3,and there were significant differences compared with group M,C0,and C1 (P0.05),as well as no significant differences compared with group N (P0.05).Conclusion:RNAi for the target on PPARγ could efficaciously suppress the adipogenic differentiation of the MSCs induced by alcohol,which establishes a fine experimental fundament for the prevention to the ethanol-induced osteonecrosis of the femoral head.
【Fund】: 国家自然科学基金资助项目30672128
【CateGory Index】: R681.8
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 WU Xuejian 1) , YIN Wanle 1) ,LI Yuebai 2) ,WANG Yisheng 1) 1)Department of Orthopaedics, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Department of Biochemistry, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052;Effects of alcohol on mRNA expression of PPARγ and osteocalcin in human bone marrow mesenhymal stem cells[J];Journal of Zhengzhou University(Medical Sciences);2006-06
2 WANG Yisheng1),ZHANG Chi1),LI Yuebai2),ZHAO Guoqiang3),WANG Shaohua1),LI Zhenwei1),YIN Li1),LIU Hongjian1) 1)Department of Orthopaedic Surgery,the First Affiliated Hospital,Zhengzhou University;the Open Key Clinical Medical Experimental Laboratory Institute of Universities of Henan Province,Zhengzhou 450052 2)Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 3)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001;Construction of pAdeno-siPPARγ[J];Journal of Zhengzhou University(Medical Sciences);2008-03
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 GUO Yue-xin1,MAO Cheng-gang1,LUO Qing2,ZHENG Gai-huan1,XU You-hua1(1Department of Hematology and Tumor,2Laboratory of Surgery,Institute of Pediatrics Children's Hospital,Chongqing Medical University,Chongqing 400014,China);Construction of recombinant adenovirus vector of Livin siRNA and its effects on proliferation,apoptosis and drug resistance of K562 cells[J];Acta Academiae Medicinae Militaris Tertiae;2009-14
2 WANG Yisheng1),ZHANG Chi1),LI Yuebai2),ZHAO Guoqiang3),WANG Shaohua1),LI Zhenwei1),YIN Li1),LIU Hongjian1) 1)Department of Orthopaedic Surgery,the First Affiliated Hospital,Zhengzhou University;the Open Key Clinical Medical Experimental Laboratory Institute of Universities of Henan Province,Zhengzhou 450052 2)Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 3)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001;Construction of pAdeno-siPPARγ[J];Journal of Zhengzhou University(Medical Sciences);2008-03
3 LIU Ming1),WANG Shaohua1),LI Yuebai2),ZHAO Guoqiang3),WANG Yisheng1)1)Departmemt of Orthopaedic Surgery,the First Affiliated Hospital,Zhengzhou University; Open Laboratory of Unode Science of Clinical Medicine of Henan Province,Zhengzhou 450052 2)Department of Biochemistry,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 3)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001;Effects of siRNA adenovirus for target on PPARγ to maintain osteogenic differentiation of MSCs of rabbit suppressed by ethanol[J];Journal of Zhengzhou University(Medical Sciences);2009-02
4 LI Zhenwei1),LI Yuebai2),WANG Yisheng1)1)Departmemt of Orthopaedic Surgery,the First Affiliated Hospital,Zhengzhou University;Open Laboratory of Unode Science of Clinical Medicine of Henan Province,Zhengzhou 450052 2)Department of Biochemistry,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001;Effects of RNAi for target on PPARγ to suppress adipogenic differentiation of MSCs of rabbit induced by steroid[J];Journal of Zhengzhou University(Medical Sciences);2009-02
5 WANG Yisheng1),WANG Xiaogang1),LI Yuebai2),WANG Shaohua1)1)Departmemt of Orthopaedic Surgery,the First Affiliated Hospital,Zhengzhou University; Open Laboratory of Unode Science of Clinical Medicine of Henan Province,Zhengzhou 450052 2)Department of Biochemistry,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001;Effects of RNAi for target on PPARγ to maintain osteogenic differentiation of MSCs of rabbit suppressed by steroid[J];Journal of Zhengzhou University(Medical Sciences);2009-02
6 WANG Yi-sheng,LI Yue-bai,Yin Li,LIU Pei-ling,LI Jun-wei,PI Guo-fu(Department of Orthopaedic Surgery,1st Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,PR China;Department of Biochemistry and Molecular Biology,Basic Medical College,Zhengzhou,450052,PR China;Zhengzhou Orthopaedic Hospital,Zhengzhou 450052.PR China);Experimental study to prevent alcohol-induced osteonecrosis of the femoral head with puerarin[J];Henan Medical Research;2007-04
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 LI Yuebai 1) , YIN Li 2) , WANG Yisheng 2) , CUI Quanjun 3) 1)Department of Biochemistry, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052 2)Department of Orthopaedic Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 3)Department of Orthopaedic Surgery, University of Virginia Health Sciences Center, Charlottesville USA 22908;An experimental study of ethanol-induced regulation of adipogenesis in NIH 3T3 fibroblasts[J];Journal of Zhengzhou University(Science Medical);2003-05
2 LI Yuebai 1) , WANG Yisheng 2) , Quanjun Cui 3) Gwo-Jaw Wang 3) Gary Balian 3) 1)Department of Biochemistry, Basic Medical College, Zhengzhou University, Zhengzhou 450052 2)Department of Orthopaedic Surgery,the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052 3)Department of Orthopaedic Surgery, School of Medicine, University of Virginia, Charlottesville,VA 22908 USA;Effect of alcohol on PPARγ and osteocalcin mRNA expression in multipotential stem cell from bone marrow[J];Journal of Zhengzhou University(Science Medical);2004-05
3 WU Xuejian 1) , YIN Wanle 1) ,LI Yuebai 2) ,WANG Yisheng 1) 1)Department of Orthopaedics, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Department of Biochemistry, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052;Effects of alcohol on mRNA expression of PPARγ and osteocalcin in human bone marrow mesenhymal stem cells[J];Journal of Zhengzhou University(Medical Sciences);2006-06
4 ;Progression of RNA interferenc application[J];Henan Medical Research;2007-03
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