Research about CFU-GM's Frozing Reserve of Human Umbilical Cord Blood
Zhang Ling Wei Zhi-wen Yin Zhen-yao (The Second Affiliated Hospital of Qingdao Medical College,Qingdao 266042)
Objective:To observe the injury of CFU-GM of human-umhilical cord blood when re- served in different temperature zone and the feasibility of effective reservation through long-period frozing. Methods:Dealing with umbilical blood-mading cells through the following traditional method as being made under room teroperature-programme frozing-reserving in liquid nitrogen for observing its activity and production rate of CFU-GM.Results:The result showed normal when the cells were reserved under room- temperature while decreased under-8℃ and-20℃.The production rate decreased respectively by 15.5% and 38.1% under the two temperature zone and the damage continued under-30% but stopped under-80℃.Conclusion:Umbilical bloed-mading ceils are damaged mainly between-8～-30℃ while no more damage under lower temperature in liquid nitrogen for long period,while proves the suitable of-80℃ reservation.
【CateGory Index】： R318.52