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《Henan Medical Research》 2010-03
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Application of CRS-PCR-RFLP to identify PON2 (rs12026) gene polymorphisms

WAGN Wei,YAO Wu,ZHOU Fang,LI Chun-yang,YAN Zhen,WU Yong-jun,WU Yi-ming(Department of Occupational Health and toxicology,School of Public Health,Zhengzhou University,Zhengzhou 450001,China)  
Objective: To develop a proper assay for identifying single nucleotide polymorphism(SNP) of PON2 gene.Methods: PCR primers were designed by create restriction site(CRS) method,then PCR-RFLP was adopted to identify the SNP in PON2 gene.Results: One primer pair can get target products containing PON2 SNP site by PCR,restriction enzymes BSMAI were adopted to identify the SNP site.The results of PCR and RFLP were good.Conclusion: It suggested that the method of detecting the SNP of PON2 by CRS-PCR-RFLP theory was facility,economy,and rapid.
【Fund】: 国家自然科学基金(30872095 30972457);; 河南省教育厅自然科学研究计划项目(2010A330005 2010B330003)
【CateGory Index】: R341
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