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《Journal of Hainan Medical University》 2014-04
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Influence of transcription factors RORγt on the balance of Th17/Treg in mice with acute lung injury

SUN Xue-feng;WANG-Jian;YANG Lin;WANG Zheng;Department of Thoracic Surgery,Shenzhen People's Hospital Affiliated to Jinan University;  
Objective:To study the influence of transcription factors RORγt on the balance of Th17/Treg in mice with acute lung injury.Methods:Totally 40male SPF kunming mice were selected and randomly divided into 4groups,intravenous saline group was given intravenous saline(n=10),three experiment groups were given endotoxin tail vein injection,experiment group 1was observed 2hours after the treatment,experiment group 2was observed 6hours after the treatment,experiment group 3was observed 12hours after the treatment.Total number of white blood cell count in bronchoalveolar lavage fluid(BALF)was analyzed,pathology examination of lung tissue were conducted;ratio of Th17and Treg cells in peripheral blood was calculated,contents of IL-17,IL-23,IFN- gamma,RORγt,expression of these factors in lung tissues were detected by RT-PCR,expression of RORγt was detected by real-time quantitative PCR method.Results:In peripheral blood samples,proportion of Th17cells were higher in experiment group 1,2and 3comparing with that in the control group(P0.01).Proportion of Treg cells were decreased;and Th17/Treg ratio increased in the three groups.Contents of RORγt in experiment group 2was significant higher that that in experiment group 1,experiment group 3,and control group, comparison between the latter two groups showed significant difference(P0.01).Conclusion:Expression of Th17and RORγt is involved with the occurrence and progression of acute lung injury.Decreased RORγt may inhibit differentiation and proliferation of Th17,determination of transcription factor RORγt may reflect the severity of acute lung injury.
【Fund】: 2013年度深圳市科技计划项目(201303004)~~
【CateGory Index】: R563.8
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