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《Journal of Elinical Research》 2005-05
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Improved Cultivation of HUVECs in Vitro and Their Identification

HU Ye-rong, DENG You-hua, JIANG Zhi-bin,et al(Department of Cardiothoracic Surgery, Second Xiang-ya Hospital ,Central South University Changsha410011, China)  
ObjectiveTo establish a better method for cultivating primary and subcultured HUVECs in vitro and identify the cultural cells, so as to provide seed cells for tissue engineering vascular grafts.Improved collagenase typeⅠ perfusion method was used to isolate HUVECs , then cultivated in RPMI-1640 containing fetal bovine serum and endothelial cell growth factors, identified the biology of HUVEC with the light microscope and immunohistochemical method.Isolated HUVECs grew to monolayer after 7~10 days by cultivation in vitro. They presented polygonal and arranged like pavement stones under the light microscope. Correlation antigens of human Ⅷ factor were positive.[Conclusion]Cells isolated by improved collagenase typeⅠ perfusion method was more and easy to survive .They was proved to be HUVECs and present relative biological functions.
【Fund】: 湖南省科技计划重大专项基金(04SK1005)
【CateGory Index】: R329.2
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