Determination of Ginsenoside Rb1 in Shenziling Capsule by HPLC
Wang Fenghua,Zhou Ying,Deng Xiaobin,Tian Shen,Zhang Ting(Affiliated Hospital of Prevention and Treatment Institute of Occupational Disease of Act of Labour Health in Hunan,Changsha 410007)
Objective: Develop a method to determine content of ginsenoside Rb1 Shenziling Capsule by HPLC.Methods It was tested by HPLC with Inertsil ODS-3(250 mm×4.6 mm,5 μm)column,mobile phase acetonitrile/purified water 34∶66,detection wavelength at 203 nm,the column temperature at 40 ℃ and current speed at 1.0 mL/min.Results Ginsenoside Rb1 showed good linear relationship at the range of 62.5-1000 μg/mL.(r2=0.9993),the average recovery was 99.95%,RSD%= 1.0%.Conclusion The HPLC method is simple,quick and accurate,which can be used for content determination of ginsenoside Rb1 in Shenziling Capsule.
【CateGory Index】： R286