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《Journal of Chinese Practical Diagnosis and Therapy》 2012-06
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Quantitative analysis of fetal sex-determining region of Y-chromosome gene by cell-free fetal nucleic acids in maternal plasma

HOU Qiao-fang,ZHANG Wei-hua,WU Dong,LIAO Shi-xiu (Institute of Medical Genetics,Henan Provincial People's Hospital,Zhengzhou 450003,China)  
Objective To explore the clinical application value of cell-free fetal nucleic acids to non-invasive prenatal diagnosis.Methods Real-time quantitative PCR and Taqman probes were used for amplification and quantitative analysis of sex-determining region of Y-chromosome(SRY) gene in maternal serum in 87 pregnancy women,including 61 mid-trimester(16~24 weeks) and 26 first trimester(11~14 weeks) pregnancy women.Results All the samples were amplified specifically and sensitively after pregnancy for over 13 weeks,with the specificity and sensitivity of 100% respectively.In the mid-trimester pregnancy group the average concentration of male fetal SRY gene was 155.46 copies/mL plasma and the measured concentration range was from 43.6 to 326.8 copies/mL plasma.In the first-trimester pregnancy group the average concentration of SRY gene was 26.42 copies/mL plasma,and the measured concentration range was from 14.3 to 73.3 copies/mL plasma.Conclusion Real-time PCR is a steady method to diagnose fetal sex in the first-trimester and mid-trimester pregnancy women by fetal cell free DNA.The program can be used as an effectively supplementary method in the prenatal diagnosis of sex-linked genetic disease.
【Fund】: 国家自然科学基金(81170581)
【CateGory Index】: R714.5
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【References】
Chinese Journal Full-text Database 3 Hits
1 QIU Jie,YANG Xiao-hua,FANG Hong,et al(Shenzhen Baoan Maternal and Child Health Hospital,Shenzhen,Guangdong 518133,P.R.China);Clinical value of screening fetal aneuploidy using a combination of maternal serum examination and massively parallel sequencing based non-invasive prenatal diagnosis[J];China Medical Engineering;2013-04
2 QIU Jie1,YANG Xiao-hua1,DENG Xi-di2,FANG Hong1,CHEN Xi1,WU Qiong-ling1.(1.Prenatal Diagnostic Center of Maternal and Children Care Service Hospital in Baoan,Shenzhen,518133;2.Liantang Branch of Chinese Medical Hospital in Luohu,Shenzh 518001);Clinical usage and comprehensive benefits analysis of noninvasive prenatal diagnosis combined with serum screening[J];Chinese Journal of Birth Health & Heredity;2013-07
3 Qiu Jie,Yang Xiao-hua,Fang Hong,et al;The Clinical Value of Maternal Serum Screening for Fetal Aneuploidy Combined Non-invasive Prenatal Test[J];Chinese Journal of Prenatal Diagnosis(Electronic Version);2013-02
【Citations】
Chinese Journal Full-text Database 3 Hits
1 WANG Wen-bo1,2,ZHANG Yi2,SUN Shu-han2 1.No.10 Student Team,Second Military Medical University,Shanghai 200433,China 2.Department of Medical Genetics,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433;Prenatal diagnosis value of free fetal DNA in maternal blood[J];Academic Journal of Second Military Medical University;2009-04
2 GAO Xuexiang,WEI Banghai,XU Haihong. Department of Laboratory,the Third People's Hospital of Hefei,Hefei 230022,China;Comparison among real-time PCR,PCR and bacterium culture in detection of Vibrio parahaemolyticus[J];Journal of Chinese Practical Diagnosis and Therapy;2009-09
3 WANG Li,WANG Tao,KANG Bing,et al. Medical Genetic Institute of Henan Provincial People's Hospital,Zhengzhou 450003,China;Prenatal gene diagnosis of 4 Duchenne muscular dystrophy pedigrees by PCR[J];Journal of Chinese Practical Diagnosis and Therapy;2010-02
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 DING Ning1,2,YAN Zhi-min1,HUA Hong1 (1.Department of Oral Medicine,Peking University School and Hospital of Stomatology,Beijing 100081,China;2.The Third Dental Center,Peking University School and Hospital of Stomatology,Beijing 100191,China);Detection of oral fungal flora in patients with primary Sjgren's syndrome by real-time PCR[J];Journal of Peking University(Health Sciences);2013-01
2 WANG Fengyu1),SUN Weiwei2),LI Congmin1),CHANG Mingxiu1),FENG Huigen2),MA Linxian2) 1)Henan Provincial Research Institute for Population and Family Planning,Henan Key Laboratory of Interference Technology for Newborn Genetic Diseases,Zhengzhou 450002 2)School of Biological Science and Technology,Xinxiang Medical University,Xinxiang 453000;Identification of deletions and duplications of the dystrophin gene in Duchenne muscular dystrophy patients from Henan by MLPA[J];Journal of Zhengzhou University(Medical Sciences);2011-06
3 LI Lin-hai1),SHI Yu-ling1),CHEN Li-dan1),ZENG Lan-lan1),WANG Lu-xia1),SUN Zhao-hui1),HE Jie-jing1),CHEN Jian-yun1),YANG Yong-quan1),ZENG Hua2),YUE Feng3)1) Department of Laboratory Medicine,Guangzhou General Hospital of Guangzhou Military Command,Guangzhou 510010,China;2) Department of Laboratory Medicine,the Second Affiliated Hospital to Sun Yat-sen University,Guangzhou 510120,China;3) Department of Laboratory Medicine,Guangdong Provincial Corps Hospital of Chinese People's Armed Forces,Guangzhou 510507,China;Real-time quantitative PCR for rapid detection of Clostridium perfringens[J];Journal of Chinese Practical Diagnosis and Therapy;2011-07
4 XU Sheng-yuan*,PAN Xiao-dong,SUN Li-yuan,LIN Jie,LIU Jun-tao,YAO Feng-xia, DU Lan-ping,WANG Lu-ya(*The Key Laboratory of Remodeling-Related Cardiovascular Diseases,Ministry of Education; Beijing Institute of Heart,Lung and Blood Vessel Diseases,Beijing Anzhen Hospital Affiliated to Capital Medical University,Beijing 100029,China);Low density lipoprotein receptor gene mutation of amniotic cell in the prenatal diagnosis of familial hypercholesterolemia[J];Journal of Chinese Practical Diagnosis and Therapy;2012-04
5 SHAN Zhu-Zhou,RAO Xiao-Peng,WANG Fang,LIU Jing(Qiandongnan Center for Disease Control and Prevention,Kaili 556000,China);Monitoring result of Hand-foot-mouth disease pathogens in Qiandongnan Prefecture,Guizhou in 2010[J];Chinese Journal of Health Laboratory Technology;2011-06
6 GAN Zhi-feng,FANG Xiao-wu,XIAO Ying,LIANG Shao-xia,ZHAO Jing,LI Hong-yan.(Boai Hospital Zhongshan,Guangdong 528403 China);Cell free fetal DNA from maternal plasma for β-thalassemia prenatal diagnosis[J];Chinese Journal of Birth Health & Heredity;2013-04
7 ZENG Mei-ling,FENG Li-wen,XU Dan-fen,HUANG Li-hua(Shunde Maternal and Child Health Hospital of Foshan,Shunde 528300,China);Clinical Analysis of Chromosomal Abnormalities of 413 Cases by Puncturing Umbilical Cords[J];Guide of China Medicine;2013-13
【Co-references】
Chinese Journal Full-text Database 9 Hits
1 YU Qiong,DENG Zhihui.Shenzhen Blood Center,Shenzhen,Guangdong 518035,P.R.China;Progress of research on non-invasive prenatal diagnosis by detecting the cell-free fetal DNA in maternal plasma[J];Experimental and Laboratory Medicine;2012-01
2 DENG Yan-hua, CHEN Hua-yun, DING Wei, ZHENG Yun, LI Ming (Daan Gene Diagnostic Center, Sun Yat-sen University, Guangzhou 510665, China);Non-Invasive Prenatal Diagnosis of Down's Syndrome[J];Journal of Tropical Medicine;2010-09
3 Li Yuzhi,Ren Jinghui,Lin Linhua et al Department of Prenatal Diagnosis,Shenzhen People's Hospital,Jinan University Second Clinical Medical College,Shenzhen 518020,China;Non-invasive Prenatal Diagnosis of Chromosome Aneuploidy by Massively Parallel Genomic Sequencing Technology[J];Acta Medicinae Universitatis Scientiae et Technologiae Huazhong;2012-04
4 JI Ying,ZHENG Xiao-ying.(Department of Social Medicine and Health Education,School of Public Health,Peking University Health Science Center,Beijing 100191,China);THE BURDEN OF BIRTH DEFECTS IN POOR AREAS[J];Modern Preventive Medicine;2011-02
5 BIAN Xu-ming,QI Qing-wei;Long way to go:prenatal screening and diagnosis of chromosomal abnormalities[J];Chinese Journal of Practical Gynecology and Obstetrics;2010-12
6 ;胎儿遗传特征与产前诊断[J];Chinese Journal of Practical Gynecology and Obstetrics;2010-12
7 GUO Qi-wei,ZHOU Yu-lin.(Molecular Diagnostic Lab,Center for Prenatal Diagnosis,Maternal and Children's Health Hospital of Xiamen,Xiamen,361003 P.R.China);Noninvasive prenatal diagnosis of Down syndrome[J];Chinese Journal of Birth Health & Heredity;2010-04
8 WU Qing-ming1,ZHOU Jin2.(1.Medical College of Shihezi University,Shihezi,Xinjiang 832000 China;2.Department of Obstetrics & Gynecology,Urumuqi General Hospital of Lanzhou Military Region,Urumuqi 830000,China);Advances in prenatal screening and prenatal diagnosis for birth defect[J];Chinese Journal of Birth Health & Heredity;2011-01
9 SU Chen1, ZHANG Yi2, SUN Shuhan2 (1.Biotechnology Company, Second Military Medical University, Shanghai 200433, China;2. Department of Medical Genetics, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433);The research progression of non-invasive prenatal diagnosis of Down’s syndrome based on the fetal genetic material[J];Journal of Molecular Diagnostics and Therapy;2011-02
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 Dong Min Li Jihong Wang Feng Department of Medical Genetics,Institute of Basic Sciences,CAMS & PUMC,Beijing 100005,China;The Strategy on Prenatal Diagnosis in Family with Sporadic DMD Case[J];Bulletin of Medical Research;2005-04
2 HUANG Wen 1, ZHANG Cheng 1, XIE You-mei 2, CHEN Song-lin 1, ZHANG Wei-xi 1, LU Xi-lin 1, YAO Xiao-li 1, ZENG Ying 1. 1(Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510080 P.R. China. 2(Department of Neurology, University of North Carolina, Chapel Hill, North Carolina, NC27599 USA);Carrier detection of Duchenne/Becker muscular dystrophy in Chinese families by microsatellite analysis[J];Chinese Journal of Medical Genetics;2004-03
3 Shang Shiqiang, Hong Wenlan, Yu Huimin, et al. Children′s Hospital,Zhejiang Medical University , Hangzhou 310003;Amplification of bacterial DNA by polymerase chain reaction(PCR)and typing by reverse hybridization[J];Chinese Journal of Laboratory Medicine;1998-05
4 Liu Xiumei (National Institute for Nutrition and Food Safety, China CDC, Beijing 100050);Studies on the techniques for the monitoring and controlling foodborne illness[J];Chinese Journal of Food Hygiene;2004-01
5 YANG Yu, ZHANG Cheng,QIU Wei, ZHONG Yue-gui et al. (Department of Neurology,the Third Affiliated Hospital,Sun Yat-Sen University, Guangzhou 510630);Multiplex polymerase chain reaction analysis by capillary electrophoresis for detecting DMD/BMD.[J];Chinese Journal of Birth Health & Heredity;2005-02
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