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《China Journal of Chinese Medicine》 2010-02
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Compare of Chlorogenic Acid in Gardeniae from Different Areas and in Various Prepared Products

Duan Qi1,Chen Huashi2 Guangdong Food and Drug Vocational College,Guangzhou,Guangdong,China 510520 Guangdong Kangmei Pharmaceutical Co.,Ltd,Puning,Guangdong,China 515300  
Objective:To develop a method using HPLC for determination content of Chlorogenic acid in Gardenia from different areas and in various prepared products.Methods:Zorbax XDB-C18(4.6 150 mm,5 μm),mobile phase:acetonitrile-1.1% acetic acid glacial((0-12 min:7:93;12-20 min:10:90)),detected wavelength:326 nm.The column temperature was maintained at 25℃.Results:In Gardenia from different areas and in various prepared products,chlorogenic acid content was gradually to decrease.Conclusion:The HPLC method is simply and suitable to control quantitative of Gardenia.Many facts show a influence on the contents of Chlorogenic acid in Gardenia from different areas and in various prepared products,it's good for quality control in Gardenia from prepared products.
【CateGory Index】: R284
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