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《Journal of Traditional Chinese Medicine University of Hunan》 2008-01
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Study of Lycoris aurea on induction and culture of adventitious root in vitro

PENG Fei,ZHONG Xiang-yun,TAN Zhao-yang,LEI Si-qi,LI Yun-yao(TCM University of Hunan,Changsha,Hunan 410208,China)  
Objective To investigate the induction and culturing condition of adventitious root of Lycoris aurea in vitro,and detect its active principle galanthamine.Methods The twin-squamas of Lycoris aurea was took as explant to be cultured in different mediums.The active galanthamine was detected in the adventitious roots.Results The callus could induce directly from twin-squamas in MS+2,4-D 2.0 mg/L;the callus in medium of IBA 1 mg/L+BA 0.1 mg/L was the best condition to induce rooting;and the activity-galanthamine detected with TLC was contained in the roots.Conclusions The adventitious root will be a new resource to obtain the galanthamine from culturing Lycoris aurea in vitro.
【Fund】: 湖南省卫生厅中医药科研基金项目(2006067)
【CateGory Index】: R282.2
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