Study on effect target of Maxing Shigan decoction on anti-influenza virus A in vitro

LU Fang-guo,HE Ying-chun,XIAO Zi-zeng,WU Can-rong,ZHANG Wei,LI Shan,ZHOU Xing,ZHOU Yi-xiang(TCM University of Hunan,Changsha,Hunan 410007,China)  

Objective To study the effect target of Maxing Shigan decoction(MXSGD) on anti-influenza Virus A in vitro.Methods The cell culture technique,the Hemagglutination reaction and MTT test were used to determine the effects of MXSGD on hemagglutination titer of influenza virus A and MDCK cells activities infected by influenza virus A with 4 different treatment methods.Results When the concentration of MXSGD for killing virus was from 3.19 mg/mL to 31.25 mg/mL,the hemagglutination titer was lower obviously compared with virus control group;from 15.63 mg/mL to 31.25 mg/mL,lower obviously compared with table Capsule Ribavirin group.When the concentration of MXSGD for interfering virus absorption was from 1.95 mg/mL to 31.25 mg/mL,the hemagglutination titer of influenza Virus A was lower obviously compared with the virus control group,from 7.81mg/mL to 31.25 mg/mL,lower obviously compared with the table Capsule Ribavirin group.When the concentration of MXSGD for protecting host cells was from 3.19mg/mL to 31.25mg/mL was lower obviously compared with virus control group,from 7.81mg/mL to 31.25mg/mL,lower obviously compared with the Capsule Ribavirin group.In the above three ways of administrating medicine,the treatment index(TI) of MXSGD on MDCK cell infected by influenza Virus A was higher than that in Capsule Ribavirin group;in the actions of MXSGD to inhibit influenza Virus proliferation,when the concentration of MXSGD was from 1.95 mg/mL to 31.25 mg/mL,the hemagglutination titer was lower obviously compared with Virus control group.Conclusion MXSGD is strongly inhibitory effects on influenza Virus A by directly killing virus A,interfering Influenza Virus A absorption,inhibiting virus proliferation and protecting the cells infected by influenza Virus A,and all the actions of the decoction is stronger than that of Capsule Ribavirin,except for the effect of inhibiting virus proliferation is not so effective to Capsule Ribavirin.

【Key Words】： Maxing Shigan decoction influenza Virus activity of cells hemagglutination titer effect target Herba Ephedrae Semen Armeniacae Amarum
【Fund】： 湖南省自然科学基金资助项目(06JJ2055);; 湖南省卫生厅科研课题(2006-56);; 湖南省科技计划项目(2007f4144)
【CateGory Index】： R285

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