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《Journal of Traditional Chinese Medicine University of Hunan》 2008-02
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DNA extraction from and optimization of RAPD reaction system for medical lily

TONG Qiao-zhen1,2,SHENG Xiao-bang1,LIU Xiang-dan2,ZHOU Ri-bao2*,WU Jia2(Hunan Agricultural University,Changsha,Hunan 410128,China)  
Objective To confine the best analysis method by RAPD of the medical lily.Methods DNA of medical lily was extracted from the fresh scale leaves and discussed the RAPD conditions.Results The system and program of RAPD for lily were as follows: The reactions were performed in a volume of 40 μL containing 40 ng of DNA,0.3 μmol/L primer,0.2 mmol/L dNTPs,1.7 mmol/L Mg2+,3 μL 10×PCR Buffer,1.5 U Taq enzyme;Thermal cycling was programmed by 1 cycle for 2 min at 94 ℃,followed by 40 cycles for 1min at 94 ℃,45 sec at 36 ℃,and 90 sec at 72 ℃ and finally,by 1 cycle for 10 min at 72 ℃,storied at 4 ℃.Conclusion The optimized reaction system and program of RAPD for medical lily has established.
【Fund】: 国家科技支撑项目(2006BAI06A11-06);; 湖南省教育厅资助项目(06C626)
【CateGory Index】: R284.2
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