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《Journal of Traditional Chinese Medicine University of Hunan》 2012-07
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Screening of the gene related to galantamine biosynthesis of Lycoris aurea Herb. by mRNA differential display

TANG Jin-feng,LU Yao-bang,GUI Lui-zi(TCM Uniwersity of Hunan,Changsha,Hunan 410208,China)  
Objective To isolate and clone the gene related to galantamine biosynthesis of Lycoris aurea Herb.Methods The specially expression cDNA fragments of the gene related to galantamine biosynthesis were screened by differential display polymerase chain reaction(DD-PCR) after extracting the total RNA from the pistils tissues and the scape tissues of the Lycoris aurea Herb cropped in July which had differential contents of the galantamine.Then these fragments were separately cloned to plasmid PMD19-T and subsequently underwent sequence analysis and homogenous comparison.Results 44 differential display bands were found in gene expression between the pistils tissues and the scape tissues of the Lycoris aurea Herb.According to the sequence analysis and homologenous comparison,two of them were confirmed to related with galantamine biosynthesis,one of them was possible related with galantamine transport regulation.Conclusion The three homologous sequences of Lycoris aurea screened by DD-PCR provide strong basis of galantamine synthetic pathways.
【Fund】: 湖南省自然科学基金资助项目(10JJ2024);; 湖南省教育厅科研基金资助项目(07A052)
【CateGory Index】: S567.239
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