Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Traditional Chinese Medicine University of Hunan》 2012-07
Add to Favorite Get Latest Update

Screening of the gene related to galantamine biosynthesis of Lycoris aurea Herb. by mRNA differential display

TANG Jin-feng,LU Yao-bang,GUI Lui-zi(TCM Uniwersity of Hunan,Changsha,Hunan 410208,China)  
Objective To isolate and clone the gene related to galantamine biosynthesis of Lycoris aurea Herb.Methods The specially expression cDNA fragments of the gene related to galantamine biosynthesis were screened by differential display polymerase chain reaction(DD-PCR) after extracting the total RNA from the pistils tissues and the scape tissues of the Lycoris aurea Herb cropped in July which had differential contents of the galantamine.Then these fragments were separately cloned to plasmid PMD19-T and subsequently underwent sequence analysis and homogenous comparison.Results 44 differential display bands were found in gene expression between the pistils tissues and the scape tissues of the Lycoris aurea Herb.According to the sequence analysis and homologenous comparison,two of them were confirmed to related with galantamine biosynthesis,one of them was possible related with galantamine transport regulation.Conclusion The three homologous sequences of Lycoris aurea screened by DD-PCR provide strong basis of galantamine synthetic pathways.
【Fund】: 湖南省自然科学基金资助项目(10JJ2024);; 湖南省教育厅科研基金资助项目(07A052)
【CateGory Index】: S567.239
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 GUI Liu-zi1,CUI Pei-wu1,PAN Qing-ping2,TANG Jin-feng3,LU Yao-bang4*(1.TCM University of Hunan,Changsha,Hunan 410208,China; 2.Key Laboratory of Modernization of Traditional Chinese Medicine of Hunan Province; 3.Guangdong Medical College,Dongguan,Guangdong 523808,China; 4.Laboratory of Nature and Effectiveness of Chinese Medicine,TCM University of Hunan,Changsha,Hunan 410208,China);Cloning and sequence analysis of LaCOMT in the biosynthesis pathway of galanthamine in Lycoris aurea[J];Journal of Traditional Chinese Medicine University of Hunan;2013-03
【Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Yong-cheng,GONG Zhen-hui,LI Da-wei(College of Horticulture,Northwest Agricultural and Forestry University,Yangling Shanxi 712100,China);Cloning of the Genes Resistant to Phytophthora Capsici in Pepper(Capsicum annuum L) by mRNA Differential Display Technique[J];Northern Horticulture;2008-12
2 XIAO Yan,PENG Fei,WANG Qing,LIU Jun(Faculty of Pharmacetics,TCM Univ.of Hunan,Changsha,410004,China);Study on Tissue Culture of Lycoris Aurea Herb[J];;2006-01
3 ZHAO Zhi-min,ZHONG Xiang-yun,YANG-Shuai,LU Yao-bang,PENG Fei(Hunan University of TCM,Changsha,Hunan 410208,China);Study on callus culture of Lycoris aurea[J];Journal of Traditional Chinese Medicine University of Hunan;2009-05
4 NIE Yuan-yuan,ZHOU Hai-xiang,PENG Fei,LU Yao-bang(Pharmaceutical College,TCM University of Hunan,Changsha,Hunan 410208,China);Determination of galanthamine from different parts of Lycoris aurea herb with HPLC[J];Journal of Traditional Chinese Medicine University of Hunan;2009-05
5 TANG Jin-feng,LU Yao-bang*,GUI Liu-zi(Pharmaceutical College,TCM University of Hunan,Changsha,Hunan 410208,China);Comparison of total RNA extraction between Lycoris aurea Herb Flowers and scapes[J];Journal of Traditional Chinese Medicine University of Hunan;2012-01
6 YUAN Ju-hong1,HU Mian-hao2,XIA Bing3 (1.Department of Landscape Architecture,Jiangxi University of Finance and Economics,Nanchang 330032,China;2.Resource and Environment Department of Jiangxi University of Finance and Economics,Nanchang 330032,China;3.Institute of Botany,Jiangsu Province & Chinese Academy of Sciences,Nanjing 210014,China);A Study on the Difference in Alkaloids Contents in Different Species of Lycoris[J];Acta Agriculturae Universitatis Jiangxiensis;2010-03
7 Tao Jie~1,Shan Ying~1,Jing Yingjun~1,Lu Xianyu~1,Li Desen~1,Xie Jianjun~2,Zhang Jie~2 (1.College of Life Sciences,Nankai University,Tianjin 300071,China; 2.Command Post of Controlling Locust In Tianjin,Tianjin 300380,China);Screening and Cloning of the Oriental Migratory Locust-related Resistance Gene by mRNA Differential Display[J];Acta Scientiarum Naturalium Universitatis Nankaiensis;2009-01
8 KONG Yang1,PENG Li2,CHEN Sheng1,TIAN Qin1,BAO Jin-Ku1(1.College of Life Sciences,Sichuan University,Chengdu 610064,China;2.Clinic of Joint Logistics Department,The Chengdu Military Area,Chengdu);The effect of temperatures,pH and denaturants on the conformation and biological activity of Lycoris aurea agglutinin[J];Journal of Sichuan University(Natural Science Edition);2008-06
9 WANG Xiao Yan WANG Peng Kai HUANG Min Ren ZHOU Jian HAN Zheng Min (College of Forest Resource and Environinent,Nanjing Forestry University,Nanjing 210037);THE LOCALIZATION OF GALANTHAMINE IN VEGETATIVE ORGAN OF LYCORIS AUREA HERB.[J];Journal of Molecular Cell Biology;2007-05
10 LIANG Hong-Wei1,2,WANG Chang-Zhong1,2,3,LI Zhong1,2,LUO Xiang-Zhong1,2,ZOU Gui-Wei1,2,3 1.Key Laboratory of Fish Germplasm Resources and Biotechnology Certificated by Ministry of Agriculture,Yangtze River Fisheries Re-search Institute,Chinese Academy of Fishery Sciences,Jingzhou 434000,China;2.Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;3.College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;Improvement of the silver-stained technique of polyacrylamide gel electrophoresis[J];Hereditas;2008-10
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SHAN Bao-en,LI Jun-xin,ZHANG Jing,LIANG Wen-jie,LI Hong-fen(Science and Research Center,The Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang050011,China);The Anti-tumor in Vitro Effect of Cortex Periplocae and Its Analogues Extracted[J];Carcinogenesis,Teratogenesis and Mutagenesis;2005-05
2 DUAN Nai-bin1,WANG Jian-cheng1,YU Tian-xiang2,KONG Su-ping3, LI Qun1,ZHANG Wen-lan1,DAI Shuang1(1.Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China; 2.Entry & Exit Inspection and Quarantine Bureau of Jiaxing,Jiaxing 314001,China; 3.Vegetable Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China);Development of EST-SSR Markers for Cultivar Identification of Festuca arundinacea[J];Shandong Agricultural Sciences;2010-10
3 LU Wen-yun et al (College of Chemistry and Environment, Guizhou University for Nationalities, Guiyang, Guizhou 550025);Compared Analysis of Total Alkaloids and Polysaccharides in Tubing Shoot and Wild Plant of Dendrobium Loddigesii[J];Journal of Anhui Agricultural Sciences;2006-08
4 TIAN Shi-lin et al (Huanghuai University,Zhumadian,Henan 463000);Determination and Comparison of Chlorogenic Acid in Leaf and Bark of Cortex[J];Journal of Anhui Agricultural Sciences;2006-13
5 YIN Shuai-wen et al(College of Life Science,Jinggangshan University,Ji'an,Jiangxi 343009);Study on Traditional Poisonous Pteridophyte in Jinggangshan Region[J];Journal of Anhui Agricultural Sciences;2007-08
6 WU Gong-qing et al (Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006);Study on the Extraction of Total RNA from Pinellia ternata (Thunb.) Berit Leaves[J];Journal of Anhui Agricultural Sciences;2008-08
7 ZHANG Xu et al(College of Agronomy,Sichuan Agricultural Universty,Yaan,Sichuan 625014);The Determination of Vitamins in the Leaf of Perilla L.by HPLC[J];Journal of Anhui Agricultural Sciences;2008-19
8 LI Jin-bu et al (Life Sciences College of Huaibei Coal Industry Teachers College,Anhui Biology Key Laboratory of Resource Plant,Huaibei,Anhui 235000);Studies on Bioactivity of Extracts from Pinellia ternata (Thunb.) Breit on Myzus persicae(Sulzer)[J];Journal of Anhui Agricultural Sciences;2009-09
9 ZHAO Hong-yan et al(Department of Life Sciences and Technology,Xinxiang University,Xinxiang,Henan 453000);Changes of Eucommia ulmoides Leaf in Organization Structure and Index Component Contents during Its Growth and Development Process[J];Journal of Anhui Agricultural Sciences;2009-14
10 XING Bing-cong et al(College of Forestry and Biotechnology,Zhejiang Forestry University,Hangzhou,Zhejiang 311300);Progress in Curcumin Derivatives form Curcuma wenyujin[J];Journal of Anhui Agricultural Sciences;2009-16
China Proceedings of conference Full-text Database 10 Hits
1 REN Hong-yan~1 SUN Xia~(1,2) ZHENG Cheng-shu~(1,2) SHU Huai-rui~(1,2,3) (1 College of Horticulture Science and Engineering,Shandong Agricultural University,Tai'an 271018,;2 College of Horticulture Science and Engineering,Shandong Agricultural University/State Key Laboratory of Crop Biology,Tai'an 271018; 3 Shandong Agricultural University National Apple Engineering Technique Center,Tai'an 271018);Differential Analysis of Flowering Related Genes by cDNA-AFLP in Chrysanthemum[A];[C];2011
2 REN Hong-yan CHENG Kai ZHENG Cheng-shu (College of Horticulture Science and Engineering,Shandong Agricultural University /State Key Laboratory of Crop B iology,Tai'an 271018);Establishment and Application of cDNA-AFLP Analysis System of Chrysanthemum[A];[C];2011
3 Peiguang Pan The Affiliated Nanhai Maternity and Children Hospital of Guangzhou Traditional Chinese Medicine University;Foshan;Guangdong.China.528200;Magnolia powder applicated to fart after abdominal surgery for a multi-center,randomized,open,parallel clinical study[A];[C];2012
4 PENG Bo1, 2 HU Xing-Ming2 DENG Wen2 YE Chu-Hua2 ( 1Wuhan University of College of Life Sciences, Wuhan 430072, China; 2 Industrial Crops Institute of Hubei Academy of Agricultural Sciences, Wuhan 430064, China);Correlation Analysis of SSR Markers and Phenotypic Trait in Mulberry[A];[C];2009
5 PENG Bo12 HU Xing-Ming2 YE Chu-Hua2 DENG Wen2 (1Wuhan University of College of Life Sciences, Wuhan 430072, China; 2 Industrial Crops Institute of Hubei Academy of Agricultural Sciences, Wuhan 430064, China);Genetic Diversity Analysis of Mulberry Germplasm Resources Based on SSR Markers[A];[C];2009
6 LI Jia-min,LI Hai-yan,LI Jing-xiao,WANG Xin-xing,WANG Wen-guang,SUN Xiao-mei,DAIJie-jie (Institute of medical Biology,Chinese Academy of Medical Science &Peking Union Medical College,Kunming 650118, China);Characterization and genetic diversity analysis of microsatellite DNA in Tree Shrews(Tupaia Belangeri Chinensis)[A];[C];2010
7 Zhang Chunhui,Wu Aiying,Wang Youlan (Qingdao Institute for Drug Control,Shandong Province,Qingdao 266071);Identification on Radix Puerariae thomsonii and its adulterant Radix Puerariae edulis[A];[C];2008
8 Lu Xiao-xia~1,Jiang Li-jia~1,Fan Jing~1,Wang Duo-qin~1,Tang Min~1,Xia Lei~1,Ma Ling-di~(1,*) 1 Changzhou Second Hospital,Nanjing Medical University,Changzhou 213003;2 The first clinical college,Nanjing Medical University,Nanjing 210029;Effect of matrine on proliferation inhibition and apoptosis induction in K562 cells in vitro[A];[C];2009
9 Wu Lihong,Wang Zhengtao (Shanghai R&D Center for Standardization of Chinese Medicines,Shanghai 201210,China; The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201210,China);Inheritance of Chinese Gentian Root[A];[C];2010
10 ;Analysis on cultivation influence on Genetic Diversity of authentic Glycyrrhiza uralensis[A];[C];2010
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 LIANG Li-jian,JIANG Yu-mei,XIA Bing,JIANG Ming-min, PENG Feng,HE Shu-lan,WANG Ren*(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China);Cloning and Prokaryotic Expression of LrCOMT from Lycoris radiata[J];Acta Botanica Boreali-Occidentalia Sinica;2012-01
2 TANG Jin-feng,LU Yao-bang*,GUI Liu-zi(Pharmaceutical College,TCM University of Hunan,Changsha,Hunan 410208,China);Comparison of total RNA extraction between Lycoris aurea Herb Flowers and scapes[J];Journal of Traditional Chinese Medicine University of Hunan;2012-01
3 ZHENG Ying1,LIU Xin2,DING Li-sheng21Chengdu Library of Chinese Academy of Sciences,Chengdu 610041,China;2Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China;Progress on Phytochemical Research for Benzylphenethylamine Alkaloids[J];Natural Product Research and Development;2009-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LI Yan,WANG Tie-mei,LU Xin-shi(Grassland Resources and Ecology Laboretry,Beijing Forestry University,Beijing 100083,China);Comparative study on extraction methods of total RNA from creeeping-rooted alfalfa[J];Pratacultural Science;2010-05
2 ZHANG Qian~1,LIN Shan-zhi~1,LIN Yuan-zhen~1,ZHANG Zhi-yi~(1*),WANG Zeliang~1,YANG Jun-jie~2(1 Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants,Mninstry of Education,Beijing Forestry University,Beijing 100083,China;2 Inner Mongolia Baotou Forest Industry Bureau,Baotou,Inner Mongolia 014000,China);Research Advances of Resistant Genes in Forest Trees[J];Acta Botanica Boreali-occidentalia Sinica;2005-09
3 YUAN Ju-hong1,2,PENG Feng2,FENG Xu2,SUN Shi2,HE Shu-lan2,XIA Bing1,2(1 College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2 Institute of Botany,Jiangsu Province & Chinese Academy of Sciences,Nanjing Botanical Garden (Mem.Sun Yat-sen),Nanjing 210014,China);Inter-specific Relationships Analysis and Classification of Lycoris by HPLC Chromatograms[J];Acta Botanica Boreali-Occidentalia Sinica;2007-11
4 Lu Gaolian and Hu Zhenghai(Institute of Botany,Northwestern University,Xi’an 710069);HISTOCHEMISTRY OF BERBERINE OF COPTIS CHINENSIS FRANCH.RHIZOME[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1994-03
5 WU Yan-Hua1,RUAN Wen-ke2,PENG Li-ying1(1.College of Veterinary Medicine,China Agricultural University,Beijing,100094,China;2.Beijing Agricultural College,Beijing,102206,China);Progress on Nanotechnology in Biomedicine[J];Progress in Veterinary Medicine;2007-S1
6 WT4”BX]BAO Jin Ku, WU Chuan fang,LU Hui,ZHOU Hong, ZENG Zhong kui, XU Pin long, ZOU Jian ( College of Life Science, Sichuan University, Chengdu 610064, China);THE EFFECT OF METAL ION AND DENATURANT ON CONFORMATION AND FUNCTION OF POLYGONATUM CYRTONEMA HUA. LECTIN Ⅱ[J];Journal of Sichuan University (Natural Science Edition);2000-06
7 SUN Jian-Zhong and WANG Ke-Yi(Shanghai Institute of Biochemistry,Academia Sinica,200031);Study of Trichosanthes kirilowii Lectin by Fluorescence Spectroscopy[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1994-06
8 WANG ShouYe, YU HuaMing, CHEN ZhenLong, ZHANG ZuDe, WU ShuangDing and LIU QingLiang*( Department of Chemistry, University of Science and Technology of China, Hefei 230026, China) ;Fluorescence Analysis of the Fibrinolytic Principle(FP) from Agkistrodon acutus Venom[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1998-03
9 Jia Jianhang\ Wang Bin (Institute of Genetics,Chinese Academy of Sciences,Beijing 100101);Recent advances in cloning of plant disease resistant gene[J];PROGRESS IN BIOTECHNOLOGY;2000-01
10 Ruan, Kang-cheng; Shi, Wei-xing; Zheng, Le; Sun, Ce(Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031);A Study of Phaseolus coccineus var. Rubronanus Lectin by Fluorescence Spectroscopy[J];;1994-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved