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《Marine Fisheries》 2013-02
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EST-SSR analysis and development of Paralichthys olivaceus based on public database

LIU Yi1,2,CHEN Wei-xing1,ZHOU Dan1,CHEN Xiao-ting1, WANG Mei-yu1,WANG Gui-xing2,LIU Hai-jin3(1.College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China; 2.Beidaihe Central Experiment Station,Chinese Academy of Fishery Sciences,Qinhuangdao 066100,China; 3.Chinese Academy of Fishery Sciences,Beijing 100141,China)  
In order to make the most of public database and provide new molecular markers for genetic research in Paralichthys olivaceus,15 268 ESTs of Paralichthys olivaceus from GenBank were analyzed.A total of 917 ESTs contained SSR with a frequency of 12.6% and a density of 1/5.7 kb.The dominant repeat motifs were dinucleotide and trinucleotide(which were 63.5% and 24.3% respectively).In dinucleotide repeat motifs,(AC)n was the most abundant repeat type(63.9%) while(AGC)n was the most abundant in trinucleotide(15%).The predominant motif repeat times was six,and the frequency was 24.0%.A total of 357 pairs of primers were found to be effective in a Paralichthys olivaceus population with a frequency of 73.8%.The results could provid the basis for developing EST-SSR from public EST databases in Paralichthys olivaceus and some allied species.And it could also provide new molecular markers for genetic research.
【Fund】: 国家863高技术研究发展计划(2012AA10A408-05);; 国家鲆鲽类产业技术体系专项(CARS-50-G2)
【CateGory Index】: S917.4
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 CHEN Songbo1,GONG Li1,LIU Haijin2(1.College of Animal Sciences and Technology,Northeast Agricultural University,Harbin 150030,China;2.Chinese Academy of Fisheries Sciences,Beijing 100039,China);Analysis of SSR information in EST resources of Japanese flounder (Paralichthys olivaceus)[J];Journal of Northeast Agricultural University;2010-10
2 SHEN Wang,YE Mao,SHI Ge,WANG Ri-xin(Laboratory of Marine Biology Resources and Molecular Engineering,Ocean Science College,Zhejiang Ocean University,Zhoushan 316004,China);Construction of a full-length cDNA library of Portunus trituberculatus hemocytes and analysis of partial expressed sequence tags[J];Marine Fisheries;2010-01
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【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 JIANG Shui-heng1,XU Shan-liang2,SHAO Bo1,HUA Yu-ming1,WEI Xiao-rong1,ZHANG Ke-sheng1(1.School of Marine Science,Ningbo University,Ningbo 315211,China; 2.Key Laboratory of Marine Biotechnology of Zhejiang,Ningbo 315211,China);Comparative studies on digestive enzymes and SOD enzyme between wild and cultured Portunus trituberculatus[J];Marine Fisheries;2012-02
2 XU Xiao-jun,ZHANG Hai-qi,ZHANG Chao,HE Zhong-yang(Zhejiang Fishery Technology Extention Station,Hangzhou 310012,China);Analysis of Simple Sequence Repeats(SSR) Information in Expressed Sequence Tag(EST) Resources of Pelodiscus sinensis[J];Journal of Economic Animal;2013-01
3 CAI Xiao-Hui, PENG Yin-Hui, ZHAO Peng, SONG Zhong-Kui, ZHANG Qin, HUANG Guo-Qiang (Guangxi Institute of Oceanology, Key Laboratory of Marine Biotechnology of Guangxi, Beihai, 536000);CONSTRUCTION OF HAEMOLYMPH cDNA LIBRARY OF SCYLLA PARAMAMOSAIN INDUCED BY VIBRIO PARAHAEMOLYTICUS AND ANALYSIS OF EXPRESSED SEQUENCE TAGs[J];Oceanologia et Limnologia Sinica;2013-03
4 JIANG Xia-min,WANG Jia,XUE Liang-yi,ZENG Jian-gang(College of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China);Construction of cDNA library from muscle tissue of Sepiella maindroni and analysis of expressed sequence tags[J];Journal of Oceanography in Taiwan Strait;2011-03
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【Secondary Citations】
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