Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Marine Fisheries》 2013-02
Add to Favorite Get Latest Update

EST-SSR analysis and development of Paralichthys olivaceus based on public database

LIU Yi1,2,CHEN Wei-xing1,ZHOU Dan1,CHEN Xiao-ting1, WANG Mei-yu1,WANG Gui-xing2,LIU Hai-jin3(1.College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China; 2.Beidaihe Central Experiment Station,Chinese Academy of Fishery Sciences,Qinhuangdao 066100,China; 3.Chinese Academy of Fishery Sciences,Beijing 100141,China)  
In order to make the most of public database and provide new molecular markers for genetic research in Paralichthys olivaceus,15 268 ESTs of Paralichthys olivaceus from GenBank were analyzed.A total of 917 ESTs contained SSR with a frequency of 12.6% and a density of 1/5.7 kb.The dominant repeat motifs were dinucleotide and trinucleotide(which were 63.5% and 24.3% respectively).In dinucleotide repeat motifs,(AC)n was the most abundant repeat type(63.9%) while(AGC)n was the most abundant in trinucleotide(15%).The predominant motif repeat times was six,and the frequency was 24.0%.A total of 357 pairs of primers were found to be effective in a Paralichthys olivaceus population with a frequency of 73.8%.The results could provid the basis for developing EST-SSR from public EST databases in Paralichthys olivaceus and some allied species.And it could also provide new molecular markers for genetic research.
【Fund】: 国家863高技术研究发展计划(2012AA10A408-05);; 国家鲆鲽类产业技术体系专项(CARS-50-G2)
【CateGory Index】: S917.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 CHEN Songbo1,GONG Li1,LIU Haijin2(1.College of Animal Sciences and Technology,Northeast Agricultural University,Harbin 150030,China;2.Chinese Academy of Fisheries Sciences,Beijing 100039,China);Analysis of SSR information in EST resources of Japanese flounder (Paralichthys olivaceus)[J];Journal of Northeast Agricultural University;2010-10
2 SHEN Wang,YE Mao,SHI Ge,WANG Ri-xin(Laboratory of Marine Biology Resources and Molecular Engineering,Ocean Science College,Zhejiang Ocean University,Zhoushan 316004,China);Construction of a full-length cDNA library of Portunus trituberculatus hemocytes and analysis of partial expressed sequence tags[J];Marine Fisheries;2010-01
3 SUN Dian-Qiao,SUN Yue-Na,WANG Ri-Xin and XU Tian-Jun(Laboratory for Marine Living Resources and Molecular Engineering,College of Marine Science,Zhejiang Ocean University,Zhoushan 316000,China);THE CHARACTERISTIC ANALYSIS OF MICROSATELLITES FROM ESTS IN MIICHTHYS MIIUY[J];Acta Hydrobiologica Sinica;2011-05
Chinese Journal Full-text Database 5 Hits
1 JIANG Shui-heng1,XU Shan-liang2,SHAO Bo1,HUA Yu-ming1,WEI Xiao-rong1,ZHANG Ke-sheng1(1.School of Marine Science,Ningbo University,Ningbo 315211,China; 2.Key Laboratory of Marine Biotechnology of Zhejiang,Ningbo 315211,China);Comparative studies on digestive enzymes and SOD enzyme between wild and cultured Portunus trituberculatus[J];Marine Fisheries;2012-02
2 XU Xiao-jun,ZHANG Hai-qi,ZHANG Chao,HE Zhong-yang(Zhejiang Fishery Technology Extention Station,Hangzhou 310012,China);Analysis of Simple Sequence Repeats(SSR) Information in Expressed Sequence Tag(EST) Resources of Pelodiscus sinensis[J];Journal of Economic Animal;2013-01
3 CAI Xiao-Hui, PENG Yin-Hui, ZHAO Peng, SONG Zhong-Kui, ZHANG Qin, HUANG Guo-Qiang (Guangxi Institute of Oceanology, Key Laboratory of Marine Biotechnology of Guangxi, Beihai, 536000);CONSTRUCTION OF HAEMOLYMPH cDNA LIBRARY OF SCYLLA PARAMAMOSAIN INDUCED BY VIBRIO PARAHAEMOLYTICUS AND ANALYSIS OF EXPRESSED SEQUENCE TAGs[J];Oceanologia et Limnologia Sinica;2013-03
4 JIANG Xia-min,WANG Jia,XUE Liang-yi,ZENG Jian-gang(College of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China);Construction of cDNA library from muscle tissue of Sepiella maindroni and analysis of expressed sequence tags[J];Journal of Oceanography in Taiwan Strait;2011-03
5 ZENG Cong;GAO Ze-Xia;LUO Wei;LIU Xiao-Lian;WANG Wei-Min;Key Lab. of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Key Lab. of Freshwater Animal Breeding, Ministry of Agriculture, College of Fishery, Huazhong Agricultural University;Freshwater Aquaculture Collaborative Innovation Center of Hubei Province;;CHARACTERISTICS OF MICROSATELLITES IN BLUNT SNOUT BREAM (MEGALOBRAMA AMBLYCEPHALA) EST SEQUENCES USING 454 FLX[J];Acta Hydrobiologica Sinica;2013-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Hui-qunJIN ShanWANG Guo-liangXIE Jue-xiao (Faculty of Life Science and Biotechology of Ningbo University, Ningbo 315211, China);Hemocytes and Blood Biochemical Values in Portunus trituberculatus[J];Fisheries Science;2004-06
2 LI Chang-hong,JIN Shan(College of Life Science and Biotechnology,Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo 315211,China);Preliminary Study on Immune Function of the Haemolymph in Crab Portunus trituberculatus[J];Fisheries Science;2008-04
3 SHENG Yunyan,LUAN Feishi,CHEN Kenong(Horticulture College,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Genetic diversity of Chinese melon cultivars(Cucumis melo L.) based on simple sequence repeat markers[J];Journal of Northeast Agricultural University;2006-02
4 YANG Jing1.2,LIU Haiying2,QIAN Chunrong3,JIN Zhenxun2(1.Harbin Academy of Agricultural Sciences,Harbin 150070,China; 2.College of Agriculture,Northeast Agricultural University,Harbin 150030,China; 3.Crop Tillage and Cultivation Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China);Analysis on genetic diversity of rice varieties in Heilongjiang Province by using SSR[J];Journal of Northeast Agricultural University;2008-06
5 LIANG Qing-long~1,DAI Cong-jie~2,CHEN Yin-shan~3(1.Quanzhou Health school, Quanzhou 362000, China;2.Biology Department Quanzhou Normal College, Quanzhou 362000, China;3.Bioengineering College ,Fujian Normal University, Fuzhou 350007, China);Comparison on Agglutinating Activity of Serum Lectins Extract from Three Species of Crabs[J];Journal of Fujian Fisheries;2005-02
6 Li Honglei, Song Linsheng, Wang Lingling, Xu Wei, Xiang Jianhai (Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071);Screening Microsatellite Markers from EST Sequence of Chlamys farreri[J];High Technology Letters;2003-12
7 HOU Zhan-Hui, WANG Yan, SHI Yao-Hua, GU Zhi-Feng, WANG Ai-Min (Key Laboratory for Tropical Biology Resources (MOE); Ocean College, Hainan University; Hainan Key Laboratory of Tropical Hydrobiology Technology, Haikou, 570228);EST-SSR ANALYSIS OF GENETIC VARIATION BETWEEN TWO GEOGRAPHICAL POPULATIONS OF PEARL OYSTER, PINCTADA MARTENSII (DUNKER)[J];Oceanologia Et Limnologia Sinica;2008-02
8 SHAN Xiu-Juan, DOU Shuo-Zeng (Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071);ALLOMETRIC GROWTH OF CROAKER MIICHTHYS MIIUY LARVAE AND JUVENILES AND ITS ECOLOGICAL IMPLICATION[J];Oceanologia Et Limnologia Sinica;2009-06
9 SHAN Le-zhou,XIE Qi-lang,SHAO Xin-bin,YAN Mao-cang(Zhejiang Mariculture Reseach Institute,Wenzhou 325005,China);Study on embryonic development and morphological characteristic habitual behavior for larvae,juvenile and young of Miichthys Miiuy[J];Marine Sciences;2010-01
10 LIN Fan-xue1, 2, CHENG Shui-ming2, LI An-zheng1, LIN Fang-can1** (1. Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070, China; 2. Department of Biology, Jining Teachers School, Jining 272125, China; 3. Department of Biology, Huanggang Normal University , Huanggang 438000, China);Selection of EST-SSR Primers for Lentinula edodes[J];Journal of Agricultural Biotechnology;2007-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved