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《Chemical Analysis and Meterage》 2011-03
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DETERMINATION OF SIX ANABOLIC-ANDROGENIC STEROIDS IN BEEF MUSCLE TISSUES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Yi Wenwen1,Quan Can2,Jin Junsu1 (1.College of Chemical Engineering,Beijing University of Chemical Technology,Beijing 100029,China;2.Division of Chemistry,National Institute of Metrology,Beijing 100013,China)  
A HPLC method was established for the simultaneous determination of six anabolic-androgenic steroids including methandienone,nandrolone,boldenone,methyltestosterone,testosterone propionate and nantrolone propionate in beef muscle tissues.The samples were extracted with acetonitrile and then cleaned up by freezing-lipid filtration.The mobile phase consisted of methanol-water at flow rate of 1.0 mL/min.The detection limits for the method were 0.005 1-0.008 6 mg/kg,and the limits of quantification were 0.022 0-0.028 7 mg/kg.The linear calibration curves were obtained in the concentration range of 0.25-10 mg/L,with r2 more than 0.999.The recoveries of the six compounds were 84.1%-100.0% fortified at the 0.4 mg/kg and 2.0 mg/kg levels,and the relative standard deviations of determination results were 0.5%-3.5%(n=5).The established method is simple,rapid,sensitive and specific,and it is appropriate for the identification and quantification of anabolic androgenic steroids in animal muscle tissues.
【Key Words】: high performance liquid chromatography anabolic androgenic steroid beef
【Fund】: 科技部国家科技支撑项目(2009BAK61B02) 科技部创新方法专项(2008IM021900);; 质检总局食品安全专项(21-ASPAQ1101) 质检总局食品安全专项(ASPAQ0809-1009)
【CateGory Index】: O657.72;TS251.52
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 Qin Yan, Chen Jie, Zhang Meijin(Food Inspection Laboratory of Guangzhou Entry-Exit Inspection & Quarantine Burean, Guangzhou 510623);Determination of Anabolic Hormones Multi-residues in Animal Muscle Tissues Using Liquid Chromatography-Tandem Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2006-03
2 HE Limin1,HUANG Xianhui1,FANG Binghu1,HUANG Shixin2,CAO Ying2,CHEN Jianxin1,ZENG Zhenling1,CHEN Zhangliu1(1.Department of Pharmacology and Toxicology,College of Veterinary Medicine,South China Agriculture University,Guangzhou 510642,China;2.Shanghai Institute for Veterinary Drug and Feedstuffs Control,Shanghai 201103,China);Determination of eleven steroid hormones in animal muscle tissues and eggs using ultra-performance liquid chromatography-tandem mass spectrometry[J];Chinese Journal of Chromatography;2008-06
3 XU Hong,LIN An-qing,GU Long,WU Yan-hui,HE Jia,ZHANG Man,ZHANG Jun,TANG Dan-zhou (Tianjin Inspection and Quarantine Bureau,Tianjin 300201,China);Determination of 8 Anabolic Hormones and Metabolites Residues in Animal Tissue by Liquid Chromatography-Tandem Mass Spectrometry Method[J];Journal of Instrumental Analysis;2008-12
4 ZHANG Aizhi 1,WANG Quanlin1,SHEN Jian1,ZHANG Shufen1,CHEN Liren2(1.Ningbo Academy of Product Quality Supervision & Inspection,Ningbo 315041,China;2.Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China);Simultaneous determination of seven sex hormones in fish products using ultra performance liquid chromatography-tandem mass spectrometry[J];Chinese Journal of Chromatography;2010-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Yan Hui1, Xiang Ping2, Shen Bao-Hua2, Wang Meng-Ye1, Shen Min2 1(Forensic Medicine Department of Shanghai Medical College, Fudan University, Shanghai 200032) 2(Institude of Forensic Sciences, Minisitry of Justice, Shanghai 200063);Analysis of 10 Kinds of Anabolic Androgenic Steroids in Human Hair by High Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2007-07
2 Zou Long, Lin Hong , Jiang Jie (College of Food Science and Engineering, Ocean University of China, Qingdao 266003);Determination of β-Estradiol Residues in Fish/Shellfish Muscle Tissues by Gas Chromatography-Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2007-07
3 SUN Han-Wen 1,KANG Zhan-Sheng1,2,LI Hui1,2 1(College of Chemistry and Environmental Science,Hebei University,Key Laboratory of Analytical Science of Hebei Province,Baoding 071002) 2(Hebei Institute of Food Quality Supervision Inspection and Research,Shijiazhuang 050051);Determination of Nine Steroid Hormones Residues in Beef Samples by Gel Permeation Chromatography-Solid Phase Extraction-Rapid Resolution Liquid Chromatography-Tandem Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2010-09
4 WANG Peng,WANG Fu-rong,LUO Jing,LI Jing,ZHAO Xiao-ya, FU Xiao-fang,GUO Shao-fei,SHANG Yin-zhu,SUN Ming-jun(Hubei Enter-Exit Inspection and Quarantine Bureau,Wuhan 430050);Determination of Spiroxamine Residues in Foodstuffs of Animal Origin by Gas Chromatography-mass Spectrometry[J];Journal of Analytical Science;2011-02
5 Dan De-zhong,LENG Geng and HUANG Pu-xin(Department of Environmental Science and Engineering,Sichuan University,Chengdu 610065);Analysis of environmental samples[J];Chinese Journal of Analysis Laboratory;2010-07
6 Wang Jianhua1, Pang Guofang2, Ding Ning3, Li Jie1 (1.Shandong Exit & Entry Inspection and Quarantine Bureau, Qingdao 266002, China; 2.Qinhuangdao Exit & Entry Inspection and Quarantine Bureau, Qinghuangdao 066002, China; 3.Shandong Academy of Medical Sceiences, Jinan 250061, China);DETERMINATION OF ESTRADIOL IN BOVINE URINE BY GAS CHROMATOGRAPHY-NEGATIVE-ION CHEMICAL IONIZATION MASS SPECTROMETRY[J];Chemical Analysis and Meterage;2007-03
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【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Qin Yan, Chen Jie, Zhang Meijin(Food Inspection Laboratory of Guangzhou Entry-Exit Inspection & Quarantine Burean, Guangzhou 510623);Determination of Anabolic Hormones Multi-residues in Animal Muscle Tissues Using Liquid Chromatography-Tandem Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2006-03
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3 XU Hong1,PANG Guo-fang2,LIN An-qing1,GU Long1,HE Jia1,ZHANG Man1,ZHANG Jun1,LIN Na1(1.Tianjin Inspection and Quarantine Bureau,Tianjin 300201,China;2.QinhuangdaoInspection and Quarantine Bureau,Qinhuangdao 066002,China);Simultaneous Determination of Residual Nonsteroid Anabolic Hormones in Animal Tissues by Liquid Chromatography-Tandem Mass Spectrometry[J];Journal of Instrumental Analysis;2007-06
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