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《West China Journal of Stomatology》 2002-01
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The Biological Effects of Recombinant Human Bone Morphogenetic Protein 2 on Human Periodontal Ligament Fibroblasts

Si Xiaohui, Liu Zheng The Ninth People′s Hospital, Shanghai Second Medical University  
Objective:The purpose of this study is to study the biological effects of recombinant human bone morphogenetic protein 2 (rhBMP2) on human periodontal ligament fibroblasts (HPDLFs).Methods: Human periodontal ligament fibroblasts were primary cultured and detected the different doses of rhBMP2 on their proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and formation of the mineralized nodules.Results: rhBMP2 (0 25~2 mg/ml) had no remarkable effect on the proliferation of HPDLFs. The ALP activity, OC synthesis and formation of the mineralized nodules of HPDLFs were significantly stimulated by 0 5~2 mg/ml rhBMP2.Conclusion: The effects of rhBMP2 on HPDLFs are dose_dependent. Not only can rhBMP2 stimulate the expression but also promote the maturation of osteoblastic phenotype of HPDLFs.
【Fund】: 国家自然科学基金资助项目 (编号 3 0 0 0 0 191)
【CateGory Index】: R780.2
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