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《West China Journal of Stomatology》 2003-05
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Bone Induction by Human Periodontal Ligament Fibroblasts Transfected with Bone Morphogenetic Protein 2 Gene

SI Xiaohui, LIU Zheng. (Research Institute of Stomatology, The Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China)  
Objective\ To establish human periodontal ligament fibroblasts (HPDLFs) that express bone morphogenetic protein 2 and observe their osteogenesis in vivo, so as to provide fundamental data for future study on gene therapy of periodontal defect treatment.Methods\ The phagemid expression vector for BMP_2 (pBK_B2) was transfected into HPDLFs by using Lipofect AMINE. The BMP_2 expression was determined by immunohistochemical ABC method. The BMP_2 gene transfected HPDLFs were injected into thigh muscle of nude mice under sterile condition. Specimens were collected in 21 days after injection and underwent normal procedures for fixation, decalcification, embedding, incising and staining with HE.Results\ The results demonstrated that BMP_2 protein expressed in HPDLFs after BMP_2 gene transfection. The HPDLFs transfected with BMP_2 gene could induce new bone formation at the injection site.Conclusion\ The results indicated that BMP_2 gene was transfected successfully and expressed efficiently in HPDLFs. The HPDLFs transfected with BMP_2 gene are capable of inducing new bone formation in vivo.
【Fund】: 国家自然科学基金 (编号 30 0 0 0 1 91 );; 中国博士后科学基金 (编号中博基 1 999第 1 7号 );; 上海市博士后基金 (编号沪科 2 0 0 0第 487号 )资助项目
【CateGory Index】: R781.4
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