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《West China Journal of Stomatology》 2003-05
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Evaluation of in vitro Cario-static Effect of Galla chinensis with Biofilm Model

LI Jiyao\+*, HUANG Zhengwei, ZHOU Xuedong, et al. (\+*Key Lab of Oral Biomedical Engineering Sichuan University, Ministry of Education, Chengdu 610041, China)  
Objective\ To evaluate the in vitro cario_static effect of Galla chinensis with biofilm model. Methods\ A four_organism bacterial consortium was cultured in a biofilm model on hydroxyapatite (HA) discs in a continuous culture system and exposed to repeated solution pulsing. There were three groups with different solution pulsed in the model: negative control group was pulsed with distilled water, positive control group was pulsed with 100 mmol/L sucrose solution and experimental group was pulsed with 100 mmol/L sucrose solution containing 4 0 g/L Galla chinensis. During the experiment, the dynamic changes of pH were recorded. After 6 pulses, surface structure of the biofilm was observed with a scanning electron microscope and the population on the biofilm was enumerated.Results\ Galla chinensis significantly inhibited the adherence of Actinomyces naelundii to HA disc compared with the control group and facilitated the removal of acid products. It was also found that the extra_cellular polysaccharide was reduced with the pulsing of Galla chinensis.Conclusion\ Galla chinensis in the biofilm model can partially reduce the cario_genic response of sucrose solution.
【Fund】: 国家自然科学基金 (编号 30 1 71 1 75);; 四川省教育厅国际合作项目基金 (编号 41 30 2 0 )资助项目
【CateGory Index】: R285.5
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