Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《West China Journal of Stomatology》 2008-02
Add to Favorite Get Latest Update

Ultrasound-mediated microbubble destruction enhances exogenous gene expression in NIH3T3 cells in vitro

SUN Qin-feng1,LIU Yu2,YANG Pi-shan1,DU Fang1.(1.Dept.of Periodontology,School of Stomatology,Shandong University,Jinan 250012,China;2.Dept.of Periodontology,Stomatological Hospital,Medical College of Nanjing University,Nanjing 210008,China)  
Objective To investigate the transfection efficiency of the recombinant human bone morphogenetic protein-2(hBMP-2) gene in targeted cells by ultrasound-mediated microbubble destruction.Methods NIH3T3 cells' anabiosis was completed and went down to the 3rd or 4th generation,and cultured in 6 well plates.The cells were divided into 2 groups:Plasmid DNA and LipofectamineTM 2000 group(liposome group),plasmid DNA and ultrasound and microbubble group(ultrasound-mediated microbubble destruction group).Plasmid DNA was transfected into cells with liposome or ultrasound and microbubble.24-48 hours later,the transfection efficiency and the concentrations of hBMP-2 were measured with fluoresence microscope and enzyme-linked immunosorbent assay(ELISA) respectively.The data were analyzed by curve fitting and t-test of SPSS 11.5.Results The transfection efficiency rate was(7.30± 1.58)% in liposome group,compared with(11.77±3.16)% in ultrasound-mediated microbubble destruction group(P 0.05).The concentration of hBMP-2 after transfection was(1 164.35±724.67) pg/mL in liposome group,versus(2 932.70±656.27) pg/mL in ultrasound-mediated microbubble destruction group(P0.05).Conclusion Ultrasound-mediated microbubble destruction could significantly improve the transfection efficiency and expression of hBMP-2 gene in NIH3T3 cells.It may provide a new and effective gene delivery system for gene therapy in periodontal regeneration.
【Fund】: 山东省自然科学基金资助项目(Y2006C125)
【CateGory Index】: R346
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 SUN Qin-feng1, DU Fang2, XU Yan1, YANG Pi-shan1.(1. Dept. of Periodontology, School of Stomatology, Shandong University, Jinan 250012, China; 2. Dept. of Stomatology, Xuzhou Medical College, Xuzhou 221004, China);Ultrasound-mediated microbubble destruction enhances bone morphogenetic protein-2 gene expression in mouse skeletal muscles[J];West China Journal of Stomatology;2009-01
Chinese Journal Full-text Database 2 Hits
1 Si Xiaohui, Liu Zheng The Ninth People′s Hospital, Shanghai Second Medical University;The Biological Effects of Recombinant Human Bone Morphogenetic Protein 2 on Human Periodontal Ligament Fibroblasts[J];West China Journal of Stomatology;2002-01
2 GUAN Xiao-Xiang 1, FAN Le-Ming 1, CHEN Qi 1, LI Ming-Fang 1, LI Xiao-Rong 2, and SHAO Li-Zhen 2 (1. Atherosclerosis Research Center, Nanjing Medical University, Nanjing 210029, China. 2.Cardiovascular Division, Wuxi No.1 Hospital, Wuxi 214002,China);Ultrasound-Mediated Microbubble Destruction Enhances the Enhanced Green Fluorescence Protein C3 Gene Expression in Chinese Hamster Ovary Cells in Vitro[J];Chinese Journal of Arterial Lerosis;2003-01
Chinese Journal Full-text Database 5 Hits
1 ;The status guo of research and development trend of GTR materials[J];Chinese Journal of Dental Materials and Devices;2004-02
2 XIE Cheng-jie, WANG Yi-ning. School of Stomatology, Wuhan University, Wuhan 430079;Effects of Recombinant Bone Morphogenetic Protein-2 on Fibroblastic Phenotype of Periodontal Ligament Cells Population.[J];Journal of Comprehensive Stomatology;2005-04
3 Zhang Qunxia,Wang Zhigang,Ran Haitao,et al.Institute of Ultrasonic Image,Chongqing University of Medical Sciences, Chongqing 400010,China;Enhanced gene transfection in tumor cells by ultrasound and microbubbles[J];Journal of Clinical Ultrasound in Medicine;2005-02
4 SHI Jing-ping, WANG Jian-hua, LI Xiao-rong1, SHAO Li-zheng1, ZHANG Bin1, LU Chun2, WU Wen-xi*(Department of Surgery, the First Affiliated Hospital of NJMU, Nanjing 210029; 1Cardiovascular Division, Wuxi First Hospital Affiliated of NJMU, Wuxi 214002; 2Department of Microbiology and Immunology, NJMU, Nanjing 210029, China);Comparative study on efficiency of gene transfection mediated by ultrasound enhanced self-made albumin microbubble or SonoVue microbubble destruction in vitro[J];Acta Academiae Medicinae Nanjing;2005-06
5 WANG Jian-hua△, SHI Jing-ping, SHEN Li-zong, DING Qiang, LU Chun1, LIN Hong-jun2, WU Wen-xi(Department of General Surgery, the First Affiliated Hospital of NJMU, Nanjing 210029;1Department of Microbiology and Immunology, NJMU, Nanjing 210029; 2Department of Ultrasound, the First Affiliated Hospital of NJMU, Nanjing 210029, China);Ultrasound mediated sonoVue microbubble destruction enhances the reporter gene expression in liver in vivo[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2006-04
Chinese Journal Full-text Database 3 Hits
1 CHEN Yan-xiang1,QIAO Wei-hong1,LIU Dong-liang2,LI Zong-shi1(1.State Key Laboratory of Fine Chemicals,Dalian University of Technology,Dalian 116012,China;2.College of Chemistry and Chemical Engineering,Donghua University,Shanghai 201620,China);Targeted vectors of gene therapy[J];Journal of Dalian Medical University;2007-04
2 LIU Jian 1, HU Yun Yu 1, LIU Xin Ping 2, L Rong 1, MA Zhen Sheng 1, X Xin Zhi 1 1Institute of Orthopedics of Chinese PLA, Xijing Hospital, 2Department of Biochemistry & Molecular Biology, Faculty of Preclinical Medicine, Fourth Mili;Dynamic expression of BMP 1, 2, 3 genes during fracture healing[J];Journal of The Fourth Military Medical University;2001-22
3 DING Pei-hui, CHEN Li-li. (Dept. of Stomatology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China);Periodontal Regeneration by Gene Therapy[J];International Journal of Stomatology;2007-02
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Wang Qintao, Wu Zhifen, Dong Guangying, et al Department of Periodontology, College of Stomatology, the Fourth Military Medical University;Effects of BMP on Periodontal Ligament Cell's Cell Cycling and DNA Synthesis: a Flow Cytometry (FCM) Analysis[J];WEST CHINA JOURNAL FOF STOMATOLOGY;1999-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved