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《West China Journal of Stomatology》 2008-02
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Ultrasound-mediated microbubble destruction enhances exogenous gene expression in NIH3T3 cells in vitro

SUN Qin-feng1,LIU Yu2,YANG Pi-shan1,DU Fang1.(1.Dept.of Periodontology,School of Stomatology,Shandong University,Jinan 250012,China;2.Dept.of Periodontology,Stomatological Hospital,Medical College of Nanjing University,Nanjing 210008,China)  
Objective To investigate the transfection efficiency of the recombinant human bone morphogenetic protein-2(hBMP-2) gene in targeted cells by ultrasound-mediated microbubble destruction.Methods NIH3T3 cells' anabiosis was completed and went down to the 3rd or 4th generation,and cultured in 6 well plates.The cells were divided into 2 groups:Plasmid DNA and LipofectamineTM 2000 group(liposome group),plasmid DNA and ultrasound and microbubble group(ultrasound-mediated microbubble destruction group).Plasmid DNA was transfected into cells with liposome or ultrasound and microbubble.24-48 hours later,the transfection efficiency and the concentrations of hBMP-2 were measured with fluoresence microscope and enzyme-linked immunosorbent assay(ELISA) respectively.The data were analyzed by curve fitting and t-test of SPSS 11.5.Results The transfection efficiency rate was(7.30± 1.58)% in liposome group,compared with(11.77±3.16)% in ultrasound-mediated microbubble destruction group(P 0.05).The concentration of hBMP-2 after transfection was(1 164.35±724.67) pg/mL in liposome group,versus(2 932.70±656.27) pg/mL in ultrasound-mediated microbubble destruction group(P0.05).Conclusion Ultrasound-mediated microbubble destruction could significantly improve the transfection efficiency and expression of hBMP-2 gene in NIH3T3 cells.It may provide a new and effective gene delivery system for gene therapy in periodontal regeneration.
【Fund】: 山东省自然科学基金资助项目(Y2006C125)
【CateGory Index】: R346
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【References】
Chinese Journal Full-text Database 1 Hits
1 SUN Qin-feng1, DU Fang2, XU Yan1, YANG Pi-shan1.(1. Dept. of Periodontology, School of Stomatology, Shandong University, Jinan 250012, China; 2. Dept. of Stomatology, Xuzhou Medical College, Xuzhou 221004, China);Ultrasound-mediated microbubble destruction enhances bone morphogenetic protein-2 gene expression in mouse skeletal muscles[J];West China Journal of Stomatology;2009-01
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1 Si Xiaohui, Liu Zheng The Ninth People′s Hospital, Shanghai Second Medical University;The Biological Effects of Recombinant Human Bone Morphogenetic Protein 2 on Human Periodontal Ligament Fibroblasts[J];West China Journal of Stomatology;2002-01
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1 ;The status guo of research and development trend of GTR materials[J];Chinese Journal of Dental Materials and Devices;2004-02
2 XIE Cheng-jie, WANG Yi-ning. School of Stomatology, Wuhan University, Wuhan 430079;Effects of Recombinant Bone Morphogenetic Protein-2 on Fibroblastic Phenotype of Periodontal Ligament Cells Population.[J];Journal of Comprehensive Stomatology;2005-04
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1 CHEN Yan-xiang1,QIAO Wei-hong1,LIU Dong-liang2,LI Zong-shi1(1.State Key Laboratory of Fine Chemicals,Dalian University of Technology,Dalian 116012,China;2.College of Chemistry and Chemical Engineering,Donghua University,Shanghai 201620,China);Targeted vectors of gene therapy[J];Journal of Dalian Medical University;2007-04
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