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《Chemical World》 2006-06
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Cyto-compatible Evaluation of Carboxylm Ethyl Konjac Glucomannan-chitosan Blend Films

WANG Bi~1,YUE Xing-jian~1,QIN Song~1,ZHAO Bing~2(1.Department of Chemistry and Life Science,Neijiang Teachers College,Sichuan Neijiang 641112,China;2.Department of Orthopedics,West China Hospital,Sichuan University,Sichuan Chengdu,610041,China)  
Carboxylm ethyl konjac glucomannan-chitosan blend films were prepared successfully by solution method.The cell compatibility of the blend films was studied by cultivating fibroblasts NIH3T3 in vitro in blend film steep or on the surface of the blend film and hemolytic reaction.The MTT colorimetric assay result indicated that the blend film steep had no toxic effect to cell.Observed by microscope and scanning electron microscope,fibroblasts NIH3T3 which had been cultured 7 days stuck to the blend film well and grew vigorously.Comparing the growth of cell on porous and compact blend films,it was found that fibroblasts NIH3T3 could transfer into the interior of porous blend films.The heterolysis rate of blend film steep was smaller than 5%,so the konjac glucomannan-chitosan blend film was considered to have good cell compatibility.
【Fund】: 四川省应用基础项目(04JY029-022)
【CateGory Index】: R318.08
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