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《Acta Chimica Sinica》 2004-03
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Direct Electrochemistry of Horseradish Peroxidase at a Carbon Nanotube Electrode

CAI, Chen-Xin  CHEN, Jing (Department of Chemistry, Nanjing Normal University, Nanjing 210097)  
Horseradish peroxidase (HRP) immobilized on the surface of the carbon nanotube modified glassy carbon electrode (HRP-CNT/GC) can undergo effective and stable direct electron transfer reaction. The cyclic voltammetric results indicate that the HRP-CNT/GC electrode exhibits a pair of well-defined and nearly symmetrical redox peaks in 0.1 mol/L phosphate buffer solution. Its formal redox potential, E 0′, is almost independent on the scan rates, the average value of E 0′ is (-0.319±0.002) V (vs. SCE, pH 6.9) in the phosphate buffer solution (pH 6.9) in the scan rate range of 20~100 mV/s. The dependence of E 0′ on the pH of the buffer solution indicates that the direct electron transfer of HRP is a one-electron-transfer reaction process coupled with one-proton-transfer. The experimental results also demonstrated that the immobilized HRP retained its bioelectrocatalytic activity for the reduction of H 2O 2 and the HRP-CNT/GC electrode can rapidly respond to the change of the concentration of H 2O 2. The method presented here can be easily extended to obtain the direct electrochemistry of other redox enzymes or proteins.
【Fund】: 国家自然科学基金 (No .2 0 3730 2 7);; 教育部留学回国人员启动基金;; 江苏省高校自然科学基金 (No.0 3KJA1 50 0 55);; 江苏省南京市人事局回国人员择优项目资助课题
【CateGory Index】: O646.54
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