Radiolabeled Synthesis and Identification of a Novel Herbicide, Pro-pyl4-[2-(4,6-Dimethoxy-2-pyrimidinyloxy)benzylamino]benzoate with All Six Benzoic Ring Carbons Labeled by ~(14)C
YANG, Zheng-Mina,c YE, Qing-Fub TANG, Qing-Hongc LU, Long*,c CHEN, Zi-Yuanb (a College of Life Science, Northwest Science and Technology University of Agriculture and Forestry, Xianyang 712100) (b Institute of Nuclear-Agricultural Science, Zhejiang University, Hangzhou 310029) (c Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032)
A radiochemical, propyl 4-[2-(4,6-dimethoxy-2-pyrimidinyloxy)benzylamino]benzoate with all six benzoic ring carbons labeled by 14C was made from 4-aminobenzoic acid with all six benzoic ring car-bons labeled by 14C through esterification, condensation, reduction and substitution, and purified by prepara-tive HPLC. Its structure was identified by LC-MS and 1H NMR. HPLC test showed its chemical purity ex-ceeded 98%. HPLC-LSC and TLC-IIA demonstrated that its radiochemical purity exceeded 98% and spe-cific activity was 1.089±0.011 mCi/mmol. The total chemical yield and radiochemical yield were all 53%.