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《Acta Chimica Sinica》 2007-04
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Spectroscopic Investigation of the Binding of the Active Components of Apocynum venetum L. to Human Serum Albumin

SU,Zhong~(a,b) QIN,Chuan~a XIE,Meng-Xia~(*,a) LI,Jian-Dong~b WANG,Ying-Dian~a (~a Analytical & Testing Center of Beijing Normal University,Beijing 100875) (~b Institute of Grassland Sciences,Northeast Normal University,Changchun 130024)  
Quercetin (QUE),rutin (RUT) and catechin (CAT) are main active components of Apocynum venetum L.The binding mechanisms of these active components to human serum albumin (HSA) have been investigated utilizing fluorescence and UV absorption spectra.The results revealed that the fluorescence quenching arose mainly from static quenching by complex formation when the c_(QUE)/c_(HSA)≤3.5,and the pro- portion of dynamic quenching increased in higher drug concentration;while the quenching mechanism was mainly static in the drug concentration range studied for RUT.However,CAT cannot form complex with HSA.The binding site number was one for QUE and RUT,and the binding constants were (1.51±0.13)×10~5 and (0.81±0.08)×10~5 L·mol~(-1),respectively.The intrinsic fluorescence of QUE and RUT conspicuously enhanced in the presence of HSA due to excited-state proton transfer (ESPT) and it fur- ther confirmed the complex formation of HSA with QUE and RUT,individually.The UV absorption bands of QUE significantly red-shifted after interacting with HSA,which signified that the phenol group dissoci- ated during the QUE-protein binding process and the binding was driven by electrostatic force.However,the combination of RUT and HSA did not induce obvious red shift of UV absorption bands of RUT,and their binding force originated probably from the hydrogen bonding between RUT and HSA.Based on the second derivative UV absorption spectra,the binding modes of QUE and RUT were discussed.
【Fund】: 国家自然科学基金(No.30470180);; 新医药学科群(No.XK100270569);; 放射性药物教育部重点实验室开放基金.
【CateGory Index】: R914
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【References】
Chinese Journal Full-text Database 6 Hits
1 CHEN Yu-Hai ZHANG Yan-Qinga ZHAO Feng-Lia LIU Bao-Shenga(Baoding Vocational and Technical College,Baoding,Hebei 071051,P.R.China)a(Center of Physics and Chemistry,Hebei University,Baoding,Hebei 071002,P.R.China);Study on the Interaction between Levofloxacin and Human Serum Albumin by Fluorescence and Determination[J];Chinese Journal of Spectroscopy Laboratory;2008-02
2 LI,Lin-Wei WANG,Dong-Dong SUN,De-Zhi LIU,Min QU,Xiu-Kui (College of Chemistry and Chemical Engineering,Liaocheng University,Liaocheng 252059);Thermodynamic Study on Interaction between Anti-tumor Drug 5-Fluorouracil and Human Serum Albumin[J];Acta Chimica Sinica;2007-24
3 CAO Tuan-wu,YANG Ji-dong,CAI Si-bin(Department of Chemistry and Environment,Yangtze Normal University,Chongqing 408003,China);Interaction between Carbamazepine and bovine serum album[J];West China Journal of Pharmaceutical Sciences;2008-04
4 LING Xiao,BORJIHAN Ge-ri-le-tu,NA Ri-su,ZHAO Ri-ge-tu,WANG Yan-li,GUI Xiang (Institute of Macromolecule Chemistry and Mongolian Medicine,Inner Mongolia University,Hohhot 010021,China);Studies on Interaction between Piperine and Bovine Serum Albumin and Effects of Cu~(2+) and Fe~(3+) Ions on the Reaction[J];Journal of Instrumental Analysis;2008-10
5 CAO Tuan-Wu YANG Ji-Dong (Department of Chemistry and Environment, Yangtze Normal University, Chongqing 408100, P. R. China);Interaction between Malachite Green and Bovine Serum Albumin[J];Acta Physico-Chimica Sinica;2008-04
6 CHEN Jun-Jie YANG Mei ZHANG Lian-Ru* ZHENG Zhong-Hui SONG Si-Yang (School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China);Study on the Interaction of Gliotoxin with BSA[J];Microbiology;2009-08
【Citations】
Chinese Journal Full-text Database 3 Hits
1 XIE, Meng-Xia  JIANG, Min LI, Song LIU, Yuan (Analytical & Testing Center of Beijing Normal University, Beijing 100875);Study on the Interaction of β-1,2,3,4,6-Penta-O-galloyl-D-glucopyranose with Human Serum Albumin[J];Acta Chimica Sinica;2004-16
2 XIE, Meng-Xia* XU, Xiao-Yun WANG, Ying-Dian LIU, Yuan (Analytical and Testing Center, Beijing Normal University, Beijing 100875);Spectroscopic Investigation of the Interaction between 2,3-Dihydro- 4',5,7-trihydroxyflavone and Human Serum Albumin[J];Acta Chimica Sinica;2005-22
3 ZHOU Li,WANG Xiao-shan,HUANG He-ping,ZHOU Ya-qiu(Anhui College of Traditional Chinese Medicine,Hefei 230031,China);Determination of Total Flavonoids in Apocynum venetum L.from Different Regions[J];Research and Practice of Chinese Medicines;2005-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HAN Chao et al(College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025);Study on the Extraction and Stability of Yellow Calla's Pigment[J];Journal of Anhui Agricultural Sciences;2005-12
2 CHEN Chun-gang et al (College of Food Science,Henan Institute of Science and Technology,Xinxiang,Henan 453003);Research and Application of Bio-flavonoids[J];Journal of Anhui Agricultural Sciences;2006-13
3 REN Hui-li1,CAO Jun-mai2,CHEN Yan-yun1,LI Guo-qi1(1.Ningxia University,Ningxia,Yinchuan 750021,China;2.The Second Northwest University for Nationnalities,Ningxia,Yinchuan 750021,China);Current Research State and Exploitation of Apocynum venetum L.[J];Northern Horticulture;2008-07
4 Zeng Xiangqun Lin Shuchang (Department of Chemistry,Beijing Normal University,100875,Beijing);DETERMINATION OF TRACE COLCHINEOS BY SINGLE-SWEEP OSCILLOPOLAROGRAPHY[J];Journal of Beijing Normal University(Natural Science);1992-02
5 WANG Long\|cheng\+1, JI Zhen\|guo\+1, RUI Xiang\|xin\+1, YUAN Jun\+1, XIANG Yin\+1, WANG Mang\+1, Yasukiyo Ueda\+2 (1.State Key Laboratory for Silicon Materials,Zhejiang University,Hangzhou\ 310027,China; 2\^Faculty of Engineering Kobe University Rokk;UV-VIS and XPS Study of Lead Phthalocyanine and Phthalocyanine Composite Film Prepared by Vacuum Co-deposition Approach[J];Materials Science and Engineering;2002-02
6 JIANG Hong~(1,2) AN Pu-li~1 JIANG Ye~(1*) 1.Department of Analytical Chemistry,School of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China; 2.Equipment Center of Department of Training,Bethune Military Medical College,Shijiazhuang 050081;Drug and protein interactions:progress of research methods[J];Academic Journal of Second Military Medical University;2007-06
7 ZHAO Sheng-min,ZHENG Lai-jiuSchool of Textile & Light Industr,Dalian Polytechnic University,Dalian 116034,China;Study on technology of enzymatic degumming on apocynum[J];Journal of Dalian Polytechnic University;2008-03
8 Wang Lun;Zhu Changqing(Department of Chemistry, Anhui Normal University, Wuhu 241000);Determination of Benzene in Waste Water by Electrolytic Fluoresence Spectrophotometry[J];Chinese Journal of Analytieal Chemistry;1996-11
9 Liu Shumei 1,2, Li Junan *1, Mao Xun 1 1(College of Chemistry, Xiangtan University, Xiangtan 411105) 2 (Yueyang City Nitrogen Fertilizer Factory, Yueyang 414000);Determination of Zirconium by Second-order Derivative Adsorption Voltammetry of Zirconium C-Morin Complex at a Carbon Paste Electrode[J];Chinese Journal of Analytical Chemistry;2004-02
10 ZHENG Jian, CHEN Huan wen, LIU Hong wei, LI Bao hua, ZHANG Han qi, YU Ai min, JIN Qin han * (Changchun R&D Center for Analytical Instruments, Department of Chemistry, Jilin University, Changchun, 130023);Application of UV-Vis Spectrophotometry in Pharmaceuticals Analysis[J];Journal of Analytical Science;2002-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Yali\ WANG Haifang (Department of Technical Physics,Peking University,Beijing,100871);Interaction of Bovine Serum Albumin with Benzoate[J];Acta Scicentiarum Naturalum Universitis Pekinesis;2002-02
2 LIAN Chun-xia~1, GAN Ting-ting~1, CHEN Hua~1, YUAN Pei~2, XIA Zhi-ning~1(1.College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China;2. Pharmaceutical Department, Chongqing Medical University, Chongqing 400016, China);Determination of the Binding Constant Between Sodium Houttuyfonate and Human Serum Albumin by Capillary Electrophoresis[J];Journal of Chongqing University(Natural Science Edition);2004-02
3 Zheng Yongxi~* Li Longdi (Department of Chemistry,Tsinghua University,Beijing,100084);Micelle Solubilization (Ⅱ)[J];Chinese Journal of Analytical Chemistry;1990-03
4 Jiang Yunbao~*; Xu Jingou; Chen Guozhen(Department of Chemistry, Xiamen University, Xiamen 361005);A New Kind of Surfactant: Long-chain Alkyltriphenylphosphonium——Ⅵ. Determination of the Average Aggregate Number of Dodecyltriphenylphosphonium Bromide Micelle Based on Steady-state Fluorescence Quenching[J];Chinese Journal of Analytical Chemistry;1992-10
5 Meng Zhaohui , Wang Zhenhui , Zhou Shuping (Department of Chemistry, Henan Normal University, Xinxiang 453002);Determination of Piperine by Single-sweep Polarography[J];Chinese Journal of Analytieal Chemistry;1994-12
6 Feng Xizeng, Bai Chunli, Lin Zhang, Wang Naixin, Wang Chen (Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080);The Interaction between Acridine Orange and Bovine Serum Albumin[J];Chinese Journal of Analytieal Chemistry;1998-02
7 Xu Yan; Huang Hanguo (Department of Chemical Engineering, Hebei Institute of Science and Technology, Tangshan 063009) Shen Hanxi (Department of Chemistry, Nankai University, Tianjin 300071);Fluorescence Quenching Behavior of Human Serum Albumin by Quinolone Antibacterials[J];Chinese Journal of Analytieal Chemistry;1998-12
8 Gong Qiaojuan; Jin Weijun; Dong Chuan (Department of Chemistry,Shanxi university,Taiyuan 030006) (Yuncheng Advanced Training College ,Yuncheng 044000);Recognition and Determination of Ofloxacin Enantiomers by Synchronization-Derivative Fluorimetry[J];CHINESE JOURNAL OF ANALYTIEAL CHEMISTRY;2000-06
9 Wei Xiaofang;Liu Hnizhou (Young Scientist Laboratory of Separation Science and Engineering,Insritute of Chemical Metallurgy, Chinese Academy of Sciences,Beijing 100080);The Interaction Between Triton X-100 and Bovine Serum Albumin[J];CHINESE JOURNAL OF ANALYTIEAL CHEMISTRY;2000-06
10 Wu Shuqing, Cao Qiue, Zhao Yunkun, Zhang Wujuan, Chen Xingguo, Hu Zhide (Department of Chemistry, Lanzhou University, Lanzhou 730000) (Department of Chemistry, Yunnan University, Kunming 650091);The Fluorescence Characteristic of Th~(3+) -Ciprefloxacin and the Determination of Ciprofloxacin[J];Chinese Journal of Analytieal Chemistry;2000-12
【Secondary References】
Chinese Journal Full-text Database 6 Hits
1 CAO Tuan-wu﹡,YANG Ji-dong,WU Xing-fa,YI Xing-ming(Department of Chemistry and Environment,Yangtze Normal University,Chongqing 408003,China);Spectrophotometric study on the interaction between ranitidine and bovine serum album[J];Chemical Research and Application;2009-09
2 ZHANG Li,HAN Yong-jun,LIU Qiao-ru,ZHANG Qiu-xia,QIN De-zhi (College of Chemistry and Chemical Engineering,Pingdingshan University,Pingdingshan 467000,China);Study on the Microstructure of Protein in Soluble Complexes Containing Bovine Serum Albumin and Hg~(2+)[J];Journal of Instrumental Analysis;2009-04
3 ZONG Guo-Qiang1,2 LU Gong-Xuan1, (1State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, P. R. China; 2Graduate University of the Chinese Academy of Sciences, Beijing 100049, P. R. China);A NOR Fluorescent Logic Gate Based on N-(9-Anthracylmethyl)- L-histidine[J];Acta Physico-Chimica Sinica;2008-10
4 WU Jin-xiu,LI Mei,LIU Zhao-gang,HU Yan-hong(College of Rare Earth,Inner Mongolia University of Science and Technology,Baotou 014010,China);Advance in the Research on the Interaction of Rare Earth with Serum Albumins by Fluorescence Spectrum[J];Chinese Rare Earths;2009-02
5 ZHOU Can-song,YE Zhi-jun,LIU Ying-ju (Department of Applied Chemistry,College of Sciences,South China Agriculture University,Guangzhou 510642,China);HPLC investigation on the determination of apigenin and its interaction with human serum albumin[J];Chinese Journal of Pharmaceutical Analysis;2009-06
6 CHEN Jun-Jie YANG Mei ZHANG Lian-Ru* ZHENG Zhong-Hui SONG Si-Yang (School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China);Study on the Interaction of Gliotoxin with BSA[J];Microbiology;2009-08
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 XIE Meng-Xia *, LIU Yuan(Analytical & Testing Center, Beijing Normal University, Beijing 100875, China);Studies on Amide Ⅲ Infrared Bands for the Secondary Structure Determination of Proteins[J];Chemical Research In Chinese Universities;2003-02
2 LIU, Yuan XIE, Meng-Xia  KANG, Juan (Analytical & Testing Center, Beijing Normal University, Beijing 100875);Influence of Total Saponins of Panax notoginseng on the Conformation of BSA[J];Acta Chimica Sinica;2003-08
3 XIE, Meng-Xia  JIANG, Min LI, Song LIU, Yuan (Analytical & Testing Center of Beijing Normal University, Beijing 100875);Study on the Interaction of β-1,2,3,4,6-Penta-O-galloyl-D-glucopyranose with Human Serum Albumin[J];Acta Chimica Sinica;2004-16
4 Chen Dungliang;Nie Jiren;Zhu Ziqing;Study on the Chemical Components of the Roots of Apocynum Lancifolium Rus.Ⅰ、Isolation and Identification of the Liposoluble Components[J];Journal of Lanzhou University;1979-04
5 Yan Fu-sheng;Determination of Quercetin in the Extract of Bluish Dogbane by High—performance Lquid Chromtography[J];;1983-04
6 Peng Shuhua Department of Chemistry, Capital Institute of Medicine;The Determination of the Total Flavonoids Content in the Extract of Bluish Dogbane with Hypenin as a Standard[J];Journal of Capital University of Medical Sciences;1988-02
7 Yan Fusheng Department of Chemistry, Capital Institute of Medicine;On the Pharmacologic Activity of Bluish Dogbane Determination of the Trace Elements in the Extract[J];Journal of Capital University of Medical Sciences;1989-01
8 YAN XIUZHEN, MEI XINGGUO, LUAN XINHUI, QIAN XUESHE(Department of Chemistry of Natural Products, Faculty of Pharmacy, Shanghai First Medical College, Shanghai);STUDIES ON CONSTITUENTS OF STEMS OF APOCYNUM VENETUM LINN[J];;1985-04
9 Huang Xianglan, Zeng Fan Guangdong Provincial Institute of Materia Medica, Guangzhou 510180;Determination of Chemical Constituents of Luobumaye[J];ShiZhen Journal of Traditional Chinese Medicine Research;1998-06
10 MA Zhi hong,LU Zhong bing,SHI Bi ( The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University ,Chengdu 610065,China);CHEMICAL PROPERTIES AND APPLICATION OF TANNIC ACID[J];Natural Product Research and Development;2003-01
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