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《Acta Chimica Sinica》 2012-06
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Study on the Glutathione Molecularly Imprinted Polymers with Different Functional Monomers

Xin,Yu Tong,Yanjun Yang,Hailin Zhang,Ling Zhang,Yuran Chen,Yi Wang,Wu(Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology, Jiangnan University,Wuxi 214122)  
Using glutathione(GSH) as template molecule,acrylamide(AM) and methacrylic acid(MAA) as functional monomer,different glutathione-molecularly imprinted polymers(GSH-MIPs) were developed and synthesized with molecularly imprinted technique.The effects for adsorption capacity,including molar ratio of template molecule-functional monomer,pH of loading buffer,adsorption equilibrium time and loading concentration were discussed in detail.The result demonstrated that the polymers with raw material mole ratios at 1∶5 for GSH∶AM,and 1∶4 for GSH∶MAA showed higher adsorption capacity,the opti-mal pH values for the adsorption of GSH-MIPAMs and GSH-MIPMAAs to GSH were 3.0 and 5.0,respectively;furthermore,the optimal GSH loading concentrations were 1.50~2.00 g/L for GSH-MIPAMs,and 1.00~1.50 g/L for GSH-MIPMAAs;additionally,the adsorption equilibrium time was 20 h for GSH-MIPAMs and 18 h for GSH-MIPMAAs.In addition,the binding of GSH analogues were also applied to the synthesized poly-mers,and the results reflected that GSH-MIPs showed low recognition to these analogues.This protocol was further employed for the extraction and separation of GSH from yeast cells samples.The extraction recover-ies of GSH-MIPAMs and GSH-MIPMAAs were 41% and 77%,respectively,it was of great potential in the pu-rification of GSH.
【Fund】: 2010年江苏省科技支撑(No.SBE201077545);2011年江苏省科技支撑(No.SBE201170578);; 工业微生物重点实验室开放课题(No.KLIB-KF201005);; 江苏省高校优势学科建设工程资助项目;; “111”引智(No.111-2-06)资助项目~~
【CateGory Index】: O631.3
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