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《Chemical Research and Application》 2001-04
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STUDY OF GLUCOSE BIOSENSOR BASED ON PLATINIUM ELECTRODE MODIFIED BY PRUSSIAN BLUE

WANG Rong,GUO Xiao\|ming,WU Xia\|qin,ZHANG Zong\|rang (Dept. of Chemistry,Shanghai Teachers Univ.,Shanghai 200234,China)  
A glucose microbiosensor is reported.It is based on the modified electrode by Prussian blue which has a excellent electrocatalytic property to hydrogen peroxide reduction.The experimental conditions related to the preperation and characterization of sensor have been studied in detail.The results show that the optimun detection potential is 0.05V (vs.SCE) and the optimun pH is6.0.Under selective conditions the sensor showed excellent sensitivity of 80nA/mmol·L -1 and a linear range of 0.1~0.5mmol·L -1 .The apparent Michanelis\|Menten constant of the sensor is 21mmol·L -1 .The response time is less than 1.5min.In addition to good reproducibility of the enzyme biosensor,no apparent change in the response to glucose was observed after one month.Foremost,the interference of ascorbic acid and uric acid can be avoid with the low detected potential,thus the sensor is expected to detect the glucose in serum directly by the prensent method.
【Fund】: 上海市教委科学技术发展基金资助项目
【CateGory Index】: TP212
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