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《Chemical Research and Application》 2013-07
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Preparation and molecular recognition properties of indole-3-acetic acid-imprinted polymers

ZHANG Tie-li*,LIU Zheng-yuan,ZHANG Hui,MENG Qing-zhao (Tangshan Key Laboratory of Biology&Chemistry,Department of Chemistry,Tangshan Teachers College,Tangshan 063000,China)  
In order to synthesize molecular recognition material with high affinity and specificity for indole-3-acetic acid(IAA)and demonstrate the feasibility of such molecular spectrometric methods as UV and fluorometry for the selection of efficient functional monomer,4-vinylpyridine(4-Vpy)-ethyleneglycol dimethacrylate(EGDMA)-based polymers P(4-Vpy)and P`(4-Vpy)were prepared by bulk polymerization technique using IAA as the template molecule and two different amounts of acetonitrile as the porogen,respectively.The molecular recognition performances were evaluated by high-performance liquid chromatography.The results exhibited that both of the imprinted polymers showed the ability to recognize IAA and the amount of porogen used in the imprinting process exerted great influence on the imprinting effect.In addition,the comparison of molecular recognition ability was made between P(4-Vpy)and(P(AA))which was prepared under the same condition as P(4-Vpy)except using acrylamide(AA)as the functional monomer.The results indicated that P(4-Vpy)showed higher molecular imprinting efficiency for IAA than P(AA).The influence of different mobile phase on the retention of IAA and its structurally related compounds proved that electrostatic interaction played an important role in the molecular recognition process of P(4-Vpy).Furthermore,we made a comparative study on the interaction of IAA with 4-Vpy and AA by such spectroscopic techniques as UV and fluorometry.The results demonstrated that the complex of IAA-4-Vpy was more stable than that of IAA-AA,which can give a good explanation for the molecular recognition performances of P(4-Vpy)and P(AA).The study revealed that both UV and fluorometry can be utilized as effective means for the selection of efficient functional monomer.And the amount of porogen used in the preparation of MIP should be a major factor to be optimized to obtain MIP with high affinity and selectivity.This paper provides a foundation for the separation and enrichment of bioactive IAA.
【Fund】: 唐山市重点实验室(04360701B-6)资助项目;; 唐山师范学院博士基金(04D02)资助项目
【CateGory Index】: O631.3
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