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《Chemical Research and Application》 2014-07
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HPLC with switching wavelength simultaneous determination of six constituents in Gentiana crassicaulis

SONG Jiu-hua;YANG Wen-yu;MENG Jie;LI Yao;ZENG Yu;CHENG Tao;CHEN Xing-fu;College of Agronomy,Sichuan Agriculture University,Key Laboratory of Crop Physiology,Ecology and Cultivation in Southwest,Ministry of Agriculture;Department of Cchemistry,Leshan Normal College;Sichuan Huichuntang Pharmaceutical Company;  
To establish an HPLC with switching wavelength method for determination of six components(loganic acid,swertiamarin,gentiopicroside,sweroside,isoorientin,isovitexin) in Gentiana crassicaulis.Sepax Gp-C18(150 mm × 4.6 mm,5 μm) column was used,the mobile phase was methanol-0.1% phosphoric acid solution with gradient elution at a flow rate of 1.0 mL·min-1.The column temperature was set at 25℃,detection wavelength was 235 nm(0 ~ 51 min) 、270 nm(51 ~ 57 min) 、243 nm(57 ~ 65 min) 、270 nm(65 ~ 80 min).The six components(loganic acid,swertiamarin,gentiopicroside,sweroside,isoorientin,isovitexin) content in the ranges of 0.01000 ~ 10.0000,0.00216 ~ 2.1600,0.048 ~ 48.0000,0.0010 ~ 1.0000,0.00034 ~ 0.3400 and 0.00026 ~0.2600 μg respectively,had a good linearity.The average recoveries were 98.52% ~ 99.31%,the RSD was less than 3.5%.There were significant correlations between the content of the four iridoid glucosides,the content of the two flavone constituents had correlations too.There was no correlations between the flavone constituents and the iridoid glucosides constituents.Conclusion:This method was simple,rapid,accurate,dependable.And may be use to control quality of Gentiana crassicaulis.
【CateGory Index】: O657.72;R284.1
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