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《Journal of Sichuan University (Medical Sciences Edition)》 1990-03
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Human Sex Identification in Dried Bloodstains by Analysis of Amplified DNA Sequences

Sun Guangyun;Wu Meiyun Department of Forensic Serology  
This paper describes a technique ofDNA amplification in vitro and its applica-tion on sex identification of human driedbloodstains. Dried bloodstains (8 males and8 females) were prepared on filter paper.and then DNA was extracted. Target DNAsequences (Y-3.4kb repeat and Alu-repeat)were amplified by polymerase chain reac-tion (PCR) with two pairs of primers(Y1.1, Y1.2 and Alu9.1, Alu9.2). Amplifi-cation products were obtained in 8 male'sbloodstains with the primers Y1.1 andY1.2, but at in 8 female's bloodstains; andin 4 male's and 4 female's bloodstainswith Alu9.1 and Alu9.2.
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