Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《华西医科大学学报》 1991-01
Add to Favorite Get Latest Update

A Study of DEAE Ion Exchange Chromatography for the Purification of Hen's Yolk IgG

Yang De;Wong Daoruo Department of Micribiology and Immunology  
A DEAE ion exchangechromatography procedure performed byusing either gradient or stepwise elutionwas dereloped for the purification ofchicken IgG antibodies from the egg yolkof immunized hens. Following this proce-dure,highly purified yolk IgG preparationswere obtained as identified by SephadexG 200 gel filtration as well as sodium do-decyl sulfate-polyacryamide gel electro-phoresis. Moreover,this procedure guaran-teed the recovery of antibodies of undi-minished activity.The results demonstratethat the procedure is simple,reliable. andpractical for the yolk IgG purification.Theestablishment of such a method is benefi-cial to the production and application ofegg yolk antibodies.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 6 Hits
1 Yin Zhixin, Huang Shuqi (Guangzhou Medical College, PLA. Guangzhou,510315);Purification of chicken egg yolk IgG by precipitation of sodiuin sulfate and ion exchange chromatography[J];;1995-02
2 LIU Fei1,2,TANG Wei-jie1,HAN Qing-lin1,3,ZHAO Ting-ting1,GAO Li-qin1,YAN Xiao-ling1(1.College of Veterinary Medicine,Sichuan Agricultural University,Ya'an 625014,China;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province,Ya'an 625014,China;3.Bureau of Animal Husbandry of Daying,Sichuan Province,Daying 629300,China);Preparation of Rabbit Anti-goose IgG-HRP and Preliminary Application[J];China Animal Husbandry & Veterinary Medicine;2011-06
3 SONG Hong-xin,LIU Xuan,LIANG Yan (College of Life Science and Engineering, Shaanxi University of Science and Technology, Xianyang 712081, China);Study on Seperation and Purification of Immunoglobulin in Yolk[J];Food Science;2005-04
4 Yin Zhixin,etal .(Guangzhou Medical College of PLA, 510315);Preparation and Preliminary Characterization of Monoclonal Antibodies to Chicken Egg Yolk IgG[J];JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY;1996-01
5 XUE Hai-yan,SONG Hong-xin (College of Life Science and Engineering, Shaanxi University of Science & Technology, Xianyang 712081, China);A REVIEW OF THE PURIFICATION METHOD ON IMMUNOGLOBULIN Y[J];Journal of Shaanxi University of Science & Technology;2003-04
6 LIU Fei1,2,TANG Wei-jie1,CHENG An-chun1,2,WANG Ming-shu1,2,ZHAO Ting-ting1 HAN Qing-lin1,3,GAO Li-qin1(1.College of Veterinary Medicine,Sichuan Agricultural University,Ya'an 625014,China;2.Key Laboratory of Animal Diseases and Human Health of Sichuan Province,Ya'an 625014,China;3.Bureau of Animal Husbandry of Daying,Sichuan Province,Daying 629300,China);Preparation of anti-GPV yolk IgG and development of an indirect-ELISA for detection of goose parvovirus infection in goose[J];Chinese Veterinary Science;2011-06
Chinese Journal Full-text Database 1 Hits
1 Yang De;Wang Daoruo Department of Microbiology and Immunology;Comparative Study of Two Methods for the Extraction of Yolk IgG[J];;1990-03
Chinese Journal Full-text Database 1 Hits
1 DENG Wei yi,FANG Min hua,GAO Mei mei,YAN Qi jiang (Institute of Neuroscience,Second Affiliated Hospital, Guangzhou Medical College,Guangzhou,Guangdong 510260);Neutralization of Hen Yolk Antibodies Against Agkistrodon Halys Venom[J];Academic Journal of Guangzhou Medical College;2002-02
Chinese Journal Full-text Database 10 Hits
1 MIN Si- pan et al (Department of Food Science, JAU, Nanchang, Jiangxi 330045);Propolis as in Food Anti-staling Agent[J];Journal of Anhui Agricultural Sciences;2006-08
2 ZHANG Hong - man , OUYANG Ping - kai, YU Wen - tao, LU Qian ( Department of Applied Chemistry, Nanjing University of Technology, Nanjing 210009 , China; College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009, China);Determination of Amino Acids in Astragalus complanatus by Gas Chromatography / Mass Spectrometry[J];Amino Acids & Biotic Resources;2004-02
3 LI Jun~(1,4),FU Fang~3,YUAN Xiao-yuan~2,CUI Shang-jin~3,TONG Guang-zhi~3,WEN Jian-xin~4,WANG Jin-bao~(1)(1.Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan 250100,China;2.Institute of Poultry,Shandong Academy of Agricultural Sciences,Jinan 250100,China;3.Harbin Veterinary Research Institute,CAAS,Harbin 150001,China;4.LaiYang Agricutrual College,Qingdao 266109,China);Expression of Eukaryotic Expression Vector Including ORF5/ORF6 of Porcine Reproductive and Respiratory Syndrome Virus Strain SD2 in Mammalian Cells[J];Chinese Journal of Virology;2006-06
4 FENG SHU-LIN, BEN KUN-LONG, WANG YUN-MEI (Kunming Institute of Zoology, Academia Sinica, Kunming, Yunnan 650223) LIANG ZHI-Guo (National Research Institute for Family Planning, Beijing 100081, China);LOCALIZATION OF LACTATE DEHYDROGENASE C4 (LDH-C4) ON SPERMS BY IMMUNOFLUORESCENCE ASSAY WITH MONOCLONAL ANTIBODIES[J];Acta Zoologica Sinica;1995-01
5 ZHANG Xiao Ping,HUANG Hao Long,ZHU Zu Kang,CHEN Jie,ZHAO Ru Qian ** Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China;Absorption of cholecystokinin (CCK) yolk antibody from the duodenum of the rat[J];Acta Zoologica Sinica;2004-03
6 ZHANG Ai-ping1,LIU Xiang1,LI Yong-cai2,HU Ai-ping3(1.Zhejiang University,Hangzhou 310029,China;2.Qiandaohu Yongcheng Green Food Co.Ltd.,Hangzhou 311512,China 3.Technology Division of Chun′an,Hangzhou 311700,China);Preservation of Aquatic Products During Storage and Transportation[J];Packaging and Food Machinery;2009-05
7 GUO Ya-cui1,3,DU Qin-sheng2,ZHU Ming-li3,ZHANG Yu-fan3(1.School of Biological Science and Technology,Changchun University,Changchun 130022,China;2.School of Computer Science and Technology,Changchun University,Changchun 130022,China;3.Training Center of Jilin Provincial Quality and Technical Supervision,Changchun 130022,China);The application of freeze-dried technology in food industry[J];Journal of Changchun University;2008-02
8 FANG Min,SHEN Yuexin(Food College of Shanghai Fisheries University,Shanghai 200090,China);Ozone and Its Application in the Freshness Keeping of Aquatic products[J];Fisheries Science;2003-04
9 YAN Wei~1,WU Yan-yan~(1,2),LI Lai-hao ~2 (1.College of Food,Ocean University of China,Qingdao 266003,China;2.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China);Application of Enzyme Preparation in Preservation of Fishery Product[J];Fisheries Science;2006-12
10 WANG Hui-e(Key Laboratory of Animal husbandry Science and Technology, Xinjiang Production and Construction Corps,Alaer 843300,China);Advances on the Technology of Sperm Separation[J];Grass-Feeding Livestock;2008-01
China Proceedings of conference Full-text Database 1 Hits
1 CHENG Zhi-ping CHENG An-chun WANG Ming-shu CHEN Bing LIU Chuang DUAN Kun ZHOU Xue GONG Yong-qiang CHEN Xiao-yue (1.Poultry Disease Research Center of Sichuan Agricultural University,Yaan 625014,China;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province,Yaan 625014,China;3.Sichuan Provincial Animal Epidemic Prevention &,Supervision General Office,Chengdu 610041,China;3.Research Center of Animal Microecological Engineering,Sichuan Agricultural University,Yaan,Sichuan,625014,P.R.China);Preliminary Study on Effect of Cell Mediated Immunity Regulation of Duck Enhanced by Duck IFN-α Eukaryon Expression Plasmid Inoculated with Gene-gun when DPV Attenuated Vaccine Fellow Vaccination[A];[C];2008
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YIN Rong-lan,ZHANG Nai-sheng,YANG Zheng-tao,FENG Xiao-sheng (College of Animal Science and Veterinary Medicine,Jilin University,Changchun,Jilin,130062,China);Progress on Prevention and Treatment of Cow Mastitis[J];Progress in Veterinary Medicine;2007-05
2 ZHU Ping~1,FU Ai-fen~2,WU Ping-ping~2,CHEN Xing-yu~1,ZHANG Lan~1,YANG Jie~1,LI Hui~2,TIAN Ye~2,YI Jing~1(1 Department of cell biology,2 pathology,Shanghai second medical university,Shanghai 200025,China);Morphological study on anti-Streptococcus mutans IgY against dental caries[J];Journal of Chinese Electron Microscopy Society;2004-06
3 Yin Zhixin,Yang Yi,He Xiaojuan et al(Department of Biochemistry, Guangzhou Medical College, PLA. Guangzhou, 510315);Preparation of chicken anti-HBs and its application in the detection of human HBsAg[J];;1996-02
4 HUANG Qing-song1,2,LUO Shen-qiu1,LIN Yuan-zao3,ZENG Wei-sen1(1.School of Basic Medical Sciences,Southern Medical University,Guangzhou 510515;2.Department of Medical Biology,Guangdong Pharmaceutical University,Guangzhou 510006;3.Department of Biochemistry and Molecular Biology,Guangdong Pharmaceutical University,Guangzhou,Guangdong 510006,China);The preparation and character investigation of the immunoglobulin of yolk(IgY) produced by methicillin-resistant staphylococcus aureus(MRSA)[J];Journal of Guangdong College of Pharmacy;2008-02
5 REN Ping-guo, XU Qi-hong (Luohe Vocational Technology College, Luohe 462000, China);Analysis on the Separation and Purification of Immunoglobulin of egg yolk(IgY)[J];Modern Food Science and Technology;2007-11
6 JIANG Qian-zhou,MIN Zhi-peng,YI An-hua,et al.Stomatology hospital of Guangzhou Medical University,Guangzhou 510140;The Effect of the Anti-dental Carious IgY and its Dentifrice on the Adherence of Streptococcus Mutans[J];Journal of Oral Science Research;2012-08
7 RAO Gui-bo;SHAO Hong-ze;HU Gui-xue;WU Jian-min;Guangxi Veterinary Research Institute;Jilin Institute of Veterinary Science;Jilin Agricultural University;;Isolation,Identification and Pathogenicity of a Strain of GPV[J];Acta Ecologae Animalis Domastici;2014-01
8 RAO Gui-bo;SHAO Hong-ze;HU Gui-xue;WU Jian-min;Guangxi Veterinary Research Institute;Jilin Veterinary Science Institute;Jilin Agricultural University;;Development and Preliminary Application of PCR-DHPLC for Goose Parvovirus Detection[J];Acta Ecologae Animalis Domastici;2014-05
9 PI Jin-kui,ZHANG Huan-rong(Life Science and Technology College of Southwest University for Nationalities,Sichuan Chengdu 610041,China);Comparison of two methods for extracting IgY[J];Sichuan Animal & Veterinary Sciences;2011-10
10 CHI Zheng-bing,GAO Yan-xia,PANYing, ZHANG Bin, FENG Xi-pin.(Department of Preventive & Pediatric Dentistry, Ninth People's Hospital, School of Stomatology, Shanghai Second Medical University. Shanghai 200011; Shanghai Research Institute of Stomatology. Shanghai 200011,China);The inhibitive effect of IgY toothpaste against oral Streptococcus mutans[J];Shanghai Journal of Stomatology;2004-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved