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《华西医科大学学报》 1991-01
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A Study of DEAE Ion Exchange Chromatography for the Purification of Hen's Yolk IgG

Yang De;Wong Daoruo Department of Micribiology and Immunology  
A DEAE ion exchangechromatography procedure performed byusing either gradient or stepwise elutionwas dereloped for the purification ofchicken IgG antibodies from the egg yolkof immunized hens. Following this proce-dure,highly purified yolk IgG preparationswere obtained as identified by SephadexG 200 gel filtration as well as sodium do-decyl sulfate-polyacryamide gel electro-phoresis. Moreover,this procedure guaran-teed the recovery of antibodies of undi-minished activity.The results demonstratethat the procedure is simple,reliable. andpractical for the yolk IgG purification.Theestablishment of such a method is benefi-cial to the production and application ofegg yolk antibodies.
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1 Yang De;Wang Daoruo Department of Microbiology and Immunology;Comparative Study of Two Methods for the Extraction of Yolk IgG[J];;1990-03
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