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《Journal of West China University of Medical Sciences》 2000-03
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The Modulation of Type Ⅰ Collagen and Its Receptor Syst em on Biological Characteristics of Osteoblasts

Yang Zhiming, Yu Xijie, Qu Yi, Xie Huiqi (Department of Orthopedic Surgery, The First Affiliated Hospit al, WCUMS, Chengdu610041)  
This paper addresses the question whether type Ⅰ col lagen receptor system is necessary for the functional activity of osteoblasts. Through blocking type Ⅰ collagen receptor system by type Ⅰ collagen antibody or integrin α2β1 antibody, the proliferation of osteoblasts was studied by cell count, the apoptosis was studied by flow cytometry, and the mRNA expressio ns of type Ⅰ collagen, integrin α2β1 and osteocalcin were also detected b y RT PCR techniques. When type Ⅰ collagen receptor system was blocked, osteobl asts showed high apoptosis rate, lower growth kinetics and weak mRNA expression of type Ⅰ collagen, integrin α2β1 and osteocalcin; the blocking effect wa s reversible. It suggests that type Ⅰ collagen receptor system is necessary for osteoblastic phenotype. The biomaterials for bone tissue engineering should be constructed according to the extracellular matrix of osteoblasts, which supply n ormal extracellular environment for osteoblasts.
【Fund】: 国家重点基础研究发展规划(973)项目;; 组织工程的基本科学问题(批准号G1999054308);; 国家自然科学基金! (批准号39830100) 资助
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