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《Journal of West China University of Medical Sciences》 2001-01
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The Influence of Type Ⅰ Collagen on the Cell Behavior o f Human Embryoni c Periosteous Osteoblasts

Yang zhiming,Yu xijie, Huang fuguo, Xie Huiq i. Department of Orthopedic Surgery, The First Affiliated Hospital, WC UMS, Chengdu 610041, China  
Objective To study the influence of type Ⅰ coll agen(COL Ⅰ) on the cell behavior of human periosteous osteoblasts(OB) and the application of type Ⅰ collagen in constructing bioactive artifical bone.Methods OB were cultured on dishes coated with bovine type Ⅰ collage n in different final concentrations. The cell adhesion was examined by the meth o ds of cell count, the proliferation of OB was studied by 3H TdR, and the ost eoblastic ability was assessed by the synthesis of collagen, osteocalcin and alk aline phosphatase (ALP). Results OB cultured on type Ⅰ collage n layer had the following characteristicis: ① The amounts of adhesive cells we re maximal top in 25 μg/ml final concentration; ② The proliferation of OB was decreased above 12.5 μg/ml final concentration ( P 0.05); ③ The synthesis of type Ⅰ collagen was reduced slightly (above 25 μg/ml, P 0.05); ④ The secretion of osteocalcin was increased markedly (above 6.25 μg/ml, P 0.05, w hich reached maximally in 25μg/ml); ⑤ The ALP activity was also increased (ab ove 12.5μg/ml, P 0.05). Conclusion Type Ⅰ collagen promot es the expression of osteoblastic phenotype and cell adhesion. When the scaffold materials for bone tissue engineering are coated with type Ⅰ collagen, the ost eogenesis of OB is enhanced to accelerate the tranformation course from artifici al bone to biological bone, the best final concentration is 25μg/ml.
【Fund】: 国家重点基础研究发展规划 (973) -组织工程的基本科学问题资助项目! (批准号 G19990 432 0 8);; 国家自然科学基金重点资助项目 !(批准号
【CateGory Index】: R329.28
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