Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of West China University of Medical Sciences》 2001-03
Add to Favorite Get Latest Update

p53 Mutation in Lung Cancer in Gejiu and Xuanwei Regions of Yunnan Province

Huang Yanping, Jin Kewei, Gao Qian, Li Baogang. Department of Pathology, Kunming Medical College, Kunming 650031, China  
Objective The incidence rates of lung cancer among tin miners in Gejiu and among peasants in Xuanwei are higher than the average incidence rate in Yunnan Province, and the causes of the disease in these two regions have been preliminarily clarified. This study was directed to the molecular biological basis of its pathogenesis.Methods Twenty six specimens of the lung cancers of Gejiu tin miners(GTM) and 15 ones of Xuanwei peasants (XP) were examined by single strand conformation polymorphism analysis of polymerase chain reaction products.Results Using PCR primers for the most highly conserved region of the p53 including exons 5 8, we have identified p53 mutations in 13 lung cnacer tumor DNA samples of GTM and in 4 ones of XP. Nucleotide sequencing of one of the exon 5 mutations of GTM indicated that there was a C to T transition, which resulted in substitution of Val for Ala at amino acid 135, and there was an AAC to TTC transertion in the exon 7 of XP, which resulted in substitution of Phe for Asn at amino acid 247.Conclusion p53 mutation is a frequent incident in lung cancer of GTM and XP, and two new mutation sites were found in p53 gene, which have not been reported before now. There was no statistically significant correlation between the detection of p53 mutation and the patient's age, sex, mining history and histologic type of the cancer.
【Fund】: 国家自然科学基金 (批准号 3 93 6 0 0 2 8);; 云南省应用基金资助 (96 0 0 71M)
【CateGory Index】: R734.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 2 Hits
1 CHAI Qin,WANG Wei-ming.;The Effect of the Phosphatidylinositol 3′-Kinase-AKT Inhibitor on Proliferation of Lung Cancer cell[J];Journal of Clinical Pulmonary Medicine;2011-02
2 ZHOU Li~1 JIN Ke-wei~(1*) ZHANG Tian-bao~2 (1.Department of Pathology in Kunming Medical College,Kunming 650031,China;2.Department of Toxicology in Second Military Medical University,Shanghai 200433,China);Expression of P53,MDM2,P21,P16 in Malignant Transformation Cells of Human Bronchial Epithelial Cells Induced by Yunnan Tin Mine Dust[J];Journal of Environmental & Occupational Medicine;2007-03
Chinese Journal Full-text Database 1 Hits
1 Yunnan tin mirer' lung cancer research group. depart-ment of Parhology, Kunming Medical College, Kun-ming 650031;Etiological studies on Yunnan tin miners’lung cancer .[J];CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES;1995-01
Chinese Journal Full-text Database 10 Hits
1 YUAN Su bo\+1,WANG Zhi qiao\+1,YE Chang qing\+2,LIAO Ming yang\+1 (1.Institute of Pharmacology and Toxicology, Beijing 100850, China; 2.Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China);CYTOGENETIC ANALYSIS OF TRANSFORMED HUMAN BROCHIAL EPITHELIAL CELLS INDUCED BY CYCLOPHOSPHAMIDE AND THIOTEPA[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;2000-04
2 MENG Zi qiang\+1, ZHANG Lian zhen\+2 (1. Institute of Environmental Medicine and Toxicology, Shanxi University, Taiyuan 030006,China; 2. China Institute for Radiation Protection, Taiyuan 030006, China);DNA OXIDANT DAMAGE INDUCED BY TOBACCO SMOKE IN HUMAN BLOOD LYMPHOCYTES[J];Carcinogenesis,Teratogenesis and Mutagenesis;2001-03
3 FAN Bao-xing,ZHANG Kai-tai, XIE Ling, XIANG Xiao-qiong, WU De-chang(Department of Molecular Toxicology, Beijing Institute of Radiation Medicine, Beijing 100850, China);CONSTRUCTION OF DIFFERENTIALLY EXPRESSED cDNA LIBRARIES FROM DISSENMILATORY MALIGNANT TRANSFORMED HUMAN BRONCHIAL EPITHELIAL CELLS INDUCED BY ALPHA-PARTICLE RADIATION[J];Carcinogenesis,Teratogenesis and Mutagenesis;2002-01
4 WANG Wei 1,2 ,WU Yong-jun 1 ,WU Yi-ming 1,ˇ (1.Department of Occupational Health and Health Toxicology,College of Public Health,Zhengzhou University,Henan Zhengzhou450052, China;2.Zhengzhou Institute of Occupational Disease, Zhengzhou Henan450053,China);Genetic Polymorphism in hOGG1 and Susceptibility to Lung Cancer[J];Carcinogenesis,Teratogenesis and Mutagenesis;2005-02
5 ZHOU Li1,JIN Ke-wei1,ZHANG Tian-bao2(1. Department of Pathology Kunming Medical College,Yunnan Kunming 650031;2. Department of Toxicology in Second Military Medical University,Shanghai 200433);Malignant Transformation of Immortalized Human Bronchial Epithelial Cells BEAS-2B Induced by Tin Mine Dust in Yunnan Province[J];Carcinogenesis,Teratogenesis & Mutagenesis;2006-05
6 ZHANG Hui-zhong LU Xing-yu ZHAO Mei-qin(Pathological Department of Tumor Hospital, Sun Yat-sen University of MedicalSciences, Guangzhou. 510060);1MMUNOHISTOCHEMICAL STUDY ON MONONUCLEAR CELLS AND BETA-2 MICROGLOBULIN IN TISSUE OF LUNG CARCINOMA[J];Chinese Journal of Cancer;1993-04
7 Jin Kewei;Gao Qian;Sun Laihua;et al(1 Department of pathology, Kunming Medical College, Kunming 650031 2 Larbour-Health Institute of Yunnan Tin Industry company, Gejiu 661400);A Study of the p53 Tumor Suppressor Gene in Yunnan Tin Miner's Lung Carcinoma[J];Chinese Journal of Cancer;1999-01
8 GUI Shuyu 1、2 , WANG Yuan1, LIU Hu1, et al 1 Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, PRChina 2 Department of Respiratory Medicine, the First Affiliated Hospital of Anhui Medical Uni;The clinical significance of deletion of p16 gene homozygous deletion in malignant pleural effusion[J];CHINESE JOURNAL OF CANCER;2000-03
9 CHEN Jie* , QIAN Gui- sheng, HUANG Gui- jun, XIONG Wei, LI Jing Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R.China;Suppression Subtractive Hybridization Identified Genes Differentially Expressed in a Multidrug Resistance Cell Line of Human Lung Adenocarcinoma[J];Chinese Journal of Cancer;2001-04
10 ZHU Mao-xiang * ,YANG Zhi-hua,GONG Yi-fen,LU Ying,CAO Zhen-shan Institute of Radiatio n Medicine,Academy of Military Medical Science,B eijing 100850,P.R.Ch ina;Initiated Cancerazation Effect of Reactive Oxygen Species and Its Produ cts of Oxidative DNA Damage in B EP2D Cells[J];Chinese Journal of Cancer;2001-10
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved