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《Journal of West China University of Medical Sciences》 2001-04
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Study of Quinolones Resistance in Escherichia coli, Staphylococcus aureus and Pseudomonas areuginosa

Feng Ping, Yu Rujia, Xia Peiyuan, Lü Xiaoju, Zhu Shuyuan. Department of Infectious Diseases, The First Affiliated Hospital, WCUMS, Chengdu 610041, China  
Objvective To investigate the factors of quinolone resistance mediating in Escherichia coli(E.coli), Staphylococcus aureus (S.aureus) and Pseudomonas areuginosa(P.areuginosa) and examine the characteristic changes and quinolones resistance of these bacteria after exposing them to quinolones,dye materials, and ultra violet respectively. Methods The agar plate inoculating on surface and tube broth two fold dilution methods were adopted. Results Quinolone resistance was mainly mediated by repeated exposure of the bacteria to low level concentration of quinolone. No quinolone resistance was found after exposure of the bacteria to ultra violet and dye materials such as Ethidium bromide and Acry orange. Among the three kinds of bacteria, E. coli tended to have stable high level of quinolone resistance(MICs≥256mg/L). S.aureus acquired mediate level of quinolone resistance(MICs≤32mg/L). P.aeruginosa acquired a high but not stable level(MICs≥256mg/L)of resistance. These bacteria were cultured in the concentration of 1/2×MIC, 1×MIC, 2×MIC, 4×MIC, 5×MIC ciprofloxacin, and were compared with those cultured in the same concentration of sparfloxacin and cefotaxime. After being affected by ciprofloxacin, the E.coli underwent apparent morphologic changes, such as becoming wider(2 3 times),longer(10 30 times),thread like, and fewer in number. These changes were consistent with the increase of quinolone concentrations. The morphologic changes in P.aeruginosa and S.aureus were not so obvious as those in E.coli. Sparfloxacin and cefotaxime mainly led to the decrease in the numbers of bacteria. When the concentration of Sparfloxacin and cefotaxime were 4×MICs, the changes of P.aeruginosa and S.aureus were less obvious. Conclusion The bacteria's quinolone resistance is mainly induced by the quinolone itself. The quinolone resistance is more likely occur in E.coli than in P.aeruginosa and S.aureus. After exposure of the bacteria to ciprofloxacin, E.coli might have most apparent morphologic changes and occurrence resistance. [
【Fund】: 国家自然科学基金资助 (批准号 395 0 0 12 8)
【CateGory Index】: R446.5
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