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《Journal of West China University of Medical Sciences》 2002-01
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Effect of Acupuncture on the Expression of NT_3 in the Process of Spinal Plasticity

Wang Tinghua *, Wu Liangfang, Liao Deyang, Zhou Xue, Chen Yanhong, A.Takeda. *Department of Histology and Embrology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China  
Objective To explore the change in the expression of NT 3 in the process of promoting the plasticity of spinal cord by acupuncture. Methods Five adult cats were subjected to unilateral spared root rhizotomy; their L 1 L 5, L 7 S 2 dorsal root ganglia (DRG) were sectioned, but L 6 was spared. And two groups of acupoints 〔Zusani (St.36) and Xuanzhong (G.B.39); Futu (St.32) and Sanyingjiao (Sp.6)〕 located in hind limb were electro stimulated for thirty minutes q.d.×7. At seven days, after acupuncture, the L 5 segment of spinal cord and spared dorsal root ganglion (L 6) were taken and made into frozen section 20 μm in thickness. Immunohistochemistry (NT 3 antibody 1∶1500) and in situ hybridization (NT 3 cRNA probe 1∶100) techniques were used. The numbers of positive neuron for NT 3 and it's mRNA in large, medium, small neuron of L 6 DRG and the numbers of positive neurons and glia cells for NT 3 in lamina Ⅱ were counted respectively. Results The numbers of positive large,small neurons for NT 3 and its mRNA in DRG and the number of positive neurons and glia cells for NT 3 in lamina Ⅱ on the acupuncture side increased apparently than those on the non acupuncture side ( P 0.05). However, the positive signal of NT 3 mRNA in lamina Ⅱ was not seen in our study. Conclusion The results indicate that acupuncture promoting the plasticity of spinal cord involves both the increase in expression of NT 3 in large and small neurons of spared DRG and the increase in number of NT 3 positive neurons and glia cells in spinal lamina Ⅱ. Moreover, NT 3 may play a role in the process of promoting the plasticity of spinal cord by acupuncture.
【Fund】: 国家自然科学基金资助 (批准号 3980 0 191)
【CateGory Index】: R245
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