Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Sichuan University(Medical Science Edition)》 2012-03
Add to Favorite Get Latest Update

Effect of Sinusoidal Electricity Magnetic Fields on the Proliferation and Differentiation of Osteoblasts in vitro

WANG Jia-qi1,GE Bao-feng1,ZHOU Jian1,WEI Zhe2,HAN Gui-qiu1,ZHU Rui-qing1,CHEN Ke-ming1.1.Institute of Orthopaedics,Lanzhou General Hospital of PLA,Lanzhou 730050,China;2.Institute of Medical Engineering,Lanzhou General Hospital of PLA,Lanzhou 730050,China  
Objective To investigate the effect of sinusoidal electricity magnetic fields(SEMFs) on the proliferation and differentiation of osteoblasts in vitro.Methods Calvarial osteoblasts of newborn rats were isolated by enzyme digestion and randomly divided into 3 groups after subculture.Two groups of cells were exposed to 50 Hz 1.8 mT SEMFs for 30 min/d in parallel and vertical,respectively.Those without SEMFs exposure served as control.The cells were observed under the contrast phase microscope each day.After 48 h,cell proliferation was assayed by MTT method.The alkaline phasphatase(ALP) activities and calcium contents were measured after 3,6,9,and 12 days.The ALP positive colonies were histochemically stained after 10 days and the calcified nodules were stained by Alizarin Bordeaux after 12 days.Expressions of ALP,bone morphogenetic protein-2(BMP-2) and Osterix(OSX) mRNA were measured at 0 h,24 h,48 h and 96 h.Results The cells exposed to the SEMFs were arranged in spiral appearance after 3 days.Compared with control,SEMFs inhibited cell proliferation(P0.01 or P0.05),but enhanced the maturation and mineralization of the osteoblasts.The results showed that SEMFs improved ALP activities,promoted calcium contents,increased calcified nodulues numbers,boosted expressions of ALP,BMP-2 and OSX mRNA.SEMFs with magnetic lines of force in parallel has stronger activities than those in vertical.Conclusion The SEMFs at 1.8 mT and 50 Hz inhibit the proliferation of osteoblasts,but enhance the maturation and mineralization of osteoblasts.
【Fund】: 甘肃省科技重大专项项目(09ZNKDA025)资助
【CateGory Index】: R329
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Yu Lei,Luo Erping,Shen Guanghao,Han Lihua,Wu Xiaoming;Effect of pulsing electromagnetic fields on treatment of osteoporosis[J];Modern Rehabilitation;2003-12
2 ZOU Lin-yue,WANG Zhen-bin,Ahmat (Department of Central Laboratory,First Affiliated Hospital,Xinjiang Medical University,Urumqi 830054,China);Biologic features of osteoblast and its culture in vitro[J];Journal of Xinjiang Medical University;2004-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 FANG Dong-mei1,ZHOU Dao-hua2,ZHOU Feng-lin3 (1.Xuzhou Normal University,Xuzhou 221116,Jiangsu,China;2.Doctor Graduate,Suzhou University,Suzhou 215021,Jiangsu,China;3.Linhuan Coal Mine Hespital,Huaibei Coal Group,Suixi 235136,Anhui China);Effects of Different Impact Training on Mineralization and Mechanical Properties of Growing Rats' Bone[J];Journal of Beijing Sport University;2007-04
2 ZHAO An-ju1,HUANG Song-min2,OU San-tao2,et al.(1.Department of Nephrology,Huaxi Hospital,Sichuan University,Chengdu 640000,China;2.Department of Cardres,Guizhou Provincial People′s Hospital,Guiyang 550001,China);Significance of BMP-2 expression at renal arteriole in diabetic nephropathy rats[J];Chongqing Medicine;2010-22
3 MA Zong-min~(1),LI Shu-xian~(2),ZHU Xinghua~(3) (1.Institute of Biomedical Engineering,Dalian University,Dalian 116021,China;2.Academician Innovation Park,Dalian University,Dalian 116622,China;3.Department of Mechanics,Jilin University,Changchun 130025,China);Numerical simulation of process of osteoporosis in women[J];Journal of Dalian University;2006-04
4 XU Qiao-Ling 1,LU Li-Hua 2,LUO Er-Ping 3, SHEN Guang-Hao 3, LIU Dong-Huan 1, HUA Qian-Zhen 1, WU Xiao-Ming 3, LI Li-Rong 3 1School of Nursing, 2Department of Chemistry, School of Basic Medicine, 3Department of Military Medical Equipment & Metrology, School of Biomedical Engineering, Fourth Military Medical University , Xi’an 710033, China;Effects of low intensity PEMFs on bone mineral density and histomorphometry in SD rats[J];Journal of The Fourth Military Medical University;2004-21
5 LUO Er-Ping1,JIAO Li-Cheng1,SHEN Guang-Hao2,WU Xiao-Ming2,XIE Kang-Ning2, LU Li-Hua3,XU Qiao-Ling4 1Research Center of Intelligent Information Processing, School of Electronic Engineering, Xidian University, Xi'an 710071, China, 2Department of Military Medical Equipment & Metrology, School of Biomedical Engineering, 3Department of Chemistry, School of Pharmacy, 4School of Nursing, Fourth Military Medical University , Xi'an 710033, China;Development of GZY therapeutic instrument for osteoporosis[J];Journal of the Fourth Military Medical University;2006-03
6 HUANG Su-Ran1, LUO Er-Ping2, SHEN Guang-Hao2, LI Pu-Qian1, WANG Jing-Wen1, ZHOU Qian-Jun1, XIE Kang-Ning21Department of Obstetrics and Gynecology, Dongguan People's Hospital, Dongguan 523000, China, 2Department of Military Medical Equipment and Metrology, School of Biomedical Engineering, Fourth Military Medical University, Xi'an 710033, China;Effects of electromagnetic fields on osteoporosis in ovariectomized rats[J];Journal of the Fourth Military Medical University;2007-06
7 SHEN Hui, PAN Shi-zhongDepartment of Endocrinology, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China;Osteoporosis Model of the Ovariectomized Rat[J];Journal of Fujian Medical University;2004-02
8 ZHENG Jing, LU Hui-ling, WANG Ding-zhao, et al. ~△ Department of Forensic Biology, Preclinical school of Sun Yat-sen University, Guangzhou 510089, China);Histological study on species identification by plexiform bone and osteon banding[J];Chinese Journal of Forensic Medicine;2004-06
9 WANG Shi-zhong,LIN Han-ling,SHI Li-hua(The Second Affiliated Hospital,FCTCM,Fuzhou,Fujian 350003,China);Pulse Electromagnetic Stimulation Combined Digital Acupoint Pressure in Treating 48 Cases of Osteoporosis[J];Journal of Fujian College of Traditional Chinese Medicine;2006-04
10 Chen Jianlin1,Yang Zhe1,Tang Yanfeng2,3,Hu Xueqing1,Zhang Jizhong1,Zhou Ye1,Zheng Xiaohui2.(1.Dept.of Stomatology,Lingwu People's Hospital of Muslim Population Autonomous Region in Ningxia,Lingwu 750400,China;2.Dept.of Traumatic and Plastic Surgery,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China;3.Dept.of Stomatology,Baotou Central Hospital in Mongolia Autonomous Region,Baotou 014040,China);Study on collagen membrane as growth factors delivery carrier materials to accelerate mandibular fracture healing[J];International Journal of Stomatology;2011-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Zhi ying 1, WANG Xing 1△ , XIAO Jun jun 2, DONG Xiao min 2, MENG Shu cong 2, ZHAO Man 2 (1. Peking University School of Stomatology, Beijing 100081,China; 2. Department of Cell Biology & Genetics, Peking University);Culture of osteoblasts by serial explant culture and comparison of their characteristics[J];Journal of Peking University(Health Sciences);2004-01
2 LI Yong-hua,ZHU Shu-xia,SONG Zhi-yuan,et al.(Department of Cardiology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Study the effects of pulsed electromagnetic fields on the differentiation of rat marrow mesenchymal stem cells induced with 5-azacytidine[J];Chongqing Medicine;2008-04
3 YAN Ai min,LIU Li,CHEN Bing Li,et al. (Department of Orthopadies,Central Hospital of Handan City,Handan 056001,China);Primary culture and identification of the rabbit embryo osteoblasts[J];Journal of Traumatic Surgery;2002-S1
4 YANAi min ,LIULi,CHENBing li,etal. (DepartmentofOrthopadies,CentralHospitalofHandan ,HebeiProvince ,Handan 0 5 6 0 0 1,China);Experimental study of transplantation of rabbite mbryo osteoblasts for repair of bone defect of allogeneic rabbit[J];Journal of Traumatic Surgery;2003-02
5 LIU Hou-Li1, CHEN Jin-Wu2, WANG Lian-Cai1, WANG Chang-Yong2, FENG Zeng-Guo 1* (1. School of Material Science and Engineering, Beijing Institute of Technology, Beijing 100081, China; 2. Tissue Engineering Research and Development Center, Beijing Institute of Basic Medical Sciences, Academy Military Medical Science, Beijing 100850, China);Preparation of PEGT/PBT Tissue Engineering Scaffolds by Solution Spray Method[J];Chemical Research In Chinese Universities;2005-01
6 CHEN Fuping,HU Qiaoling,CHEN Liang,LI Baoqiang,SHEN Jiacong(Institute of Polymer Composites,Zhejiang University,Hangzhou 310027);PREPARATION OF MAGNETIC IRON OXIDE/HYDROXYAPATITE/CHITOSAN RODS BY in situ PRECIPITATION[J];Acta Polymerica Sinica;2006-06
7 YANG An\|le,SUN Kang,WU Ren\|jie (Key Lab on Metal Matrix Composites,Shanghai Jiaotong University,Shanghai 200030,China);Synthesis,Modiffication and Applications of Poly (ε-caprolactone)[J];POLYMER BULLETIN;2000-02
8 ZHAO Yu1,2, LI Bing-yan3, CHAO Yong-lie1, DU Li1. (1. Dept. of Prosthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, China;2. Dept. of Prosthodontics, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou 510055, China;3. Dept. of Articulation, West China College of Stomatology, Sichuan University, Chengdu 610041, China);Effects of simulating static magnetic fields of magnetic attachments on osteoblasts proliferation, cell cycle distribution and apoptosis ratio[J];West China Journal of Stomatology;2007-05
9 XU Chun,FAN Zhen,CHAO Yong-lie,DU Li,LIU Wei-cai,ZHANG Fu-qiang.Department of Prosthodontics, Affiliated Ninth People's Hospital,Medical College,Shanghai Jiaotong University·Shanghai Research Institute of Stom-atology, Shanghai 200011, China;;The effects of static magnetic fields generated by magnetic attachments on morphologies of human gingival fibroblasts[J];Journal of Clinical Stomatology;2007-02
10 ZHANG Ting1,2 XIONG Zhuo1,2 YAN Yongnian1,2 ZHANG Renji1,2 PAN Jiluan1,2 MA Chao1,2 (1.Department of Mechanical Engineering,Tsinghua University,Beijing 100084;2.Key Laboratory for Advanced Materials Processing Technology of Ministry of Education,Tsinghua University,Beijing 100084);Two Dimensional Mechanical Training of Tissue Engineered Cardiac Patch in Vitro and Simulation[J];Journal of Mechanical Engineering;2010-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 JING Ai-ping ,HUANG Wei-zu ,LI Ying ,et al.Beijing Geriatrics Hospital,Beijing 100095,China;A clinical study: PEMFS as therapeutic method for osteoporosis[J];Modern Rehabilitation;2001-24
2 XIANG Qun,WANG Xian ping,SUN Wenmin,et al.Yancheng First People's Hospital,Yancheng 224001,China;Interventional treatment of pulsed electromagnetic fields(PEMFs) on senile osteoporosis[J];Modern Rehabilitation;2002-03
3 Xia Qun,Feng Yuanming,Jin Hongbin,et al.Tianjin Accident Emergency Center(300211);Fracture Healing and Blood Calcium Phosphorus Metabolism Influenced by Pulse Electromagnetic Fields[J];CHINA JOURNAL OF ORTHOPAEDICS AND TRAUMATOLOGY;1999-04
4 Wang Heming,Yan Mengning,Lai Yulian.Fujian College of Traditional Chinese Medicine (Fuzhou 350003);Effects of Frequency modulation Pulse Electromagnetic Fields on Fracture Union in Rabbits[J];CHINA JOURNAL OF ORTHOPAEDICS AND TRAUMATOLOGY;1999-04
5 GUO Ruihua,HUANG Saiqing,JIA Hongmei, et al Shanghai Sixth People's Hospital,200233;An experimental study on pulsing electromagnetic field therapy in peripheral nerve regeneration[J];CHINESE JOURNAL OF REHABILITATION MEDICINE;1999-03
6 KE Li,CHEN Lulu,TU Yihui,et al.Wuhan Fourth Hospital,Wuhan 430033,China;Effect of pulsing electromagnetic fields on prevention of osteoporosis in ovariectomized rats[J];Chinese Journal of Osteoporosis;2001-03
7 WENG Yingxia, GAO Qiaoying , SHAO Hongwei, et al. The 85 Hospital of PLA, Shanghai 200052, China;Osteoporotic pain and effectiveness of pulsed electrical magnetic fields in treating pain in 126 patients with osteoporosis[J];Chinese Journal of Osteoporosis;2003-04
8 JIANG Youzhao,CHEN Bing.Department of Endocrinology,South-western Hospital,The Third Military Medical University,Chongqing 400038,China;Shortterm effect of pulsed electro-magnetic fields on management of osteoporosis[J];Chinese Journal of Osteoporosis;2005-03
9 CHEN Lulu,KE Li,ZENG Tianshu, et al. Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;Influence of pulsing electromagnetic fields on bone histomorphometry of ovariectomized rats[J];Chinese Journal of Physical Medicine and Retabulitation;2001-04
10 Wen Jun,Wei Ming,Wang Sigang,et al. Department of physics,The fourth military medical university, Xi'an;Effects of ELF Pulsatile Electromagnetic Fields on Viscosity and Coagulation of Blood[J];;1996-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved