Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《West China Journal of Pharmaceutcal Sciences》 2002-01
Add to Favorite Get Latest Update

STUDIES ON EXTRACTION AND SEPARATION OF TOTAL FLAVONOID OF HERBA EPIMEDII

MA Hui ping,JIA Zheng ping,XIE Jing wen,GE Xin,HE Xiao ying,,XIE Hua Department of Pharmacy,Lanzhou General Hospital,PLA,Lanzhou 730050,China  
OBJECTIVE To obtain the best extraction,separation and purification methods of total flavonoid from Herba Epimedii .METHODS The orthogonal design of four factors and three levels were used to elect the optimum extraction method.Three methods as ethyl acetate extraction,acid alkali sedimentation and lead salt sedimentation were compared to determine the most efficient one.RESULTS The factors influenced the extraction efficiency were in following order:solvent,times,time and solvent volume.The optimum extracting condition was to add 10 times amount of 70% ethanol,reflux and extract for 1.5 h,three times totally.It indicated that acid alkail sedimentation was the most efficient and methanol can be used as ideal solvent.CONCLUSION The best extraction process is stable and easy to operate,which is suit to industrialized production.
【Key Words】: Herba Epimedii Total flavonoid of Herba Epimedii Extraction Separation Purification
【CateGory Index】: R284
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 CHAI Yi feng ,WU Yu tian andLI Xiang(Department of Pharmaceutical Analysis ,College of Pharmacy ,Second Military edical University ,Guohe Road 32 5 , Shanghai 2 0 0 4 33);Pharmaceutical Analysis (Ⅱ)[J];Analytical Laboratory;2004-07
2 HOU Ji-rui~1, SHENG Ji-min~2, WANG Xiu-quan~1, TIAN Yi-xin~1, WU Gou-ju~1 (1. College of Chinese Medicinal Material, Jilin Agricultural University, Changchun, Jilin 130118, China; 2. Forestry Bureau of Changbai County, Changbai, Jilin 134400, China );Advances in Studies on Herba epimedii[J];Journal of Jilin Agricultural University;2004-01
3 LI Feng-lin,LI Qin-wang,GAO Da-wei1,FENG Cai-ning2,SHAO Jun-jie2(1 Environment & Chemical Engineering School,Yanshan University,Qinhuangdao,Hebei,610400;2 Animal Institute of Science & Technology,Northwest Agricultural University,Yanglin,Shanxi,712100;3 Bioengineering Department,Jilin Agricultural Institute of Science and Technology,Jilin,Jilin,132101);Research development on the isolation and purification of natural flavonoid[J];Jiangsu Condiment and Subsidiary Food;2008-05
4 SU Wen-qiang~1, YANG Lei~1, LI Yan-jie~2, ZHU Ming-hua~1, CHEN Qi-fan~3(1.College of Material Science and Engineering,Northeast Forestry University,Harbin 150040,China;2.Sixth Pharmaceutical Factory of Harbin Medicine Group,Harbin 150056,China;3.Experiment Center of Liaodong College,Dandong 118003,China);STUDY ON EXTRACTION OF RESVERATROL FROM POLYGONUM CUSPIDATUM SIEB.ET ZUCC BY ALKALI SOLUTION[J];Journal of Chemical Industry of Forest Products(Bimonthly);2004-01
5 XIAO Kun-fu~1,LIAO Xiao-feng~2(1.Department of Chemistry,Lishui College,Lishui 323000,China;2.Department of Biology,Donghua College of Technology,Fuzhou 344000,China);Isolation and Structural Identification of a Flavonoid from Lithocarpus polysachyus Rehd.[J];Chemistry and Industry of Forest Products;2006-03
6 ZHENG Xu-dong HU Hao-bin ZHANG Ke-jun (Dept.of Chem.and Chem.Engineering,Longdong College,Qingyang 745000,China);Determination of Total Flavonoids in"Geng-nian-le"Tablets[J];Physical Testing and Chemical Analysis(Part B:Chemical Analysis);2008-09
7 GAO Jian-ping,WANG Yu-hua,JIA Guo-xian(Department of Pharmacy,Inner Mongolia Medical College,Hohhot 010059 China);ULTRASONIC EXTRACTION TECHNIQUE OF TOTAL FLAVONE FROM CAMELLIA[J];Acta Academiae Medicinae Neimongol;2009-01
8 CHEN Yue-hong1,WANG Jian-ping1,JIANG Hai-qiang2(1.College of Pharmacy,2.Experiment Center,Shandong University of Traditional Chinese Medicine,Jinan 250014,China);Optimization of Extraction Technology of Total Flavonoids from Mulberry Leaves by Orthogonal Design[J];Food and Drug;2008-03
9 ZHONG Wei-yan1,JIANG Hai-qiang1,WANG Jian-ping1,ZHANG Tao2,YU Peng-fei1(1.Shandong University of Traditional Chinese Medicine,Jinan 250355,China;2.Academy of Military Medi-cal Sciences,Beijing 100850,China);Extraction and Isolation of Antioxidative Principle from Radix Puerariae lobatae[J];Food and Drug;2008-09
10 Chen Yan;Optimization of Extracting Conditions of Flavonoids from Sinaraundinaria nitida Leaves[J];Food Science;2003-01
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Wang Changli;Song Xiaomei;et al.(Shanxi COllege of TCM,XIanyang,712083);Study on Extracting Processes for Total Flavone of Herba Epimedii[J];CHINESE TRADITIONAL PATENT MEDICINE;1996-05
2 LiQingnan(LiQN),LiaoJinmin(LiaoJM),WuTie(WuT),etal(BoneBiologyLaboratory,GuangdongMedicalColege,Zhanjiang524023);EpimediumsagitatumMaximpreventinghormone┐inducedosteoporosisinrats[J];CHINESE PHARMACEUTICAL JOURNAL;1996-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 DU Wu-ting et al(Key Laboratory of Environmental Science and Biodiversity Conservation of Sichuan Higher Education,College of Life Science,China West Normal University,Nanchong,Sichuan 637002);Comparison of Total Flavonoid Content in Leaves of Different Age Stems of Epimedium sagittatum[J];Journal of Anhui Agricultural Sciences;2009-06
2 Li Li~1,2 , Dou Jianpeng1, Wu Wei1, Liu Zhiqiang~*1 , Liu Shuying1 ~ 1(Laboratory of New Drug Research, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022) ~ 2(Graduate School of the Chinese Academy of Sciences, Beijing 100039);Study on Flavonoids in Plant Extracts from Epimedium Koreanum Nakai. by Liquid Chromatography-Electrospray Ionization Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2005-03
3 GUO Su hua,HUANG Jian fu,ZHENG Lian mei,et al\;(Dept.of Pharmacy,FCTCM,Fuzhou,Fujian 350003,China) \;;Study on kamtschaticum Fisch and Its Preparations[J];Journal of Fujian College of Traditional Chinese Medicine;2001-04
4 ZENG Yanhui ZHANG Zemei LIN Yongcheng The Second People's Hospital of Shantou City, Guangdong Guangdong Shantou,515011;Research on Bone Metabolizing Biochemistry Quota Check and Epimedium Effect of the Postemenopansal Osteoporosis Patient[J];International Medicine & Health Guidance News;2005-06
5 Wang Rui-Yan, Xie Gui-Zhi (Department of Nephrology, Daqing Oilfields Genaral Hospital, Heilongjiang);Clinical Observation on Treating Ⅲ~Ⅳ Stage IgA Nephropathy by Suqiheji Combine[J];World Clinical Drugs;2007-07
6 BO Liya;WU Chunsheng;WANG Xiuxia The 3rd Hospital of Hebei Medical University, Shijiazhuang 050051, China;Clinical observation on the use of“Gu Shu Kang”in treatment of osteoporotic compression fracture of the spine[J];Chinese Journal of Bone Tumor and Bone Disease;2003-05
7 LI Liang-yao, YE Li-ping, YAN Xiong, DIAN Ling-hui, WU Tie (Guangdong Medical College, Dongguan 523808, China);Study on New Technique of Extracting Total Flavonoid of Epimedium sagittatum Maxim[J];Guangzhou Chemical Industry;2008-04
8 ZHANG Jun-ying,HUANG Luo-sheng,CHEN Jian 1,CHEN Jie(TCM college of CPU, nanjing 210038, China);Study on Alcohol Extraction Process of Herba Epimedii[J];Strait Pharmaceutical Journal;2004-01
9 WANG Shumei,GAO Zhenqiang,YIN Lixin.Department of Pharmacy,The Second Hospital of Hebei Medical University,Shijiazhuang 050000;Effect of gushuling-slow release micro balloons on ovariectomized osteoporotic rats[J];Hebei Journal of Traditional Chinese Medicine;2006-10
10 SHEN Xia,ZHANG Wen-qing~,WO Shao-jing(School of Chemistry and Pharmaceutics, East China University of Science and Technology, Shanghai 200237, China);Studies on Extracting Factors of Bushen Huoxue Jian[J];Journal of East China University of Science and Technology;2005-02
China Proceedings of conference Full-text Database 1 Hits
1 ZHANG Wen-qing, SHEN Xia (Chemical and Pharmaceutical Institute, East China University of Science and Technology, Shanghai, 200237, China);Study on the chitosan clarification procedure on Bushenhuoxue Solution[A];[C];2004
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Ying-ying et al (College of Animal Science and Technology,Northwest A & F University, Yangling,Shaanxi 712100);Studies on Screening Compound Diluent Prescriptions with Traditional Chinese Medicine for Bull Semen[J];Journal of Anhui Agricultural Sciences;2007-10
2 DU Wu-ting et al(Key Laboratory of Environmental Science and Biodiversity Conservation of Sichuan Higher Education,College of Life Science,China West Normal University,Nanchong,Sichuan 637002);Comparison of Total Flavonoid Content in Leaves of Different Age Stems of Epimedium sagittatum[J];Journal of Anhui Agricultural Sciences;2009-06
3 MA Chao,HAO Qing-nan,MA Bing-gang(Agricultural College,Shihezi University,Shihezi,Xinjiang 832003,China);Separation and Determination of Resveratrol and Its Pharmacological Functions[J];Northern Horticulture;2008-04
4 ZHAO Jun1,2 JIN RiGuang1 LUO Lu1 (1.College of Materials Science and Engineering,Beijing University of Chemical Technology,Beijing 100029;2.National Institute of Pharmaceutical Research & Development Co Ltd,Beijing 102206,China);A correlation between the anti-HIV properties of Chinese medicinal herbs and their trace element content[J];Journal of Beijing University of Chemical Technology(Natural Science Edition);2007-05
5 ZHENG Ke-li,ZHENG Xiao-lin,WU Qing-qing (Department of Chemistry & Biology Engineering,Sanming University,Sanming 365004,Fujian,China);Study on ultrasonic wave aided-extracting resveratrol from Polygonum Cuspidatum[J];Journal of Baoji University of Arts and Sciences(Natural Science Edition);2008-02
6 ZHANG Ru-xiang ZHOU SHi-biao DAI SHeng-wu ZHANG Wei-qin (Department of Chemistry and Chemical Engineer- ing, Hunan University of Arts and Science, Changde Hunan, 415000);The Study on the Extracting Flavonoids from Zingiber Officinalerocs with Orthogonal Experiment[J];;2005-03
7 Jiang Xiang-kun1 Liu Min-ru2(1Shenzhen Maternity and Child Health Hospital Affiliated to Southern Medical University,Guangdong Shenzhen 518028;2 Chengdu University of Traditional Chinese Medicine,Si Chuan Chengdu 610075);Effects of Pharmacological Serum Containing Bushenfufang Capsule on Proliferation,Differentiation and Mineralization of Osteoblasts in Vitro[J];Journal of Chengdu University of Traditional Chinese Medicine;2009-01
8 FU Li-bo1,2,XU Hai-yan2,LIU Feng-lian3,YANG Yan-long1,YANG Yan-ping2(1.Basic Medical College,Jinlin University,Changchun 130021,China;2.College of Life Sciences,Changchun Teachers College,Changchun 130032,China;3.Experiment and Education Technology Center,Linyi Teachers College,Linyi 276005,China);Effects of epimedium on blood pressure in normal and stress-induced rats[J];Journal of Northeast Normal University(Natural Science Edition);2008-01
9 Fu Yan-li Xu Zhong-liang Li Yuan-jun (Harbin No.6 Pharmaceutical Factory,Harbin 150056,China);Research on Refining Process of Epimediun Flavonoids by Macroporous Resin[J];Chinese Journal of Medicinal Guide;2008-04
10 Zhou Dayong, Xu Qing, Xue Xingya, Zhang Feifang, Liang Xinmiao (Dalian Institute of Chemistry and Physics, Chinese Academy of Sciences, Dalian 116023);Analysis of Flavonoid Glycosides in Fructus Aurantii by High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2006-S1
【Secondary Citations】
Chinese Journal Full-text Database 9 Hits
1 Li Baiqing, (Department of Microbiology, Bengbu Medical College, Anhui );EFFECTS OF CHINESE DRUGS INVIGORATING KIDNEY ON CELLULAR IMMUNITY I . Effect of Epimedium and Cistanche on E-rosette formation and activity of acid α——naphthyl acetate esterase in human lymphocyte[J];Journal of Bengbu Medical College;1985-03
2 Liao Jinmin, Li Qingnan, Huang Lianfang, Xie Hua, Liu Chenshu (Bone Biology Laboratory, Guangdong Medical College, Zhanjiang 524023);A EXPERIMENTAL STUDY OF EFFECT OF PREDNISON ACETATE ON RAT SKELETON[J];Chinese Journal of Anatomy;1994-04
3 DING Yan, XING Shan-Tian, ZHOU Jin-Huang (Inst. Pharmac. & Toxic. Academy of Millitary Medical Sciences, Bieniug 100850);Immunopharmacologic effects of Epimedium Polysaccharide and Icarrin on mouse thymus[J];Chinese Pharmacological Bulletin;1993-05
4 XU Lan-Zhi;CHEN Wei-Ning (Department of Pharmacology, Weifang Medical College, Weifang 261042);Blocking effect of total flavones of epimedium on adrenergic receptor[J];CHINESE PHARMACOLOGICAL BULLETIN;1994-04
5 DING Yan;CHEN Ping;XING Shan-Tian;RU Xiang-Bin(Institute of Pharmacology and Toxicology,Beijing 100850);Effect of Epimedium polysaccharide against inhibitory action of azidothymidine on mouse hemopoietic and immune functions[J];CHINESE JOURNAL OF PHARMACOLOGY AND TOXICOLOGY;1994-03
6 XIONG Yue-bin; ZHOU Chu-hua(Jiangxi Institute of Medical Sciences),Nanchang 330006;The effect of extracts from herba epimedii and semen cuscutae on thefunction of male reproduction[J];CHINESE PHARMACEUTICAL JOURNAL;1994-02
7 Lia Fuchua, Ding Guangxia and Li Juxian (Changhai Hospital, Second Military Medical University, Shanghai 200433);Effects of Epimedium sagittatum Maxim. Polysaccharides on DNA Synthesis of Bone Marrow Cells of "Yang Deficiency" Animal Model Caused by Hydroxyurea[J];China Journal of Chinese Materia Medica;1991-10
8 Cheng Qing -li;Chen Xiang -mei;Shi Suo-zhu,et al.( Deportment of Nephrology,General Hospital of Chinese PLA ,Beijing 100853);EFFECTS OF EPIMEDIUM SAGITTATUM ON IMMUNOPATHOLOGY ANDEXTRACELLULAR MATRICES IN RATS WITH CHRONIC RENAL INSUFFICIENCY[J];CHINESE JOURNAL OF INTERNAL MEDICINE;1994-02
9 Shan Yuqing, et al(Institute of Medicinal Biotechnology, Beijing);Effect of Flavone Glycosides of Epimedium koreanum on Murine Fibrinolytic System and Apoplectic Mortality[J];Acta Academiae Medicinae Sinicae;1992-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved