DETERMINATION OF RUTIN IN FLOS SOPHORAE AND FRUCTUS SOPHORAE BY HPCE
CHEN Yong-chuan,MU Hai-chuan,LIU Song-qing Southwest Hospital,the Third Millitary Medical College,Chongqing 400038,China
OBJECTIVE To develop an HPCE method for determining rutin in Flos Sophorae and Fructus Sophorae .METHODS The Measurements were carried out in a buffer solution containing 50 mmol·L -1 boric acid solution and 50 mmol·L -1 SDS(pH 8.0),at 20 kV running voltage applied to a 50 cm×50 μm capillary column.The detection wavelength was 260 nm.RESULTS The linear range of rutin is 0.016 to 0.750 mg·ml -1 , RSD was 2.49% to 2.76%.The recovery of rutin was 98.16% to 99.05%. CONCLUSION The method is simple,rapid,economic and accurate.
【CateGory Index】： R284