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Determination of alkaloids and gentiopicroside in Radix Gentianae Macrophyllae by HPLC

WEI Xin~1,HE Hong-Sheng~2,WANG Shu~(1*)(1.West China School of Pharmacy,Sichuan University,Chengdu 610041,China;2.Department of Pharmacdogy,Beijing Changping Hospital,Beijing 102200,China)  
OBJECTIVE To establish a method for the determination of gentianine,gentianal and gentiopicroside in Radix Gentianae Macrophyllae.METHODS HPLC was adopted to determine the contents in Radix Gentianae Macrophyllae.RESULTS This method had good linearity in the range of 3.28-16.92 μg(R~2=0.9991),and the average recovery was 94.42% with RSD of 2.04% for gentianine.It had good linearity in the range of 0.05-4.87 μg(R~2=0.9994),and the average recovery was 95.87% with RSD of 1.66% for gentianal.It was linear in the range of 79~390 μg(R~2=0.9994),and the average recovery was 92.86% with RSD of 2.19% for gentiopicroside.CONCLUSION This method is simple,accurate,and suitable for the quality control of Radix Gentianae Macrophyllae.
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