Determination of cinnamaldehyde and cinnamic acid in Cinnamon formula granule by HPLC
CHENG Cha-qun1,WEI Yan-hua1,TAN Xiu-fen1,ZENG Rui2 ( 1. Affiliated Hospital of Fangcun Third Branch Guangzhou University of Chinese Medicine,Guangzhou,Guangdong,510045 P. R. China; 2. Ethnic Pharmaceutical of Southwest University for Nationalities,Chengdu,Sichuan,610041 P. R. China)
OBJECTIVE To establish a method to determine cinnamaldehyde and cinnamic acid in Cinnamon formula granule. METHODS HPLC method was adopted. ODS column( 250 mm × 4. 6 mm,5 μm) of Hydrosphere with the mobile phase containing a mixture of acetonitrile water -0. 1% phosphoric acid solution ( 35: 25: 40) ,with a flow rate of 1. 0 mL·min -1,and the wavelength at 287 nm,were utilized for this study. RESULTS The method could quantitatively determine cinnamaldehyde and cinnamic acid in Cinnamon formula granule. The linear range of cinnamaldehyde was 0. 75 7. 50 μg ( r = 0. 9995) and the average recovery was 98. 2% with RSD of 1. 61% . The linear range of cinnamic acid was 0. 22 1. 32 μg( r = 0. 9992) and the average recovery was 97. 9% with RSD of 1. 32% . CONCLUSION The method is accurate,sensitive and can be used to control the quality of Cinnamon formula granule.