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《West China Journal of Pharmaceutical Sciences》 2012-04
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Effects of icariin on liver microsomal cytochrome P450 in rats with different ages

LI Li-sheng,CHEN Lan,WANG An-bin,LU Yuan-fu,LIU Jie,SHI Jing-shan(The Key Laboratory of Basic Pharmacology of Guizhou Province,Department of Pharmacology,Zunyi Medical College,Zunyi,Guizhou,563099 P.R China)  
OBJECTIVE To compare the effects of icariin on liver microsomal cytochrome P450 in the rats with different ages.METHODS After intragastric administration of icariin(60 mg·kg~-1) for 4 weeks,Liver microsomes of six-month-old and eighteen-month-old SD male rats were extracted by calcium precipitation method.The cytochrome P450 content was measured through carbon monoxide reductive difference spectrometry assay.The activities of aniline hydroxylase(CYP2E1) and erythromycin-N-demethylase(CYP3A) were detected colorimetrically.ELISA was used to determined the content of CYP1A1,CYPb_5.Expression of CYP1A1,CYP2A3,CYP2E1,CYP3A1,CYP3A2 and CYP4B1 mRNA were detected by real-time RT-PCR.RESULTS Compared with controls,contents of CYP450 and CYP1A1,activities of Erythromycin-N-demethylase and the expression of CYP1A1,CYP3A1 and CYP3A2 mRNA were increased remarkably by icariin.In contrast,the activity of aniline hydroxylase and CYP2E1 mRNA expression were decreased.There was no significant diference of above-mentioned changes between six-month-old and eighteen-month-old rats.The content of CYPb5 and expression of CYP2A3,CYP4B1 mRNA were unchanged.CONCLUSION 60 mg·kg~-1 icariin can induce CYP450,CYP1A1 and CYP3A,inhibit CYP2E1,but age-difference has no effect.
【Key Words】: Icariin Cytochrome P450 CYP1A1 CYP2E1 CYP3A CYPb5
【Fund】: 国家自然科学基金资助项目(批准号:81160400);; 贵州省科学技术基金[黔科合J字(2009)2146号]
【CateGory Index】: R285.5
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【References】
Chinese Journal Full-text Database 1 Hits
1 ZHOU Kun;ZHU Tao-tao;ZHANG Yue;DAI Zhi;Key Laboratory of Traditional Chinese Medical Formulae, Ministry of Education, Institute of Traditional Chinese Medicine,Tianjin University of TCM;R&D Center of Sanjiu Medical & Pharmacutical Co. Ltd;;Influence of Zhuanggu Guanjie pill on liver microsome cytochrome P450 in rats[J];Tianjin Journal of Traditional Chinese Medicine;2014-11
【Citations】
Chinese Journal Full-text Database 1 Hits
1 LI Li,WANG Xue-mei(Department of Integrated Chinese and Western Medicine,First Hospital,Peking University,Beijing 100034,China);Progress of pharmacological research on icariin[J];China Journal of Chinese Materia Medica;2008-23
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Qing-jie et al(Jilin Tianyao Technology Co.,Ltd,Changchun,Jilin 130012);Study on Extraction of Compound Luronggubao Capsule[J];Journal of Anhui Agricultural Sciences;2011-12
2 LIU Ke-pan1,WANG Li-feng2,LI Yang1,YANG Bin1,DU Chao1,WANG Yang1* 1.College of Chinese Materia Medica,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China 2.Department of Experimental Teaching,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Preparation,Pharmacokinetics,and Tissue Distribution Properties of Icariin-Loaded Stealth Solid Lipid Nanoparticles in Mice[J];中草药(英文版);2012-02
3 ZHAO Bizhou;TIAN Jialing;YU Zhanhai;HU Yongshou;MA Haibing;Department of Stomatology in Gansu Provincial Hospital of Traditional Chinese Medicine;School of Stomatology in Lanzhou University;;Effects of Icariin on the Expression of OCN in Human Periodontal Ligament Cells[J];Western Journal of Traditional Chinese Medicine;2014-01
4 ZENG Xiao;YUAN Ding;WANG Ting;DI Guo-jie;LIU Miao-miao;LI Jin;LIU Zhao-qi;ZHANG Chang-cheng;Medical College of China Three Gorges University;;The recovery effects of total flavones from Epimedium on the spermatogenesis in cryptorchid mice with orchidopexy[J];Guangdong Medical Journal;2014-02
5 WANG Dong,ZHANG Jiang,WU Tongru,et al Shuguang Hospital,Shanghai University of TCM,Shanghai(200021);The Effect of Utility Monomer in Epimedium to the Renal Fibroblast Cell Line and Mesangial Cell Line[J];Chinese Journal of Integrated Traditional and Western Nephrology;2012-11
6 SONG Yi-xiang;LI Wei-wei;WANG Xue-mei;East-west Integrative Medicine Research Center,Peking University First Hospital;;Adances in neuroprotective effects mechanisms of Icariin[J];Global Traditional Chinese Medicine;2014-04
7 SONG Xiaolin;GU Ying;Lianyungang Houspital of Traditional Chinese Medicine;Guanyun Houspital of Traditional Chinese Medicine;;Study on Qualitys Standards of Yixhen Huichun Pills[J];Journal of Liaoning University of Traditional Chinese Medicine;2014-09
8 LIU Tian-lin;ZHANG Xue-bin;ZHANG Xin;PAN Ting;WU Yan-ling;HUANG Yuan-liang;Laboratory of Oral Biomedical Science and Translational Medicine,School of Stomatology,Tongji University;Department of Stomatology,Oriental Hospital,Tongji University;Department of Dental Implant,Yantai Hospital of Stomatology;;Effect of Icariin on the Proliferation,Osteogenic Differentiation,and Adipogenic Differentiation of Canine Bone Marrow Mesenchymal Stem Cells[J];Journal of Oral and Maxillofacial Surgery;2014-04
9 DU Shao-bin;Pharmacy Department,First Attached Hospital of Bengbu Medical College;;Research Advances in Anti-Erectile Dysfunction of Epimedium[J];Chinese Journal of Ethnomedicine and Ethnopharmacy;2014-22
10 BAO Yu,SUN Run-Guang(School of Physics and Information Technology,Shaanxi Normal University,Xi′an 710062,China);Screening of Effective Ingredients of Traditional Chinese Herb Epimediumkoreanum Nakai by Combination of Co-cultured ECV304/C6 Cells as an in vitro BBB Model with HPLC-MS Analysis[J];Chinese Journal of Biochemistry and Molecular Biology;2012-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yuzhe(Pre-clinical College,Dali University,Dali,Yunnan 671000,China);In Vitro Study of Rat Coliform Bacteria Metabolism of Steroidal Sponin Extract from Anemarrhena asphodeloides[J];Journal of Dali University;2012-03
2 DOU Zhi-Hua1,LUO Lin2,DING An-Wei3,WANG Lu-Jun1,ZHANG Bing1,Li Min1 1Department of Pharmacy,Nantong Third Affiliated Hospital,Nantong University,Nantong 226006,China,2Department of Pharmacology,Medical School,Nantong University,Nantong 226001,China,3Institute of Materia Medica,Pharmacy School,Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China;Profile-effect between fingerprint and liver protection of serum containing compound Wurenchun capsules[J];Journal of the Fourth Military Medical University;2008-02
3 QIU Xiang-jun1,WANG Zhe2, XU Tao2,LUO Shun-bin2, HU Guo-xin2 (1.Medical College of Henan University of Science and Technology,Luoyang 471003,Henan Province,China;2.School of Pharmacy of Wenzhou Medical College,Wenzhou 325027,Zhejiang Province,China);Determination of the activity of CYP3A in rat liver microsomes by HPLC[J];The Chinese Journal of Clinical Pharmacology;2012-11
4 LIU Ji-xin;GAO Qing-jian;LU Cheng;WANG Rui;TANG Hao;Department of Pharmacy,Gansu Provincial Hospital;Department of Pharmacology,Ningxia Medical University Pharmacy College;;Impacts of isomers-psoralen and isopsoralen on HepG2 cells and cytochrome P450 enzymes[J];The Chinese Journal of Clinical Pharmacology;2014-05
5 SHEN Chanjuan1,XIE Chang1,LIAO Pengcheng1,YE Xiaochuan1, CHEN Shuhe1,2,ZAN Junfeng1,2,LIU Yanwen1 1Hubei University of Traditional Chinese Medicine,Wuhan 430061,China 2Medicine Hospital of Hubei Province,Wuhan 430061,China;Pilot Study on Serum Pharmacochemistry of Poria[J];Medical Journal of Wuhan University;2012-04
6 ZHANG Ning1,YU Wen-juan1,WANG Xiang-ling1,LIN Mao2,QIU Jun-qiang1,YANG Xian-le1,3(1.Aquatic Pathogen Collection Center of Ministry of Agriculture,Shanghai Fisheries University,Shanghai 200090,China;2.Fisheries College,Jimei University,Xiamen 361021,China;3.E-Institute of Shanghai Municipal Education Commission,Shanghai 200090,China);Cytochrome P450 contents and activity in hepatic microsome of grass carp(Ctenopharyngodon idellus)[J];Marine Fisheries;2007-02
7 ;Research development of cytochromo P450[J];Journal of Jilin Military Medical College Fourth Military Medical University;2005-03
8 QIU Furong~1,ZHANG Rong~2,SUN Jianguo~2,WANG Guangji~2,JIANG Jian~1,MA Yueming~3(1 Department of Clinical Pharmacology,Shuguang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China; 2 Key Laboratory of Pharmacokinetics,China Pharmaceutical University,Nanjing 210038,China;3 Laboratory of Pharmacokinetics, Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China);Determination of 6β-hydroxytestosterone and 1-hydroxymidazolam in human liver microsomes by HPLC[J];Chinese Journal of Clinical Pharmacy;2010-03
9 YANG Xi~(1,2),GU Zhengyi~1,SHA Xianyi~3,HE Jinhua~1,FANG Xiaoling~3(1 Department of Pharmaceutics,Xinjiang Medical University,Urumqi 830054,China;2 Medicinal Institute of Xinjiang Uygur Autonomous Region,Urumqi 830004, China;3 Department of Pharmaceutics,School of Pharmacy,Fudan University,Shanghai 201203,China);Absorption mechanism of hyperoside across Caco-2 cells model[J];Chinese Journal of Clinical Pharmacy;2012-02
10 ZHANG Haipeng 1) ,ZHAO Jie 1) ,ZHANG Lirong 2) ,WANG Xiaofei 2) ,XUE Wenhua 1) 1) Department of Pharmacy,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2) Department of Pharmacology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001;Establishment of a HPLC method to detect CYP3A4 activity in human hepatic tissue[J];Journal of Zhengzhou University(Medical Sciences);2013-04
【Secondary Citations】
Chinese Journal Full-text Database 9 Hits
1 Li Shutong; Li Tiejun; Zheng Qinyue; Wang Hongbin (Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai,200433);Effects of icariine on lymphocyte proliferation and production of colony stimulating factor-like activity from mice splenocytes in vitro[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1995-04
2 Li Shutong;Li Tiejun;Zheng Qinyue;Wang Hongbin(Department of Pharmacology,College of Pharmacy,Second Military Medical University,Shanghai,200433);Effects of icarrin on function of murine peritoneal macrophages[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1995-06
3 Qin Luping, Shi Hanping, Zheng Shuiqing, Li Bohua, Hong Qiubing, Wang Hongbin, Zhang Jiaqing (Department of Pharmacognosy, College of Pharmacy,Second Military Medicial University, Shanghai, 200433);Effects of osthol and icariin on serum thyroid hormone in mice of hypothyroidism type kidneyyang insufficiency[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1998-01
4 LI Li~ 1 , ZHOU Qi-xin~ 1 , SHI Jing-shan~ 2 (~ 1 Research Center of Biochemistry and Molecular Pharmacology, Chongqing University of Medical Sciences, Chongqing 400016, ~ 2 Department of Pharmacology, Zunyi Medical College, Zunyi 563003, China);Effect of icariin on expression of cytochrome C oxidase subunit Ⅱ mRNA in mice of cerebral ischemia/reperfusion[J];Acta Academiae Medicinae Militaris Tertiae;2006-07
5 Ge Lin fu,DONG Zheng jun,JIANG Guo sheng,et al.General Hospital of Jinan Command PLA,Jinan 250031,China;Experiment Study on the Effect of ICA on HL-60 Cells in Vivo and in Vitro[J];Journal of Qilu Oncology;2001-06
6 ;.NULL.[J];Sichuan Journal of Physiological Sciences;2004-04
7 Wang Min Liu Chongming Zhang Baofeng Department of Physiology;EFFECTS OF EPIMEDIUM ICARIINE ON RABBIT AND DOG CEREBRAL BLOOD FLOW[J];;1991-04
8 CHEN Hong, ZHANG Xiu-zhen(1.Dept. of Medicine,Tongji University Hospital,Shanghai 200092;2.Dept. of Endochrinology,Tongji Hospital,Tongji University,Shanghai 200065,Chin a);Icariin Regulates Expression of Cytokines in Osteoblasts[J];Journal of Tongji University (Medical Science);2005-02
9 LI Shu-mei,SONG Li-ge, ZHANG Xiu-zhen (Dept. of Endocrinology, Tongji Hospital, Tongji University, Shanghai 200065,China);Effects of Icarrin on Osterix in Rat Osteoblast[J];Journal of Tongji University (Medical Science);2005-06
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