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《West China Medical Journal》 2006-03
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Model Construction of Ischemic Postconditioning on Renal Warm Ischemia-reperfusion Injury in Rat

TANG Tie-long~1,LU Yi-ping~(1*),ZHOU Ling~2,et al.(1.Department of Urology,West China Hospital,Sichuan University,Chengdu 610041;2.Department of Anesthesiology,West China Hospital,Sichuan University,Chengdu 610041)  
Objective:To construct the model of ischemic postconditioning(Pos-con)on renal ischemia-reperfusion injury(IRI)in rat.Methods:After right nephrectomy,the renal pedicles of rats were clamped 0min(control),30min,45min and 60min with micro-artery clamps,respectively.After 24h of reperfusion,the blood urea nitrogen(BUN)and serum creatine(Scr)were measured to decide the IRI model.40 Wistar rats were randomized into 4 groups:group A(Control),group B(I/R),group C(Pos-con1) and group D(Pos-con2).After 24h reperfusion without control,the blood samples were taken through the vena cava and the renal samples were taken too.Serum urea nitrogen and creatine were measured.The renal samples were examined by HE stain under light microscopy.Results:Compared with the control group,60min ischemia and 24h reperfusion can increased the BUN and Scr significantly(P0.05).Compared with IRI,the BUN and Scr of Pos-Con1 were decreased remarkably(P0.05),the injury of kidney was attenuated and the pathological scale was reduced significantly(P0.05).However,Pos-con2(didn't) take the same progression.Conclusion:Stable model of ischemic postconditioning on renal IRI in rat can be prepared by Pos-con1.
【CateGory Index】: R692
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