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《Journal of Harbin Medical University》 2006-05
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The investigation on expression,purification and restore of endostatin in Escherichia coli

HE Jing-jing,JIN De-jun,LIU Xing-han,et al(Department of Otolaryngology,The Second Clinical College,Harbin Medical University,Harbin 150081,China)  
Objective To explore the optimal method for the expression,purification and refolding of rhES in Escherichia coli.Methods The engineered bacteria carrying rhES genes was activated and induced.Exclusion bodies were extracted and washed,and then rhES was degenerated,refolded and concentrated.The activity of rhES was examined by the experiment of CAM.Results The purity of rhES surpassed 95%.In the experiment of CAM,it was shown that rhES could obviously keep new bloodvessels from growing.Conclusion The experiment method provided by this paper can help extract highly-activated,highly-purified and soluble rhES,which will lay a foundation for large-scale production of rhES.
【Fund】: 黑龙江省自然科学基金重点项目(D0340)
【CateGory Index】: R91;TQ464
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 FENG Yi\+1\ CUI Li\|Bin\+2\ LIU Chuan\|Xuan\+1\ MA Qing\|Jun\+1 1(Beijing Institute of Biotechnology, Beijing, 100850, China) 2(University of Maryland, Nutrition and food science department, College Park, Maryland 20742);Inhibition Effect in vitro of Purified Endostatin Expressed in Pichia pastoris[J];Chinese Journal of Biotechnology;2001-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Qun *1, Shi Jing 2, Zhang Huan 2, Ni Jiazuan 3 1(Pharmaceutical College, Shanghai Jiaotong University, Shanghai 200030) 2(Changchun Life Gene Pharmaceutical Limited Company, Changchun 130022) 3(Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022);Study on the Interaction between Recombinant Endostatin with Aloe by Fluorescence and Circular Dichroism Methods[J];Chinese Journal of Analytical Chemistry;2005-07
2 WANG Qun 1, NI Jia-Zuan 1,2* (1. State Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; 2. College of Life Science, Shenzhen University, Shenzhen 518060, China);Effect of Lanthanides on the Interaction Between Recombinant Endostatin and Heparin[J];Chemical Research In Chinese Universities;2004-10
3 FNEG Yi\ MA Qing\|Jun (Beijing Institute of Biotechnology, Key Lab. of Molecule Genetics, Beijing 100850, China);Advance in the Researh of Endostatin[J];Chinese Journal of Biotechnology;2001-05
4 SU Zhi-jian+1 WU Xiao-ping+1 ZHENG Qing+1 FENG Ya+2 YU Ping-ye+2 QU Hong-yan+2 LIU Tai-sheng+2 LI Xiao-kun+1 (1 School of Pharmacy of Jinan University,Guangzhou 510630,China; 2 Biopharmaceutical Research and Development Center of Jinan University,Guangzhou 510632,China);High Expression of Human Endostatin in Pichia pastoris[J];Progress In Biotechnology;2004-05
5 FENG Yi ZHANG Yan-hong FENG Yu-mei MA Qing-jun(Beijing Institute of Biotechnology Beijing 100850,China);Research about Soluble Expression of Human Endostatin in E.coli[J];Progress In Biotechnology;2004-09
6 FENG Yi 1 ,FENG Yu-mei 2 ,ZHANG Yan-hong 1 ,LIU Chuan-xuan 1 ,MA Qing-jun 1 (1.Institute of biotechnology,Beijing100850;2.Chongqing Medical University,China);The preliminary determination of anti-endostatin monoclonal antibody hybridoma cell line[J];Letters in Biotechnology;2002-04
7 FENG Yu-mei, FENG Yi, ZHANG Yan-hong , REN Lie , ZENG Zhao-chun , MA Qing-jun Beijing Institute of Biotechnology, Department of Genetic Engineering , Beijing 100850; Department of Biochemistry and Molecular Biology, Chongqing University of Medical Science, Chongqing 400016, China;Preparation and identification of the monoclonal antibody against human en- dostatin[J];Journal of Cellular and Molecular Immunology;2003-06
8 Feng Yi,Feng Yumei,Zhu Xudong,Ma Qingjun.Beijing Institute of Biotechnology,Genetic Engineering Laboratory,Beijing 100850,China;Inhibition of rhEndostatin on corneal neovascularization[J];Chinese Ophthalmic Research;2005-01
9 YUAN Zhongfang,WEI Zengtao, MU Cuoying, et al. Department of Ophthalmology, Jinan Central Hospital, Jinan 250013, China.;An Experimental Study of the Inhibitive Effect of Recombinant Endostatin on Corneal Neovascularization[J];Chinese Journal of Ophthalmology and Otolaryngology;2004-01
10 Wang Xiaoyan Sun Wensheng Ma Chunhong et al Institute of Immunology,Medica l College of Shandong University,Jinan;Purification of Recombinant Human Secreted Endostatin and Study of Its In hibition Effect on Tumor[J];Chinese Journal of Clinical Oncology;2003-02
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