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《TRANSACTION OF OCEANOLOGY AND LIMNOLOGY》 1998-01
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STUDIES ON PATHOGENICITY OF FOUR VIBRIO STRAINS FOR PENAEUS CHINESIS

Li Tiandao;Yu Jia; Yu Kaikang(Ocean University of Qingdao, Qingdao, 266003) (Yellow Sea Fisherics Research Institute, Qingdao, 266071)  
From August 1995 to April 1996, to compare the effect of Vibrio alginolyticus 91-1, Vibrio parahaemolvticus-91-2, Vibrio anguillarum E3-11, Vibrio furnissii 91-6and their extracellular. products (ECP) on Penales chinensis, normal shrimps were challenged by injecting intramuscularly, injuring and stocking in water with bacteria, feeding with various food, and the shrimps were used for determination of 50% lethal doses (LD50). We observed the histological changes caused by Vibio spp. and their ECPs. TI.e four strains (91--1, 91--2, E3--11, 91-6) had LD50s of1 .2 x 10s, 6.5 x 10z, 4.0 x 10s, 6.8x 109 (CFU/ml) in 24hr, whereas,their LD50s were 6.3 x 105, 8.0 x 106, 2.0 x 107,; 1 .0 x 109 (CFU/ml) in 48hr. .The ECPs of the four strains (91--1, 91--2, E3--11, 91--6) were pathogenic to shrimps, the LD50s were 0. 11, 0.16, 0.23, 0.29 (mg/ml) in 24hr, and 0.05, 0.11, 0.12,. 0.24.(mg./ml)in 48hr. Histopathological observations revealedithat the histological changes were most pronounced in hepatopancreas, gill and stomach etc. Hepatopancreas was most likely to be damaged
【CateGory Index】: S945.4
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