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《Transactions of Oceanology and Limnology》 2012-02
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ACUTE TOXICITY OF CU~(2+) AND EFFECTS OF ANTIOXIDANT ENZYME ON URECHIS UNICINCTUS BODY WALL

LI Jinlong,QIN Zhenkui,SHI Xiaoli,and ZHANG Zhifeng(Key Laboratory of Marine Genetics and Breeding,Ministry of Education, Ocean University of China,Qingdao 266003,China)  
The acute toxicity of Cu2+ to Urechis unicinctus was studied.Cu2+ safe concentration of copper to Urechis unicinctus is 0.0675mg/L,which is higher than the fishery water quality standards,based on the result from median lethal concentrations of copper obtained at 24 h,48 h,72 h,and 96 h.Meanwhile,the activities of antioxidant enzyme in body wall of the worm were detected.The activities of SOD and GPx increased significant(p0.05) in both concentrations(0.25 mg/L,and 0.5 mg/L) at 24 h after the exposure;after that,all the activities of the three antioxidant enzymes decreased in a time-and dose-manner,and the activities of SOD and GPx were lower than that of the control group,the activity of CAT under 0.50 mg/L was significantly lower than the control worm at 96 h after the exposure.This result suggested the activities of GPx and CAT were inhibited by Cu2+ which led the worm to death.
【Fund】: 国家自然科学基金(31072191)资助
【CateGory Index】: X174
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